Host-Pathogen Interactions: Methods and Protocols

ISBN-13: 9781588298867 / Angielski / Twarda / 2008 / 428 str.

Rupp, Steffen

Opis

In recent decades, infectious diseases, once believed to be fairly contained, have become a vital, resurgent field of research. In Host-Pathogen Interactions: Methods and Protocols, top experts examine the relationship between the host and the pathogen, crucial in the outcome of an infection and the establishment of disease or asymptomatic, commensal colonization by organisms. The step-by-step laboratory methods and protocols of this volume study host-pathogen interaction, with a focus on fungal, bacterial and parasitic pathogens, at a molecular level in order to reveal the mechanisms of infection and to identify the vulnerabilities of the pathogen of interest. Written in the highly successful Methods in Molecular Biology series format, the chapters feature brief subject introductions, lists of the necessary materials and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Host-Pathogen Interactions: Methods and Protocols serves as an easy entry point for all those investigating the factors responsible for the pathogenicity of microorganisms."

Recenzje

From the reviews:

"This book provides an overview of laboratory methods used to evaluate the influence of microbial pathogens on host cells. ... This book is written specifically for scientists interested in using new technologies to study host cell reactions to infectious agents. ... This is a good, solid techniques book that would be useful to scientific laboratories studying a variety of infectious agents." (Rebecca T. Horvat, Doody's Review Service, February, 2009)

"This book is an excellent collection of protocols for use in the study of host-microbe interaction. The protocols are detailed and accurate. Obviously this is not a book to sit down and read, but one to be used as a source of information for conducting experimental work. ... The book will be of use to PhD students and postdoctoral scientists working on host-pathogen interactions. Probably each laboratory working in this area would have a copy rather than each individual." (Tim Mitchell, Microbiology Today, July, 2009)

Spis treści

Part I: Bacterial pathogens 1. Introduction: Bacterial Pathogens Christof Hauck 2. Transcription Analyses in the Interaction of Neisseria meningitidis with Mammalian Host Cells Alexandra Schubert-Unkmeir, Anja Schramm-Glück, Matthias Frosch, and Christoph Schoen 3. Surface-exposed adherence molecules of Streptococcus pneumoniae Sven Hammerschmidt 4. Screening of Virulence Traits in Legionella pneumophila and Analysis of the Host Susceptibility to Infection by Using the Dictyostelium Host Model System Olga Schevchuk and Michael Steinert 5. Characterizing Host Receptor Recognition by Individual Bacterial Pathogens Katharina Kuespert and Christof R. Hauck Part II: Fungal pathogens 6. Introduction: Fungal Pathogens Steffen Rupp 7. Isolation and Purification of Antigenic Components of Cryptococcus Karen L. Wozniak and Stuart M. Levitz 8. A Method for Examining Glycans Surface Expression of Yeasts by Flow Cytometry Maria Martínez-Esparza, Aurore Sarazin, Daniel Poulain and Thierry Jouault 9. Human Epithelial Model Systems for the Study of Candida Infections in vitro: Part I. Adhesion to Epithelial Models Kai Sohn and Steffen Rupp 10. Human Epithelial Model Systems for the Study of Candida Infections in vitro: Part II. Histological Methods for Studying Fungal Invasion Rosa Hernandez and Steffen Rupp 11. In vitro Systems for Studying the Interaction of Fungal Pathogens with Primary Cells from the Mammalian Innate Immunity Christelle Bourgeois, Olivia Majer, Ingrid Frohner and Karl Kuchler 12. Experimental Infection of Rodent Mammals for Fungal Virulence Testing Anja Schweizer and Klaus Schröppel 13.Real-time and Semi-quantitative RT-PCR Methods to Analyze Gene Expression Patterns During Aspergillus-host Interactions Jin Woo Bok, Nancy P. Keller and Dimitrios I. Tsitsigiannis 14. Antibody-bases Strategy to Identify C. albicans Genes Expressed during Infections Cornelius J. Clancy, Shaoji Cheng and M. Hong Nguyen 15. Identification of the Candida albicans Immunome Expressed during Systemic Infection by Mass Spectrometry Aida Pitarch, César Nombela and Concha Gil Part III: Parasites 16. Introduction: Parasites Klaus Brehm and Carsten Lüder 17. Axenic in vitro Cultivation of Echinococcus multilocularis Metacestode Vesicles and the Generation of Primary Cell Cultures Markus Spiliotis and Klaus Brehm 18. Culture of Exoerythrocytic Stages of the Malaria Parasites Plasmodium falciparum and P. vivax Liwang Cui, Namtip Trongnipatt, Jetsumon Sattabongkot and Rachanee Udomsangpetch 19. Modulation of Caspase Activation by Toxoplasma gondii Diana Hippe, Andrea Gais, Uwe Groß and Carsten G. K. Lüder Part IV: Host responses 20. Introduction: Host Responses Martin Schaller, Günther Weindl and Bernhard Hube 21. Fungal and Bacterial Killing by Neutrophils David Ermert, Arturo Zychlinsky and Constantin Urban 22. Endothelial Cell Stimulation by Candida albicans Quynh T. Phan and Scott G. Filler 23. Models of Oral and Vaginal Candidiasis Based on in vitro Reconstituted Human Epithelia for the Study of Host-pathogen Interactions Martin Schaller and Günther Weindl 24. Phagocytosis of Candida albicans by RNAi Treated Drosophila S2 Cells Shannon L. Stroschein-Stevenson, Edan Foley, Patrick H. O'Farrell and Alexander D.