Human Cell Culture Protocols

ISBN-13: 9781493962082 / Angielski / Miękka / 2016 / 435 str.

Ragai R. Mitry; Robin D. Hughes

Opis

This third edition of Human Cell Culture Protocols expands upon the previous editions with current, detailed protocols for the isolation and culture of a range of primary cells from human tissues. It features cutting-edge methods ready for the laboratory.

Spis treści

1. Introduction to Cell Culture

            Christina Philippeos, Robin D. Hughes, Anil Dhawan, and Ragai R. Mitry

 

2. Isolation and Cultivation of Dermal Stem Cells that Differentiate into Functional Epidermal Melanocytes

            Ling Li, Mizuho Fukunaga-Kalabis, and Meenhard Herlyn

 

3. Isolation, Cultivation, and Application of Human Alveolar Epithelial Cells

            Nicole Daum, Anna Kuehn, Stephanie Hein, Ulrich F. Schaefer, Hanno Huwer, and Claus-Michael Lehr

 

4. Culture of Parathyroid Cells

            Peyman Björklund and Per Hellman

 

5. Functional Analysis of Human Islets of Langerhans Maintained in Culture

            Shanta J. Persaud, Bo Liu, and Peter M. Jones

 

6. Conversion of Non-Endocrine Human Pancreatic Cells to Insulin Producing Cells for Treatment of Diabetes

            Min Zhao and Guocai Huang

 

7. Evaluation of Cytochrome P450 Activities in Human Hepatocytes In Vitro

            María José Gómez-Lechón, Agustín Lahoz, José V.Castell, and María Teresa Donato

 

8. Human Hepatocytes: Isolation, Culture, and Quality Procedures

            Daniel Knobeloch, Sabrina Ehnert, Lilianna Schyschka, Peter Büchler, Michael Schoenberg, Jörg Kleeff, Wolfgang E. Thasler, Natascha C. Nussler, Patricio Godoy, Jan Hengstler, and Andreas K. Nussler

 

9. Preclinical Testing of Virotherapeutics for Primary and Secondary Tumors of the Liver

            Martina Zimmermann, Timo Weiland, Michael Bitzer, and Ulrich M. Lauer

 

10. Isolation and Functional Studies of Human Fetal Gastric Epithelium in Primary Culture

            Pierre Chailler, Jean-François Beaulieu, and Daniel Ménard

 

11. Isolation, Characterization, and Culture of Normal Human Intestinal Crypt and Villus Cells

            Jean-François Beaulieu and Daniel Ménard

 

12. Primary Culture of Human Renal Proximal Tubule Epithelial Cells and Interstitial Fibroblasts

            Claire C. Sharpe and Mark E.C. Dockrell

 

13. Glomerular Epithelial and Mesangial Cell Culture and Characterization

            Heather M. Wilson and Keith N. Stewart

 

14. Isolation and Culture of Human Adipocytes and Adipose Tissue

            Kirstin A. Carswell, Mi-Jeong Lee, and Susan K. Fried

 

15. Primary Culture of Human Adipocyte Precursor Cells: Expansion and Differentiation

            Thomas Skurk and Hans Hauner

 

16. Primary Culture of Ovarian Cells for Research on Cell Interactions in the Hormonal Control of Steroidogenesis

            Jerzy F. Galas

 

17. Human Vascular Smooth Muscle Cell Culture

            Diane Proudfoot and Catherine Shanahan

 

18. Culture of Human Endothelial Cells from Umbilical Veins

            Richard C.M. Siow

 

19. Human Peripheral Blood Mononuclear Cell Culture for Flowcytometric Analysis of Phosphorylated Mitogen-Activated Protein Kinases

            Athanasios Mavropoulos, Daniel Smyk, Eirini I. Rigopoulou, and Dimitrios P. Bogdanos

 

20. Human CD4+CD25^highCD127^low/neg Regulatory T Cells

            Haibin Su, Maria Serena Longhi, Pengyun Wang, Diego Vergani, and Yun Ma

 

21. Human Chondrocyte Cultures as Models of Cartilage-Specific Gene Regulation

            Miguel Otero, Marta Favero, Cecilia Dragomir, Karim El Hachem, Darren A. Plumb, and Mary B. Goldring

 

22. Isolation and Culture of Human Osteoblasts

            Alison Gartland, Robin M.H. Rumney, Jane P. Dillon, and James A. Gallagher

 

23. Human Osteoclast Culture and Phenotypic Characterization

            Ankita Agrawal, James A. Gallagher, and Alison Gartland

 

24. Effects of Temperature Generated from the Holmium:YAG Laser on Human Osteoblasts in Monolayer Tissue Culture

            Moustafa I. Hafez, Anne Sandison, Richard R.H. Coombs, Ian D.McCarthy, and Al-Shymaa M. Hafez

 

25. Laser Microdissection Microscopy: Application to Cell Culture

            Ahlam Mustafa, Cathy Cenayko, Ragai R. Mitry, and Alberto Quaglia

 

26. Automated Adherent Human Cell Culture (Mesenchymal Stem Cells)

            Robert Thomas and Elizabeth Ratcliffe

 

27. Culturing and Differentiating Human Mesenchymal Stem Cells for Biocompatible Scaffolds in Regenerative Medicine

            William R. Otto and Catherine E. Sarraf

Blurb

Human cell culture is not a new topic, but the development of new molecular techniques and reagents which can be used to investigate cell function and the responsible intracellular mechanisms make it a continuing requirement.  This third edition of Human Cell Culture Protocols expands upon the previous editions with current, detailed protocols for the isolation and culture of a range of primary cells from human tissues.  With new chapters on pancreatic cells needed for basic studies on the pathogenesis of diabetes and for their application for islet transplantation, the book also delves into protocols for hepatocytes, skin cells, lung cells, parathyroid cells, gastric cells, renal cells, adipocytes, ovarian cells, bone cells, vascular smooth muscle cells, vascular endothelial cells, regulatory T cells, blood mononuclear cells, as well as new techniques being applied to human cell culture, particularly the use of biocompatible scaffolds to grow cells, the in vitro use of laser microdissection to isolate cells from culture, and automated cell culture. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Human Cell Culture Protocols, Third Edition makes it possible for a worker with basic cell culture training, whether in the fields of cell biology, gene therapy, and cell transplantation, to prepare cell cultures of the specific cell type necessary to forward their vital research.