Hepatic Stem Cells: Methods and Protocols

ISBN-13: 9781493989607 / Angielski / Twarda / 2018 / 248 str.

Naoki Tanimizu

Spis treści

Preface…

Table of Contents…

Contributing Authors…

Part I Isolation of Progenitor Cells

1. Long-Term Culture of Mouse Fetal Hepatic Stem/Progenitor Cells

Atsunori Tsuchiya and Shuji Terai

2. Isolation of Bipotential Liver Progenitor Cells from Neonatal Mouse Liver

Naoki Tanimizu

3. Identification and Isolation of Clonogenic Cholangiocyte in Mouse

Bin Li, Craig Dorrell, Pamela S. Canady, and Leslie Wakefield

4. Isolation and Expansion of Rat Hepatocytic Progenitor Cells

   Junichi Kino, Norihisa Ichinohe, Masayuki Ishii, and Toshihiro Mitaka

Part II Characterization of Liver Progenitors In Vivo

5. Genetic Lineage Tracing of Biliary Epithelial Cells

Teresa Rubio-Tomás, Beatriz Aguilar-Bravo, and Pau Sancho-Bru

6. Specific Labeling and Lineage Tracing of Periportal Hepatocytes Using Two-Step Genetic Recombination

Nicola de Prisco, Eleanor Stout, and Joan Fong-Burgada

7. Analysis for the Heterogeneity of Liver Progenitor Cells 

Kenji Kamimoto

8. Chemical Screening Using a Zebrafish Model for Liver Progenitor Cell-Driven Liver Regeneration

Sungjin Ko and Donghun Shin

Part III Generation of Hepatocytes, Cholangiocytes, and Their Progenitors

9. Conversion of Fibroblasts to Hepatocytes In Vitro 

Pengyu Huang, Lulu Sun, Ludi Zhang, and Lijian Hui

10. Conversion of Fibroblasts to Hepatocyte-Like Cells In Vivo 

Guangqi Song, Qinggong Yuan, Zhen Dai, Hsin-Chieh Tsay, Xizhong Shen, Michael Ott, and Amar Deep Sharma

11. Chemically Induced Liver Progenitors (CLiPs): A Novel Cell Source for Hepatocytes and Biliary Epithelial Cells

Takeshi Katsuda and Takahiro Ochiya

12. Induction of Functional Hepatocytes from Human iPSCs

Taketomo Kido and Yuta Koui

13. Culture System of Bile Duct-Like Cystic Structures Derived from Human Inducible Pluripotent Stem Cells 

Akihide Kamiya, Kazuya Anzai, Kota Tsuruya, and Hiromi Chikada

Part IV Reconstitution of Liver Tissue Structures

14. Generation of Hepatic Tissue Structures Using Multicellular Spheroid Culture

Fumiya Tao, Hirotaka Mihara, and Nobuhiko Kojima

15. Reconstruction of Hepatic Tissue Structures Using Interstitial Flow in a Microfluidic Device 

Ryo Sudo

16. Generation of Hepatic Organoids with Biliary Structures

Takeshi Katsuda, Takahiro Ochiya, and Yasuyuki Sakai

17. Analysis for Remodeling Hepatic Tissue Structures in 3D during Regeneration

Kota Kaneko

Part V Liver Injury Models

18. Canine Liver Fibrosis Model to Assess the Functions of Infused Autologous Bone Marrow-Derived Cells 

Taro Takami, Kenji Tani, Yasuho Taura, and Isao Sakaida

19. A Rodent Model for Cell Transplantation of Hepatic Progenitor Cells

Sei Kakinuma and Akihide Kamiya

20. Mouse Model for Hepatocellular Carcinoma and Cholangiocarcinoma Originated from Mature Hepatocytes

Masahiro Yamamoto, Bing Xin, and Yuji Nishikawa

21. Mouse Model for Cholangiocarcinoma from Peribiliary Glands 

Hayato Nakagawa, Nobumi Suzuki, and Kazuhiko Koike 

Blurb

This volume looks at the liver’s epithelial cells—hepatocytes and cholangiocytes—and their progenitors. This book is divided into five parts: isolation of progenitor cells; characterization of liver progenitors in vivo; generation of hepatocytes, cholangiocytes, and their progenitors; reconstitution of liver tissue structures; and liver injury models. The chapters in this book cover topics such as expansion of bipotential liver stem/progenitor cells (LPCs) from fetal and neonatal liver; identifying progenitor cells involved in liver regeneration in vivo; methods for generating hepatocytes and cholangiocytes from multiple cellular sources; 3D tissue structures ex vivo; and resolving hepatic fibrosis by bone marrow transplantation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Hepatic Stem Cells: Methods and Protocols is a valuable resource to help researchers understand the current theories used to study hepatic stem/progenitor cells, and aid them in performing experiments related to liver biology and pathophysiology.