Human Embryonic Stem Cells: The Practical Handbook

ISBN-13: 9780470033562 / Angielski / Twarda / 2007 / 424 str.

Stephen Sullivan; Chad A. Cowan; Kevin Eggan

Opis

With this valuable practical guide, three members of the Harvard Stem Cell Institute have compiled and edited the definite handbook for the exciting new field of human embryonic stem cell research. The editors have gathered protocols from scientists with extensive reputation and expertise, describing and comparing currently used techniques for the culture of human stem cells and discussing the strengths and weaknesses of the different approaches. Human Embryonic Stem Cells: The Practical Handbook contains the first centralised collection of methods used in human embryonic stem cell biology. The book covers the derivation of human stem cell lines, the obtaining of cells from human stem cell banks, the culturing and characterisation of the cells, and the differentiation of the cells in vitro and in vivo. Lastly, almost all of these protocols can also be used for analyzing and manipulating induced pluripotency iPS stem cells. This allows an even greater number of opportunities for those interested in purusing work in pluripotent stem cells, disease modelling, and other aspects of basic regenerative medicine research. The novel and useful focus of this book sets it apart from other available books:

• Compares and evaluates the protocols used in leading laboratories working on human embryonic stem cells
• Centred solely on practical protocols for human (not mouse) embryonic stem cell research
• Includes extensive troubleshooting sections
• Addresses the different proclivities and behaviours of individual human embryonic cell lines
• Contains techniques currently known only to a small number of specialised laboratories worldwide

This handbook represents an essential source of up-to-date practical information for all cell and developmental biologists working with human embryonic stem cells or wishing to enter the field. It is also essential reading for clinical researchers in areas such as diabetes, cardiovascular disease, and neurological diseases. Praise from the reviews: ..".a highly readable and useful book... A notable feature of the book is its air of openness and honesty... This book... will help many to navigate the uncharted waters of human embryonic stem cell biology." BRITISH SOCIETY FOR CELL BIOLOGY ..". the imaginative solutions in this book can inspire us to get past our most frustrating limitations." CELL STEM CELL ..". the richness in the details of each protocol presented will certainly encourage more scientists to begin studies of Human pluripotent stem cells..." REGENERATIVE MEDICINE "In this fast-moving field, this handbook] will help drive advances of more and more researchers." DIFFERENTIATION ..".a valuable resource for seasoned and novice researchers... an excellent addition to the reference collection of any medical library or research laboratory." THE AMERICAN MEDICAL ASSOCIATION

Recenzje

"A highly readable and useful book .[It] will help many to navigate the uncharted waters of human embryonic stem cell biology."  ( The British Society for Cell Biology Newsletter, Autumn, 2008)

"[It] is a valuable resource for seasoned and novice researchers; it would make an excellent addition to the reference collection of any medical library or research laboratory." (American Medical Association, September 2008)

"...the imaginative solutions in this book can inspire us to get past our most frustrating limitations." (Cell Stem Cell, September 2007)

"...the richness in the details of each protocol presented will certainly encourage more scientists to begin studies of Human ES cells..."  (Regenerative Medicine, 2007)

"In this fast–moving field, this [handbook] will help drive advances of more and more researchers."  (Differentiation, 2007)

"A highly readable and useful book ... [This book] will help many to navigate the uncharted waters of human embryonic stem cell biology." (British Society for Cell Biology, Autumn, 2008)

Spis treści

Foreword. Davor Solter

Preface.

Section 1: Obtaining and culturing human embryonic stem cells.

1 Organization and good aseptic technique in the human embryonic stem cell laboratory.
Minal J Patel, Emma L Stephenson, and Stephen L Minger

2 Sourcing human embryonic stem cell lines.
Glyn Stacey

3 Human embryo culture for human embryonic stem cell derivation.
R. Douglas Powers and Jeannine Witmyer

4 Derivation of human embryonic stem cell lines.
Stephen Sullivan, Dieter Egli, Hidenori Akutsu, Douglas A. Melton, Kevin Eggan and Chad A. Cowan

5 Standard culture of human embryonic stem cells.
Jeremy M. Crook, Rachel Horne, and Alan Colman

6 Culture of human embryonic stem cells in chemically defined conditions.
Julie Hsu Clark and Sheng Ding

Section 2: Characterization of undifferentiated embryonic stem cells.

