Hox Genes: Methods and Protocols

ISBN-13: 9781493912414 / Angielski / Twarda / 2014 / 373 str.

Yacine Graba; Rene Rezsohazy

Opis

Hox Genes: Methods and Protocols explores techniques and methodologies which arose from or were successfully applied to the study of Hox genes and Hox proteins, at the intersection of experimental embryology, genetics, biochemistry, physiology, evolutionary biology and other life sciences. This detailed volume begins with a section on discovery and functional analysis of Hox genes and then it continues onward to discuss mode of action and biomedical applications of Hox proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Expert and practical, Hox Genes: Methods and Protocols serves as an ideal guide to researchers striving to move forward in this dynamic and exciting area of study.

Spis treści

Part I: Hox Genes: From Discovery to Functional Analysis

 

1. Discovery and Classification of Homeobox Genes in Animal Genomes

            Ferdinand Marlétaz, Jordi Paps, Ignacio Maeso, and Peter W.H. Holland

 

2. How to Study Hox Gene Expression and Function in Mammalian Oocytes and Early Embryos

            Delphine Paul, Caroline Sauvegarde, René Rezsohazy, and Isabelle Donnay

 

3. Genetic Lineage Tracing Analysis of Anterior Hox Expressing Cells

            Brigitte Laforest, Nicolas Bertrand, and Stéphane Zaffran

 

4. A Genetic Strategy to Obtain P-Gal4 Elements in the Drosophila Hox Genes

            Luis de Navas, David Foronda, Delia del Saz, and Ernesto Sánchez-Herrero

 

5. Hox Complex Analysis Through BAC Recombineering

            Mark Parrish, Youngwook Ahn, Christof Nolte, Bony De Kumar, and Robb Krumlauf

 

6. The Genetics of Murine Hox Loci: Tamere, String, and Panthere to Engineer Chromosome Variants

            Patrick Tschopp and Denis Duboule

 

7. Topological Organization of Drosophila Hox Genes Using DNA Fluorescent In Situ Hybridization

            Frédéric Bantignies and Giacomo Cavalli

 

8. Mining the Cis-Regulatory Elements of Hox Clusters

            Navneet Kaur Matharu and Rakesh K. Mishra

 

9. Functional Analysis of Hox Genes in Zebrafish

            Franck Ladam and Charles G. Sagerström

 

10. Transgenesis in Non-Model Organisms: The Case of Parhyale

            Zacharias Kontarakis and Anastasios Pavlopoulos

 

11. Tissue Specific RNA Isolation in Drosophila Embryos: A Strategy to Analyze Context Dependent Transcriptome Landscapes Using FACS

            Arnaud Defaye and Laurent Perrin

 

12. Hox Transcriptomics in Drosophila Embryos

            Maria Polychronidou and Ingrid Lohmann

 

Part II: Hox Proteins: Mode of Action and Biomedical Applications

 

13. Measuring Hox-DNA Binding by Electrophoretic Mobility Shift Analysis

            Kelly Churion, Ying Liu, Hao-Ching Hsiao, Kathleen S. Matthews, and Sarah E. Bondos

 

14. Chromatin Immunoprecipitation and Chromatin Immunoprecipitation with Massively Parallel Sequencing on Mouse Embryonic Tissue

            Shilu Amin and Nicoletta Bobola

 

15. ChIP for Hox Proteins from Drosophila Imaginal Discs

            Pavan Agrawal and L.S. Shashidhara

 

16. SELEX-seq: A Method for Characterizing the Complete Repertoire of Binding Site Preferences for Transcription Factor Complexes

            Todd R. Riley, Matthew Slattery, Namiko Abe, Chaitanya Rastogi, Richard Mann, and Harmen Bussemaker

 

17. DamID as an Approach to Studying Long Distance Chromatin Interactions

            Fabienne Cléard, François Karch, and Robert K. Maeda

 

18. cgChIP: A Cell Type and Gene Specific Method for Chromatin Analysis

            Marios Agelopoulos, Daniel J. McKay, and Richard S. Mann

 

19. Bimolecular Fluorescence Complementation (BiFC) in Live Drosophila Embryos

            Marilyne Duffraisse, Bruno Hudry, and Samir Merabet

 

20. Hox Protein Interactions: Screening and Network Building

            Isabelle Bergiers, Barbara Lambert, Sarah Daakour, Jean-Claude Twizere, and René Rezsohazy

 

21. Rational Drug Repurposing Using sscMap Analysis in a HOX-TALE Model of Leukemia

            Laura M.J. Kettyle, Fabio G. Liberante, and Alexander Thompson