Hepatic Stellate Cells: Methods and Protocols

ISBN-13: 9781071632062 / Angielski

Ralf Weiskirchen; Scott L. Friedman

Opis

This detailed volume explores hepatic stellate cells, which have become a spotlight of liver cell biology due to their pleiotropic functions that extend well beyond extracellular matrix production to pivotal roles in hepatic homeostasis, immunity, and metabolism. The book features methods to isolate, evaluate, and manipulate this cell type in an effort to elucidate hepatic biology and establish prospects for treating disease. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Hepatic Stellate Cells: Methods and Protocols serves as an ideal aid to help researchers accelerate the outstanding science that has steadily unveiled the mysteries of stellate cell biology and their role in disease.

Spis treści

1. Isolation, Purification, and Culture of Primary Murine Hepatic Stellate Cells: An Update

            Steffen K. Meurer, Sabine Weiskirchen, Carmen G. Tag, and Ralf Weiskirchen

 

2. Differentiation of Hepatic Stellate Cells from Pluripotent Stem Cells

            Raquel A. Martínez García de la Torre and Pau Sancho-Bru

 

3. Testing Cell Migration, Invasion, Proliferation, and Apoptosis in Hepatic Stellate Cells

            Miriam Wankell and Lionel Hebbard

 

4. Phalloidin Staining for F-Actin in Hepatic Stellate Cells

            Sarah K. Schröder, Carmen G. Tag, Sabine Weiskirchen, and Ralf Weiskirchen

 

5. Retinyl Ester Analysis by Orbitrap Mass Spectrometry

            Jeroen W.A. Jansen, Maya W. Haaker, Esther A. Zaal, and J. Bernd Helms

 

6. Studying Hepatic Stellate Cell Senescence

            Sandra A. Serna-Salas, Abel A. Soto-Gámez, Zongmei Wu, Myrthe Klaver, and Han Moshage

 

7. Isolation of Hepatic Stellate Cells and Lymphocytes for Co-Culture Systems

            Hee-Hoon Kim, Kyurae Kim, Song Hwa Hong, and Won-Il Jeong

 

8. Working with Immortalized Hepatic Stellate Cell Lines

            Scott Friedman and Ralf Weiskirchen

 

9. Induction of Obstructive Cholestasis in Mice

            Ralf Weiskirchen, Sabine Weiskirchen, Carmen G. Tag, and Steffen K. Meurer

 

10. Mouse Models for Hepatic Stellate Cell Activation and Liver Fibrosis Initiation

            Yulia A. Nevzorova, Ralf Weiskirchen, and Christian Liedtke

 

11. Generation and Culture of Primary Mouse Hepatocyte-HSC Spheroids

            Inge Mannaerts, Nathalie Eysackers, and Leo A. van Grunsven

 

12. Hepatic Stellate Cell Depletion and Genetic Manipulation

            Qiuyan Sun and Robert F. Schwabe

 

13. Human Hepatic Stellate Cells: Isolation and Characterization

            Xiao Liu, David A. Brenner, and Tatiana Kisseleva

 

14. Decellularization of Human Liver to Generate Native Extracellular Matrix for Use in Automated Functional Assays with Stellate Cells

            Emma L. Shepherd, Ellie Northall, Pantelitsa Papakyriacou, Karolina Safranska, Karen Sorensen, and Patricia F. Lalor

 

15. Multiplex Immunostaining to Spatially Resolve the Cellular Landscape in Human and Mouse Livers

            Adrien Guillot, Marlene Sophia Kohlhepp, and Frank Tacke

 

16. Single Cell Secretome Analyses of Hepatic Stellate Cells: Aiming for Single Cell Phenomics

            Richell Booijink, Leon Terstappen, and Ruchi Bansal

 

17. Hepatic Stellate Cells Targeting Using Peptide-Modified Biologicals

            Ruchi Bansal and Klaas Poelstra

 

18. Experimental Workflow for Preclinical Studies of Human Antifibrotic Therapies

            Lien Reolizo, Michitaka Matsuda, and Ekihiro Seki