Hemostasis and Thrombosis: Methods and Protocols

ISBN-13: 9781493971947 / Angielski / Twarda / 2017 / 565 str.

Emmanuel J. Favaloro; Giuseppe Lippi

Spis treści

Part I: Introductory Chapters

 

1. Overview of Hemostasis and Thrombosis and Contribution of Laboratory Testing to Diagnosis and Management of Hemostasis and Thrombosis Disorders

            Roslyn A. Bonar, Giuseppe Lippi, and Emmanuel J. Favaloro

 

2. Preanalytical Issues in Hemostasis and Thrombosis Testing

            Giuseppe Lippi and Emmanuel J. Favaloro

 

3. The Interface between Immunotransfusion and Hemostasis and Thrombosis Testing

            Massimo Franchini

 

Part II: Routine Coagulation Assays

 

4. Optimizing the Verification of Mean Normal Prothrombin Time (MNPT) and International Sensitivity Index (ISI) for Accurate Conversion of Prothrombin Time (PT) to International Normalized Ratio (INR)

            Emmanuel J. Favaloro

 

5. Performance of Activated Partial Thromboplastin Time (APTT): Determining Reagent Sensitivity to Factor Deficiencies, Heparin, and Lupus Anticoagulants

            Geoffrey Kershaw

 

6. Performance and Interpretation of Mixing Tests in Coagulation

            Geoffrey Kershaw

 

7. D-Dimer Testing: Laboratory Aspects and Current Issues

            Jecko Thachil, Giuseppe Lippi, and Emmanuel J. Favaloro

 

8. Determination of Fibrinogen and Thrombin Time (TT)

            Anetta Undas

 

Part III: Thrombophilia-Related Chapters

 

9. An Overview of Thrombophilia and Associated Laboratory Testing

            Martina Montagnana, Giuseppe Lippi, and Elisa Danese

 

10. Laboratory Testing for Activated Protein C Resistance (APCR)

            Soma Mohammed and Emmanuel J. Favaloro

 

11. Assessment of Hereditary Thrombophilia: Performance of Protein C (PC) Testing

            Richard A. Marlar and Jana N. Gausman

 

12. Assessment of Hereditary Thrombophilia: Performance of Protein S (PS) Testing

            Richard A. Marlar and Jana N. Gausman

 

13. Assessment of Hereditary Thrombophilia: Performance of Antithrombin (AT) Testing

            Jana N. Gausman and Richard A. Marlar

 

14. Lupus Anticoagulant Testing: Diluted Russell Viper Venom Time (dRVVT)

            Vittorio Pengo, Elisa Bison, Alessandra Banzato, Giacomo Zoppellaro, Seena Padayattil Jose, and Gentian Denas

 

15. Lupus Anticoagulant Testing: Activated Partial Thromboplastin Time (APTT) and Silica Clotting Time (SCT)

            Armando Tripodi and Veena Chantarangkul

 

16. Solid Phase Immunoassay for the Detection of Anticardiolipin Antibodies

            Rohan Willis, Elizabeth Papalardo, and E. Nigel Harris

 

17. Solid Phase Immunoassay for the Detection of Anti-β2 Glycoprotein I Antibodies

            Rohan Willis, Elizabeth Papalardo, and E. Nigel Harris

 

18. Measuring Direct Oral Anticoagulants

            Robert C. Gosselin and Jonathan Douxfils

 

19. Laboratory Testing Protocols for Heparin-Induced Thrombocytopenia (HIT) Testing

            Kun Kan Edwin Lau, Soma Mohammed, Leonardo Pasalic, and Emmanuel J. Favaloro

 

Part IV: Bleeding Disorders

 

20. One-Stage Factor VIII Assays

            Elizabeth Duncan and Susan Rodgers

 

21. Chromogenic Factor VIII Assays for Improved Diagnosis of Hemophilia A

            Susan Rodgers and Elizabeth Duncan

 

22. Assessment of Factor XIII

            László Muszbek, Éva Katona, and Adrienne Kerényi

 

23. Detection and Measurement of Factor Inhibitors

            Geoffrey Kershaw

 

24. Platelet Function Tests: Preanalytical Variables, Clinical Utility, Advantages and Disadvantages

            Anne-Mette Hvas and Erik Lerkevang Grove

 

25. Platelet Function Analyzed by Light Transmission Aggregometry

            Anne-Mette Hvas and Emmanuel J. Favaloro

 

26. Whole Blood Platelet Aggregometry

            George A. Fritsma and David L. McGlasson

 

27. Assessment of Platelet Function in Whole Blood by Flow Cytometry

            Leonardo Pasalic

 

28. Flow Cytometry Protocols for Assessment of Platelet Function in Whole Blood

            Leonardo Pasalic, Gabrielle J. Pennings, David Connor,  Heather Campbell, Leonard Kritharides, and Vivien M. Chen

 

29. Diagnosis or Exclusion of Von Willebrand Disease Using Laboratory Testing

            Emmanuel J. Favaloro

 

30. Laboratory Testing for Von Willebrand Factor Antigen (VWF:Ag)

            Emmanuel J. Favaloro, Soma Mohammed, and Jürgen Patzke

 

31. Laboratory Testing for Von Willebrand Factor Collagen Binding (VWF:CB)

            Emmanuel J. Favaloro and Soma Mohammed

 

32. Laboratory Testing for Von Willebrand Factor Ristocetin Cofactor (VWF:RCo)

            Soma Mohammed and Emmanuel J. Favaloro

 

33. Laboratory Testing for Von Willebrand Factor Activity by Glycoprotein Ib Binding Assays (VWF:GPIb)

            Jürgen Patzke and Emmanuel J. Favaloro

 

34. Laboratory Testing for Von Willebrand Factor Factor VIII Binding (for 2N VWD)

            Soma Mohammed and Emmanuel J. Favaloro

 

35. Ristocetin-Induced Platelet Aggregation (RIPA) and RIPA Mixing Studies

            Juan Pablo Frontroth and Emmanuel J. Favaloro

 

36. Laboratory Testing for Von Willebrand Factor Multimers

            Susan Oliver, Kun Kan Edwin Lau, Kent Chapman, and Emmanuel J. Favaloro

 

Part V: Global Assays and Post-Analytical Issues

 

37. Thrombin Generation Assays (TGA)

            Gian Luca Salvagno and Erik Berntorp

38. The Overall Haemostatic Potential (OHP) Assay

            Jennifer Curnow

 

39. Thromboelastography (TEG)

            Maha Othman and Harmanpreet Kaur

 

40. Post-Analytical Issues in Hemostasis and Thrombosis Testing

            Emmanuel J. Favaloro and Giuseppe Lippi

Blurb

This collection thoroughly explores the dynamic and ever-developing field of hemostasis and thrombosis diagnostics and research. After an introductory section covering the basics and preanalytical issues, the book continues with in-depth sections that explore how to get the best outcomes from routine coagulation and specialized hemostasis assays, thrombophilia-related techniques, investigations into bleeding disorders, as well as performance of global assays of hemostasis, and finally post-analytical issues in hemostasis and thrombosis testing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Comprehensive and practical, Hemostasis and Thrombosis: Methods and Protocols serves as an ideal resource for researchers and diagnostic laboratories seeking expert guidance and working to identify the best methodologies to pursue hemostasis and thrombosis testing.