Host-Pathogen Interactions: Methods and Protocols

ISBN-13: 9781071636169 / Angielski

Carlos Medina; Francisco Javier López-Baena

Spis treści

Part I: Introduction

 

1. Host-Pathogen Interaction: Biology and Public Health

            Richard Ponce-Cusi, Leny Bravo, Kevin J. Paez, Joseph A. Pinto, and Nesstor Pilco-Ferreto

 

2. Genetic Association Studies in Host-Pathogen Interaction Analysis

            Marina Laplana, José Luis Royo, and Luis Miguel Real

 

Part II: Viral-Host Interactions

 

3. Live-Cell Fluorescence Imaging for Virus/Host Interactions

            Francesca J. Scribano, Kristen A. Engevik, J. Thomas Gebert, and Joseph M. Hyser

 

4. Construction of a Mycoviral Infectious Clone for Reverse Genetics in Botrytis cinerea

            Laura Córdoba, Ana Ruiz-Padilla, Javier Pardo-Medina, Julio L. Rodríguez-Romero, and María A. Ayllón

 

Part III: Plant-Pathogens Interactions

 

5.  Exopolysaccharide Production and Precipitation Method as a Tool to Study Virulence Factors

            Natalia Mielnichuk, Constanza M. Joya, María A. Monachesi, and Romina P. Bertani

 

6. Virulence-Related Assays for Investigation of the Acidovorax citrulli-Cucurbitaceae Pathosystem

            Francisco Pérez-Montaño, Irene Jiménez-Guerrero, Dafna Tamir-Ariel, and Saul Burdman

 

7. Dual-Fluorescence Chromosome-Located Labeling System for Accurate In Vivo Single-Cell Gene Expression Analysis in Pseudomonas syringae

            Nieves López-Pagán, José S. Rufián, Javier Ruiz-Albert, and Carmen R. Beuzón

 

8. A Robust Method to Perform In Vitro and In Planta Interbacterial Competition Assays: Killing Plant Pathogens by a Potent Biocontrol Agent

            Cristina Civantos, Adrián Ruiz, and Patricia Bernal

 

Part IV: Molecular Methods for Plant-Interacting Bacteria

 

9. Quantification of Mixed-Linkage β-Glucan (MLG) in Bacteria

            Juan Antonio Marchante, Lucía Ruíz-Sáez, Socorro Muñoz, Juan Sanjuán, and Daniel Pérez-Mendoza

 

10. Surface Plasmon Resonance as a Tool to Elucidate the Molecular Determinants of Key Transcriptional Regulators Controlling Rhizobial Lifestyles

            Laura Tomás-Gallardo, Juan J. Cabrera, and Socorro Mesa

 

11. Microscope Subcellular Localization of Plant-Interacting Bacterial Effectors in Animal Cell Cultures

            Irene Jiménez-Guerrero, Francisco Javier López-Baena, and Carlos Medina

 

12. A Workflow for the Functional Characterization of Noncoding RNAs in Legume Symbiotic Bacteria

            Natalia I. García-Tomsig, Sabina K. Guedes-García, and José I. Jiménez-Zurdo

 

13. Methods for Studying Swimming and Surface Motilities in Rhizobia

            Francisco Fuentes-Romero, Cynthia Alías-Villegas, Pilar Navarro-Gómez, Sebastián Acosta-Jurado, Lydia M. Bernabéu-Roda, Virginia Cuéllar, María J. Soto, and José M. Vinardell

 

14. Isolation, Quantification, and Visualization of Extracellular Membrane Vesicles in Rhizobia Under Free-Living Conditions

            Paula Ayala-García, Natalia Moreno-de Castro, Irene Jiménez-Guerrero, Mathias Müsken, Alejandro Arce-Rodríguez, Francisco Pérez-Montaño, and José Manuel Borrero-de Acuña

 

15. Isolation of Rhizobial Extracellular Membrane Vesicles from Bacteroids

            Paula Ayala-García, Irene Jiménez-Guerrero, Mathias Müsken, Francisco Javier Ollero, José Manuel Borrero-De Acuña, and Francisco Pérez-Montaño

 

16. Nod Factor Lipopolysaccharide Purification to Study Nitrogen-Fixing Bacteria Symbiosis with Legumes

            Catherine Jacott, Sara Lozano-Morillo, and Pablo del Cerro

 

17. A Novel System to Selective Tagging of Sinorhizobium fredii Symbiotic Plasmids

            Ana María Cutiño, María del Carmen Sánchez-Aguilar, José Enrique Ruiz-Sáinz, María del Rosario Espuny, Francisco Javier Ollero, and Carlos Medina

 

18. New Inoculation Strategy for Legume Based on Rhizobium-Metabolite Co-Encapsulation

            Adriana B. Cesari, Verónica E. Castilla Marín, Luciana Nieva Muratore, Natalia S. Paulucci, and Marta S. Dardanelli

Blurb

This fully updated volume serves as multidisciplinary compendium of approaches and techniques employed to analyze the role of different molecules, processes, or strategies used by different guests to survive and proliferate in their associations with eukaryotic hosts. Beginning with animal-pathogen interactions, the book then continues with chapters exploring virus-host interactions, plant-microbe interactions, as well as different molecular techniques that were initially applied to non-pathogenic interactions but can be adapted to study other host-pathogen associations. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips for troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Host-Pathogen Interactions: Methods and Protocols, Second Edition contributes to the study of host-pathogen interactions with numerous techniques that can be used in a variety of bacteria.