Host-Pathogen Interactions: Methods and Protocols

ISBN-13: 9781493976034 / Angielski / Twarda / 2017 / 317 str.

Carlos Medina; Francisco Javier Lopez-Baena

Opis

Divided into several sections, the book delves into animal-pathogen interaction, microbe-microbe interaction, as well as plant-pathogen interaction.

Spis treści

1. Genetic Association Studies in Host-Pathogens Interaction Analysis

            Jose Luis Royo and Luis Miguel Real

 

2. Bacterial Genotyping Methods: From the Basics to Modern

            Margarita M. Ochoa-Díaz, Silvana Daza-Giovannetty, and Doris Gómez-Camargo

 

Part I: Animal-Pathogen Interaction Section

 

3. Real-Time Reverse Transcription PCR as a Tool to Study Virulence Gene Regulation in Bacterial Pathogens

            Gili Aviv and Ohad Gal-Mor

 

4. The Usage of a Bioluminescence Reporter System to Image Promoter Activity During Host Infection

            Gili Aviv and Ohad Gal-Mor

 

5. lacZ Reporter System as a Tool to Study Virulence Gene Regulation in Bacterial Pathogens

            Gili Aviv and Ohad Gal-Mor

 

6. Western Blotting Against Tagged Virulence Determinants to Study Bacterial Pathogenicity

            Gili Aviv and Ohad Gal-Mor

 

7. Molecular Methods to Analyze the Effect of Proteins Expressed by Salmonella During Its Intracellular Stage

            Carlos Medina, Beatriz Mesa-Pereira, Eva Mª Camacho, Amando Flores, and Eduardo Santero

 

8. Organoids as a Model to Study Infectious Disease

            Kristen A. Engevik, Andrea L. Matthis, Marshall H. Montrose, and Eitaro Aihara

 

9. Surface Proteome Biotinylation Combined with Bioinformatic Tools as a Strategy for Predicting Pathogen Interacting Proteins

            Anita Horvatić, Josipa Kuleš, Nicolas Guillemin, Franjo Martinković, Iva Štimac, Vladimir Mrljak, and Mangesh Bhide

 

10. Systems Biology Modeling to Study Pathogen-Host Interactions

            Müberra Fatma Cesur and Saliha Durmuş

 

11. Phage Therapy: Various Perspectives on How to Improve the Art

            Stephen T. Abedon

 

Part II: Microbe-Microbe Interaction Section

 

12. Application of RNA-seq and Bioimaging Methods to Study Microbe-Microbe Interactions and Their Effects on Biofilm Formation and Gene Expression

            Cristina Isabel Amador, Claus Sternberg, and Lars Jelsbak

 

13. Serial Dilution-Based Growth Curves and Growth Curve Synchronization for High-Resolution Time Series of Bacterial Biofilm Growth

            Fernando Govantes

 

14. Detection of Bacterial Quorum Sensing Molecules

            Elke Stein and Adam Schikora

 

Part III: Plant-Pathogen Interaction Section

 

15. Generating Chromosome-Located Transcriptional Fusions to Fluorescent Proteins for Single-Cell Gene Expression Analysis in Pseudomonas syringae

            José S. Rufián, Diego López-Márquez, Nieves López-Pagán, Murray Grant, Javier Ruiz-Albert, and Carmen R. Beuzón

 

16. Introduction of Genetic Material in Ralstonia solanacearum through Natural Transformation and Conjugation

            Anthony Perrier, Patrick Barberis, and Stephane Genin

 

17. In Vitro and In Vivo Secretion/Translocation Assays to Identify Novel Ralstonia solanacearum Type 3 Effectors

            Fabien Lonjon, Nemo Peeters, Stéphane Genin, and Fabienne Vailleau

 

18. Plant Pathogenicity Phenotyping of Ralstonia solanacearum Strains

            Alice Morel, Nemo Peeters, Fabienne Vailleau, Patrick Barberis, Gaofei Jiang, Richard Berthomé, and Alice Guidot

 

19. Methods to Quantify Biotic-Induced Stress in Plants

            Marcel Bach-Pages and Gail M. Preston

 

20. From Sample to Data: Preparing, Obtaining, and Analyzing Images of Plant-Pathogen Interactions Using Confocal Microscopy

            Helen N. Fones and George R. Littlejohn

 

21. Screening of c-di-GMP-Regulated Exopolysaccharides in Host Interacting Bacteria

            Jochen Schmid, Broder Rühmann, Volker Sieber, Lorena Romero-Jiménez, Juan Sanjuán, and Daniel Pérez-Mendoza

 

22. Primary Characterization of Small RNAs in Symbiotic Nitrogen-Fixing Bacteria

            Marta Robledo, Natalia I. García-Tomsig, and José I. Jiménez-Zurdo

 

23. A New, Non-Destructive, Split-Root System for Local and Systemic Plant Responses Studies with Soybean

            Ángeles Hidalgo, José E. Ruiz-Sainz, and José M. Vinardell

 

24. Methods for the Characterization of Plant-Growth Promoting Rhizobacteria

            Md. Motaher Hossain and Farjana Sultana

Blurb

This detailed volume explores numerous multidisciplinary approaches employed to analyze the role of different molecules or strategies used by different guests to survive inside hosts. Divided into several sections, the book delves into animal-pathogen interaction, microbe-microbe interaction, as well as plant-pathogen interaction. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Host-Pathogen Interactions: Methods and Protocols provides many molecular and bioinformatics techniques currently in use at reputed laboratories in countries all around the world, vital for many kinds of host-pathogen interactions studies.