Hepatocyte Transplantation: Methods and Protocols

ISBN-13: 9781588298836 / Angielski / Twarda / 2009 / 231 str.

Anil Dhawan; Robin D. Hughes

Opis

Due to its efficacy in animal models, cellular therapy using human hepatocytes is being evaluated worldwide as an alternative to organ transplantation in patients with liver-based metabolic disease and acute liver failure. In Hepatocyte Transplantation: Methods and Protocols, an international panel of experts provide up-to-date laboratory and clinical techniques covering the many key areas necessary for successful transplantation, such as cryopreservation, quality assurance, detection of cell engraftment, and the future of the field with the development of foetal hepatoblasts and stem cell derived hepatocytes. Written in the highly successful Methods in Molecular Biology series format, the chapters in this volume present brief introductions to the material, lists of the necessary materials and reagents, readily reproducible, step-by-step laboratory protocols, and Notes sections which highlight tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Hepatocyte Transplantation: Methods and Protocols is an ideal guide for researchers setting out in the rapidly progressing field of hepatocyte transplantation, as well as those who already have experience with this new therapy for liver disease.

Spis treści

1. Human Hepatocyte Transplantation Overview

Juliana Puppi and Anil Dhawan

2. Isolation of Human Hepatocytes

Ragai R. Mitry

3. An Optimised Method for Cryopreservation of Human Hepatocytes

Claire Terry and Robin D. Hughes

4. Liver Cell Culture Techniques

José V. Castell and Maria José Gómez-Lechón

5. In vitro Assays for Induction of Drug Metabolism

Brian G. Lake, Roger J. Price, Amanda M. Giddings and David G. Walters

6. Hepatocyte Apoptosis

Mustapha Najimi, Françoise Smets and Etienne Sokal

7. Small Animal Models of Hepatocyte Transplantation

Jurgen Seppen, Ebtisam El Filali and Ronald Oude Elferink

8. Hepatocyte Transplantation Techniques: Large Animal Models

Anne Weber, Marie-Thérèse Groyer-Picard and Ibrahim Dagher

9. Cell Transplant Techniques: Engraftment Detection of Cells

Robert A. Fisher and Valeria R. Mas

10. Hepatic Preconditioning for Transplanted Cell Engraftment and Proliferation

Yao-Ming Wu and Sanjeev Gupta

11. Ex vivo Gene Transfer into Hepatocytes

Xia Wang, Prashant Mani, Debi P. Sarkar, Namita Roy-Chowdhury and Jayanta Roy-Chowdhury

12. Sources of Adult Hepatic Stem Cells: Haematopoietic

Rosemary Jeffery, Richard Poulsom and Malcolm R. Alison

13. Production of Hepatocyte-like Cells from Human Amnion

Toshio Miki, Fabio Marongiu, Ewa C.S. Ellis, Ken Dorko, Keitaro Mitamura, Aarati Ranade, Roberto Gramignoli and Stephen C. Strom

14. Generation of Hepatocytes from Human Embryonic Stem Cells

Niloufar Safinia and Stephen L. Minger

15. Isolation, In vitro Cultivation and Characterisation of Foetal Liver Cells

Yue Wu, Chetan C. Shatapathy and Stephen L. Minger

16. Human Intrahepatic Biliary Epithelial Cell Lineages: Studies in vitro

Ruth Joplin and Stivelia Kachilele

17. Liver Cell Labelling with MRI Contrast Agents

Michel Modo, Thomas J. Meade and Ragai R. Mitry

18. Microbiological Monitoring of Hepatocyte Isolation in the GMP Laboratory

Sharon C. Lehec