Human Naïve Pluripotent Stem Cells

ISBN-13: 9781071619070 / Angielski / Twarda / 2021 / 330 str.

Peter Rugg-Gunn

Spis treści

Preface…
Table of Contents…
Contributing Authors...

1. Deriving Human Naïve Embryonic Stem Cell Lines from Donated Supernumerary Embryos using Physical Distancing and Signal Inhibition
Stanley E. Strawbridge, James Clarke, Ge Guo, and Jennifer Nichols

2. Induction of Human Naïve Pluripotency Using 5i/L/A Medium
Laura A. Fischer, Shafqat A. Khan, and Thorold W. Theunissen

3. Induction of Human Naïve Pluripotency Using Chemical Resetting
Peter J. Rugg-Gunn

4. Induction of Human Naïve Pluripotent Stem Cells from Somatic Cells
Constance Onfray, Jia Ping Tan, Stéphanie Kilens, Xiaodong Liu, Jose Polo, and Laurent David

5. Using Microfluidics to Generate Human Naïve and Primed Pluripotent Stem Cells
Irene Zorzan, Onelia Gagliano, Nicola Elvassore, and Graziano Martello

6. Maintenance of Human Naïve Pluripotent Stem Cells
Maria Rostovskaya

7. Generating Trophoblast Stem Cells from Human Naïve Pluripotent Stem Cells
Chen Dong and Thorold W. Theunissen

8. Differentiation and Expansion of Human Extra-Embryonic Endoderm Cell Lines from Naïve Pluripotent Stem Cells
Madeleine Linneberg-Agerholm and Joshua Mark Brickman

9. Capacitation of Human Naïve Pluripotent Stem Cells
Maria Rostovskaya

10. Generation of Pericytic-Vascular Progenitors from Tankyrase/PARP-Inhibitor-Regulated Naïve (TIRN) Human Pluripotent Stem Cells
Ludovic Zimmerlin, Tea Soon Park, Imran Bhutto, Gerard Lutty, and Elias T. Zambidis

11. Profiling DNA Methylation in Human Naïve Pluripotent Stem Cells
Ferdinand von Meyenn

12. Chromatin Profiling of Human Naïve Pluripotent Stem Cells
Adam Bendall and Claudia I. Semprich

13. Mapping Chromatin Accessibility in Human Naïve Pluripotent Stem Cells using ATAC-seq
Jessica K. Cinkornpumin, Ishtiaque Hossain, and William A. Pastor

14. Transcriptomic Analysis of Human Naïve and Primed Pluripotent Stem Cells
Arindam Ghosh and Anup Som

15. Study of X Chromosome Activity Status in Human Naïve Pluripotent Stem Cells using RNA-FISH 
Kasturi Mahadik and Claire Rougeulle

16. Flow Cytometry Analysis of Cell-Surface Markers to Identify Human Naïve Pluripotent Stem Cells
Peter J. Rugg-Gunn

17. Characterizing the Genetic Stability of Human Naïve and Primed Pluripotent Stem Cells
Duncan Baker and Ivana Barbaric

Subject Index List…

Blurb

This volume provides readers with a comprehensive collection of methods to guide them on how to generate, characterize, and use naïve human pluripotent stem cells (hPSCs). The chapters in this book cover topics such as three predominant routes to generate naïve hPSC lines; methods to differentiate naïve hPSCs into specialized cell types; and techniques to characterize naïve hPSCs using key molecular landmarks that benchmark and quality control the cell lines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Cutting-edge and thorough, Human Naïve Pluripotent Stem Cells: Methods and Protocols is a valuable resource for novice and expert researchers who are looking to learn more or expand their research in this developing field.