7 Phenotypic analysis of human embryonic stem cells.
Jonathan S. Draper,  Cheryle A. Séguin and Peter W. Andrews

8 Genetic and epigenetic analysis of human embryonic stem cells.
Laurie A. Boyer, Rudolf Jaenisch, and Maisam Mitalipova

Section 3: Manipulation of human embryonic stem cells.

9 In vivo differentiation of human embryonic stem cells.
Scott A. Noggle, Francesca M. Spagnoli, Ali H. Brivanlou

10 In vitro differentiation of human embryonic stem cells.
A. Henry Sathananthan and Alan Trounson

11 Differentiation of human embryonic stem cells into extraembryonic cell types.
Cheryle A. Séguin and Jonathan S. Draper

12 Directed differentiation of human embryonic stem cells into:
(a) Early endoderm cells.
Kenji Osafune, Alice E. Chen and Douglas A. Melton
(b) Hepatic cells.
Neta Lavon and Nissim Benvenisty
(c) Pancreatic cells.
Hiram Chipperfield

13 Directed differentiation of human embryonic stem cells into:
(a) Cardiomyocytes.
Christine Mummery, Robert Passier and Chris Denning
(b) Endothelial cells.
Carrie Soukup, Shulamit Levenberg and Ondine Cleaver
(c) Osteogenic cells.
Jeffrey M. Karp, Alborz Mahdavi, Lino S. Ferreira, Ali Khademhosseini and Robert Langer
(d) Hematopoietic in vivo repopulating cells.
Shannon McKinney–Freeman, Thorsten M. Schlaeger and George Q. Daley
(e) Lymphocytes.
Petter S. Woll and Dan S. Kaufman
(f) Myeloid cells.
Chantal Cerdan and Mickie Bhatia

14 Directed differentiation of human embryonic stem cells into:
(a) Forebrain neurons.
Emily A. Davis and Lawrence S.B. Goldstein
(b) Dopaminergic neuron.
Jan Pruszak and Ole Isacson
(c) Spinal motor neurons.
Bao–Yang Hu and Su–Chun Zhang

15  (a) Gene targeting: knock out and knock in by homologous recombination.
 Thomas P. Zwaka
(b) RNA interference in human embryonic stem cells.
M. William Lensch, Asmin Tulpule and Holm Zaehres
(c) Generation of gene reporters using bacterial artificial chromosome recombineering. Andrew J. Washkowitz and David A. Shaywitz

Afterword. Azim Surani

Index.

O autorze

Stephen Sullivan: Trinity College, Dublin, Ireland 

Kevin Eggan: Harvard University, Cambridge, MA, USA

Chad A. Cowan: Harvard University, Cambridge, MA, USA

Blurb

Since their isolation in 1998, human embryonic stem (ES) cells have been used as a powerful experimental model for studying the mechanisms of stem cell self–renewal, pluripotency, and differentiation in humans. However, the protocols by which these cells are grown still differ considerably from laboratory to laboratory and standardisation is needed in this young area of research.

Human Embryonic Stem Cells: The Practical Handbook contains all basic methods for sourcing, deriving, culturing, and manipulating human ES cells. The editors have gathered the leading scientists in the field to describe the protocols that they have carried out and tested in their own labs.

The book

• Contains practical information for newcomers to the field: from how to source human ES cells from international cell banks to organizing the laboratory to accommodate work with these cells.

• Provides protocols for routine culture as well as specialized protocols for directing differentiation into specific cell lineages.

• Addresses the different proclivities and behaviours of individual human ES cell lines: authors state which lines they tried their protocols on and, more importantly, which lines worked best.

• Includes troubleshooting sections that address common problems and offer advice on how these can be overcome.

• Follows a simple, concise, and consistent structure: background material and hypothesizing are kept to a minimum to insure clarity and usability at the bench.

Human Embryonic Stem Cells: The Practical Handbook is the essential source of up–to–date, practical information for all researchers and clinicians working with human embryonic stem cells.