Host-Fungal Interactions: Methods and Protocols

ISBN-13: 9781071611814 / Angielski / Twarda / 2021 / 242 str.

Elaine Bignell

Spis treści

1. Diminished Expression Alleles for Analysis of Virulence Traits and Genetic Interactions in Candida albicans

Carol A. Woolford and Aaron P. Mitchell

 

2. Serological Proteome Analysis for the Characterisation of Secreted Fungal Protein Antigens

Juliane Macheleidt and Olaf Kniemeyer

 

3. Identification of Host Receptors for Fungi Using Whole Cell Affinity Purification

Quynh T. Phan and Scott G. Filler

 

4. CRISPR-Cas9 Mediated Gene Silencing in Cultured Human Epithelia Sara Gago, Nicola LD Overton, and Paul Bowyer

 

5. Analysis of Epithelial Cell Responses to Microbial Pathogens

Spyridoula Nikou, Jemima Ho, Olivia Hepworth, Nicole Ponde, Ruth Dickenson, Jonathan P. Richardson, and Julian R. Naglik

 

6. Single-cell Analysis of Fungal Uptake in Cultured Airway Epithelial Cells Using Differential Fluorescent Staining and Imaging Flow Cytometry

Margherita Bertuzzi and Gareth J. Howell

 

7.

 

8. Fungal Bioreporters to Monitor Outcomes of Aspergillus – Host Cell Interactions

Neta Shlezinger, Jeffrey Scott Fites, Bruce S. Klein, and Tobias M. Hohl

 

9. Candida albicans Interaction with Oral Epithelial Cells: Adhesion, Invasion, and Damage Assays

Selene Mogavero and Bernhard Hube

 

10. Experimental Evolution of Candida by Serial Passaging in Host Cells

Katja Graf, Bernhard Hube, and Sascha Brunke

 

11. Quantifying Receptor-mediated Phagocytosis and Inflammatory Responses to Fungi in Immune Cells

Patawee Asamaphan, Gordon D. Brown, and Janet Willment

 

12. Measuring In Vivo Neutrophil Trafficking Responses during Fungal Infection Using Mixed Bone Marrow Chimeras

Rebecca A. Drummond and Michail S. Lionakis

 

13. Detection of Low Oxygen Microenvironments in a Murine Model of Invasive Pulmonary Aspergillosis Using Pimonidazole

Authors:

 

14. Murine Models of Hematopoietic Cell Transplantation to Investigate Fungal Infections

Jorge Amich

15. A Murine Model for Chronic A. fumigatus Airway Infections

Benjamin Ralph and Donald C. Sheppard

 

16. Automated Quantitative Analysis of Airway Epithelial Cell Detachment upon Fungal Challenge

Sayema Rahman, Darren D. Thomson^, and Margherita Bertuzzi

 

Blurb

This volume provides readers with essential protocols for dissecting the host-fungus interaction, and engages researchers in study of mammalian disease. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Host-Fungal Interactions: Methods and Protocols aims to ensure successful results in the further study of this vital field.