Human Cytomegaloviruses: Methods and Protocols

ISBN-13: 9781493962730 / Angielski / Miękka / 2016 / 368 str.

Andrew D. Yurochko; William E. Miller

Opis

With the significant medical role that human cytomegalovirus plays in human disease (from acute disease following primary infection, to chronic disease due to life-long viral persistence), the need to better understand human cytomegalovirus biology and pathogenesis is needed. Human Cytomegaloviruses: Methods and Protocols is designed to be an inclusive document covering all of the techniques and approaches necessary to understand and study the pathobiology of human cytomegalovirus. Topics covered include the history of human cytomegaloviruses, techniques to culture and grow the virus in model cell types, the use of primary cells for the study of human cytomegalovirus pathogenesis, modern molecular techniques for assessing the biological consequences of viral infection, animal models for study of cytomegalovirus replication and numerous other topics of current interest. Written in the successful Methods in Molecular Biology series format, each chapter includes an introduction, list of necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and easily accessible, Human Cytomegaloviruses: Methods and Protocols serves as a tool for basic scientists as well as clinical scientists with an interest in the basic fundamental aspects of viral gene expression and specific aspects of viral pathogenesis.

Spis treści

1. History of the Molecular Biology of Cytomegalovirus

Mark F. Stinski

 

2. Overview of Human Cytomegalovirus Pathogenesis

Maciej T. Nogalski, Donna Collins-McMillen, and Andrew D. Yurochko

 

3. Distinct Properties of Human Cytomegalovirus Strains and the Appropriate Choice of Strains for Particular Studies

Giada Frascaroli and Christian Sinzger

 

4. Use of Diploid Human Fibroblasts as a Model System to Culture/Grow, Study and Understand Human Cytomegalovirus Infection

Elizabeth A. Fortunato

 

5. Use of Recombinant Approaches to Construct Human Cytomegalovirus Mutants

Iryna Dekhtiarenko, Luka Čičin-Šain and Martin Messerle

 

6. The Use of Primary Human Cells (Fibroblasts, Monocytes and Others) to Assess Human Cytomegalovirus Function

Emma Poole, Matthew Reeves, and John H. Sinclair

 

7. Human Hematopoietic Long-Term Culture (hLTC) for Human Cytomegalovirus Latency and Reactivation

Mahadevaiah Umashankar and Felicia Goodrum

 

8. Analysis of Cytomegalovirus Binding/Entry-Mediated Events

Gary C.T. Chan and Andrew D. Yurochko

 

9. Use of 5-Ethynyl-2’-Deoxyuridine Labelling and Flow Cytometry to Study Cell Cycle-Dependent Regulation of Human Cytomegalovirus Gene Expression

Lüder Wiebusch and Christian Hagemeier

 

10. Methods for Studying the Function of Cytomegalovirus GPCRs

Christine O’Connor and William E. Miller

 

11. Methods for the Detection of Cytomegalovirus in Glioblastoma Cells and Tissues

Charles S. Cobbs, Lisa Matlaf, and Lualhati E. Harkins

 

12. Methods to Study the Nucleocytoplasmic Transport of Macromolecules with Respect to Their Impact on the Regulation of Human Cytomegalovirus Gene Expression

Marco Thomas, Barbara Zielke, Nina Reuter, and Thomas Stamminger

 

13. Fluorescence-Based Laser Capture Microscopy Technology Facilitates Identification of Critical In Vivo Cytomegalovirus Transcriptional Programs

Craig N. Kreklywich, Patricia P. Smith, Carmen Baca Jones, Anda Cornea, Susan L. Orloff, and Daniel N. Streblow

 

14. Techniques for Characterizing Cytomegalovirus-Encoded miRNAs

Lauren M. Hook, Igor Landais, Meaghan H. Hancock, and Jay A. Nelson

 

15. What We Have Learned From Animal Models of HCMV

Pranay Dogra and Tim Sparer

 

16. Rodent Models of Congenital Cytomegalovirus Infection

Djurdjica Cekinovic, Vanda Juranic Lisnic, and Stipan Jonjic

 

17. Recent Approaches and Strategies in the Generation of Anti-Human Cytomegalovirus Vaccines

Suresh B. Boppana and William J. Britt

 

18. Approaches for the Generation of New Anti-Cytomegalovirus Agents:  Identification of Protein-Protein Interaction Inhibitors and Compounds Against the HCMV IE2 Protein

            Beatrice Mercorelli, Giorgio Gribaudo, Giorgio Palù, and Arianna Loregian