Host-Fungus Interactions: Methods and Protocols

ISBN-13: 9781493959044 / Angielski / Miękka / 2016 / 597 str.

Alexandra C. Brand; Donna M. MacCallum

Opis

This exploration of the culture and genetic manipulation of primary fungal pathogens, as well as interactions in model systems, includes detailed, lab-ready techniques as well as the key tips and implementation advice necessary to achieve successful results.

Recenzje

From the book reviews:

"The book 'Host-fungus Interactions' assembles methods and protocols for studying gene disruption, modulation of gene expression, host and fungal responses to infection and several model systems to analyze the host-fungus interactions. The book comprises of 8 parts with 42 chapters and an index at the end of the book. ... A useful tool for medical microbiologists, immunologists, geneticists, biotechnologists and students studying pharmacology and pathogenicity testing." (Melvina D'souza and Kevin D. Hyde, Fungal Diversity, December, 2014)

Spis treści

Part I: Gene Disruption

 

1. Gene Deletion in Candida albicans Wild-Type Strains Using the SAT1-Flipping Strategy

            Christoph Sasse and Joachim Morschhäuser

 

2. Mini-Blaster-Mediated Targeted Gene Disruption and Marker Complementation in Candida albicans

            Shantanu Ganguly and Aaron P. Mitchell

 

3. Rapid Detection of Aneuploidy Following the Generation of Mutants in Candida albicans

            Megan D. Lenardon and André Nantel

 

4. Agrobacterium-Mediated Insertional Mutagenesis in Histoplasma capsulatumi

            Olga Zemska and Chad A. Rappleye

 

5. Targeted Gene Disruption in Cryptococcus neoformans Using Double Joint PCR with Split Dominant Selectable Markers

            Min Su Kim, Seo-Young Kim, Kwang-Woo Jung, and Yong-Sun Bahn

 

6. Multiple Gene Deletion in Cryptococcus neoformans Using the Cre--lox System

            Lorina G. Bakerand and Jennifer K. Lodge

 

7. Gene Disruption in Aspergillus fumigatus Using a PCR-Based Strategy and In Vivo Recombination in Yeast

            Iran Malavazi and Gustavo Henrique Goldman

 

8. Targeted Gene Deletion in Aspergillus fumigatus Using the Hygromycin-Resistance Split-Marker Approach

            Fabrice N. Gravelat, David S. Askew, and Donald C. Sheppard

 

9. Gene Disruption in Coccidioides Using Hygromycin or Phleomycin Resistance Markers

            Chiung-Yu Hung, Hua Zhang Wise, and Garry T. Cole

 

Part II: Modulation of Gene Expression: RNAi Gene Knockdown

 

10. RNAi-Based Gene Silencing Using a GFP Sentinel System in Histoplasma capsulatum

            Brian H. Youseff and Chad A. Rappleye

 

11. RNA Interference in Cryptococcus neoformans

            Michael L. Skowyra and Tamara L. Doering

 

12. Gene Knock-Down in Paracoccidioides brasiliensis Using Antisense RNA

            João F. Menino, Agostinho J. Almeida, and Fernando Rodrigues

 

Part III: Modulation of Gene Expression: Regulatable Promoters

 

13. Tetracycline-Inducible Gene Expression in Candida albicans

            Michael Weyler and Joachim Morschhäuser

 

14. Galactose-Inducible Promoters in Cryptococcus neoformans var. grubii

            Lorina G.  Baker and Jennifer K. Lodge

 

15. Modular Gene Over-Expression Strategies for Candida albicans

            Vitor Cabral, Murielle Chauvel, Arnaud Firon, Mélanie Legrand, Audrey Nesseir, Sophie Bachellier-Bassi, Yogesh Chaudhari, Carol Munro, and Christophe d’Enfert

 

Part IV: Responses to Infection In Vivo

 

16. Interactions Between Macrophages and Cell-Wall Oligosaccharides of Candida albicans

            Héctor M. Mora-Montes, Christopher McKenzie, Judith M. Bain, Leanne E. Lewis, Lars P. Erwig, and Neil A.R. Gow

 

17. Murine Bone Marrow-Derived Dendritic Cells and T Cell Activation by Candida albicans

            Joanne Gibson, Neil A.R. Gow, and Simon Y.C. Wong

 

18. Phagocytosis and Intracellular Killing of Candida albicans by Murine Polymorphonuclear Neutrophils

            Alieke G. Vonk, Mihai G. Netea, and Bart Jan Kullberg

 

19. Human Oral Keratinocytes: A Model System to Analyze Host-Pathogen Interactions

            Torsten Wöllert, Christiane Rollenhagen, George M. Langford, and Paula Sundstrom

 

20. Simple Assays for Measuring Innate Interactions with Fungi

            Ann M. Kerrigan, Maria da Glória Teixeira de Sousa, and Gordon D. Brown

 

21. Binding and Uptake of Candida albicans by Human Monocyte-Derived Dendritic Cells

            Annemiek B. van Spriel and Alessandra Cambi

 

22. Immune Responses to Candida albicans in Models of In Vitro Reconstituted Human Oral Epithelium

            Jeanette Wagener, Daniela Mailänder-Sanchez, and Martin Schaller

 

23. Analysis of Host-Cell Responses by Immunoblotting, ELISA, and Real-Time PCR

            David L. Moyes and Julian R. Naglik

 

24. In Vitro Model of Invasive Pulmonary Aspergillosis in the Human Alveolus

            Lea Gregson, William W. Hope, and Susan J. Howard

 

25. Biofilm Formation Studies in Microtiter Plate Format

            Marta Riera, Emilia Moreno-Ruiz, Sophie Goyard, Christophe d’Enfert, and Guilhem Janbon

 

Part V: Responses to Infection In Vivo: Fungal Response

 

26. Transcript Profiling Using ESTs from Paracoccidioides brasiliensis in Models of

Infection

            Alexandre Melo Bailão, Maristela Pereira, Silvia Maria Salem-Izacc, Clayton Luiz Borges, and Célia Maria de Almeida Soares

 

27. Laser Capture Microdissection of Candida albicans from Host Tissue

            Caroline Westwater and David A. Schofield

 

28. Isolation and Amplification of Fungal RNA for Microarray Analysis from Host Samples

            Anja Lüttich, Sascha Brunke, and Bernhard Hube

 

Part VI: Responses to Infection In Vivo: Host Response

 

29. Cytokine Measurement Using Cytometric Bead Arrays (CBA)

            Luis Castillo and Donna M. MacCallum

 

30. Transcript Profiling of the Murine Immune Response to Invasive Aspergillosis

            Zaneeta Dhesi, Susanne Herbst, and Darius Armstrong-James

 

Part VII: Responses to Infection In Vivo: Model Systems for Host-Fungal Interactions

 

31. Caenorhabditis elegans: A Nematode Infection Model for Pathogenic Fungi

Maged Muhammed, Jeffrey J. Coleman, and Eleftherios Mylonakis

 

32. Drosophila melanogaster as a Model Organism for Invasive Aspergillosis

Michail S. Lionakis and Dimitrios P. Kontoyiannis

 

33. Galleria mellonella as a Model for Fungal Pathogenicity Testing

John Fallon, Judy Kelly, and Kevin Kavanagh

 

34. Embryonated Chicken Eggs as Alternative Infection Model for Pathogenic Fungi

Ilse D. Jacobsen, Katharina Große, and Bernhard Hube

 

Part VIII: Responses to Infection In Vivo: Mammalian Hosts

 

35. Mouse Intravenous Challenge Models and Applications

Donna M. MacCallum

 

36. A Nebulized Intra-Tracheal Rat Model of Invasive Pulmonary Aspergillosis

Guillaume Desoubeaux and Jacques Chandenier

 

37. Invasive Models of Histoplasmosis

A. George Smulian

 

38. Murine Model of Concurrent Oral and Vaginal Candida albicans Colonization

Durdana Rahman, Mukesh Mistry, Selvam Thavaraj, Julian R. Naglik, and Stephen J. Challacombe

 

39. A Luciferase Reporter for Gene Expression Studies and Dynamic Imaging of Superficial Candida albicans Infections

Donatella Pietrella, Brice Enjalbert, Ute Zeidler, Sadri Znaidi, Anna Rachini, Anna Vecchiarelli, and Christophe d’Enfert

 

40. Modeling of Fungal Biofilms Using a Rat Central-Vein Catheter

Jeniel E. Nett, Karen Marchillo, and David R. Andes

 

41. Orogastrointestinal Model of Mucosal and Disseminated Candidiasis

Karl V. Clemons and David A. Stevens

 

42. A Non-Lethal Murine Cutaneous Model of Invasive Aspergillosis

Ronen Ben-Ami and Dimitrios Kontoyiannis

Blurb

Microbiologists, medical mycologists, immunologists, and biochemists are increasingly working together to focus on the processes involved in the progression and treatment of fungal disease.  Host-Fungus Interactions: Methods and Protocols is designed for research scientists who are involved in this work and interested in undertaking new or comparative studies of interactions between the mammalian host and clinically important fungal pathogens. Aiming to combine approaches for reverse genetics in pathogenic fungi with methods for their application in in vitro and in vivo models of disease, the book includes methods for the culture and genetic manipulation of the primary fungal pathogens and the opportunistic pathogens, as well as methods for investigating host-fungus interactions in model systems.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Comprehensive and practical, Host-Fungus Interactions: Methods and Protocols describes available molecular methods and fungal infection models in great detail in order to encourage researchers to try new approaches to investigating host-fungus interactions with added levels of confidence.