Hemostasis and Thrombosis: Methods and Protocols

ISBN-13: 9781071631744 / Angielski / Twarda / 2023 / 855 str.

Emmanuel J. Favaloro; Robert C. Gosselin

Opis

This second edition volume expands on the previous edition with updates about the latest state-of-the-art techniques used in leading hemostasis and thrombosis laboratories for diagnosis and exclusion of hemorrhagic and thrombotic diseases. The chapters in this book are organized into seven parts. Part One provides a general overview on hemostasis and thrombosis, preanalytical issues in testing, and routine hemostasis assays. Part Two covers laboratory testing for thrombophilia, including reviews for activated protein C resistance, protein C, lupus anticoagulant testing, and antiphospholipid antibodies. Part Three addresses monitoring continuous anticoagulant infusions and measuring the effects of oral anti-thrombotic therapy. Part Four talks about heparin induced thrombocytopenia and vaccine induced immune thrombotic thrombocytopenia. Part Five and Six cover ADAMTS13 activity testing and new information on bleeding disorders such as chromogenic factor VIII assays, measurement of emicizumab, and treatment of hemophilia A and B. Finally, Part Seven discusses global assays, research applications, and postanalytical considerations. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Hemostasis and Thrombosis: Methods and Protocols, Second Edition is a valuable resource for scientists and researchers struggling to identify the appropriate methods for hemostasis and thrombosis testing, or who seek additional expert guidance on such testing.

Spis treści

Dedication…

Preface…

Table of Contents…

Contributing Authors…

Part I Introductory Chapters and Routine Coagulation Assays

1. Hemostasis and Thrombosis: An Overview Focusing on Associated Laboratory Testing to Diagnose and Help Manage Related Disorders

Emmanuel J. Favaloro, Robert C. Gosselin, Leonardo Pasalic, and Giuseppe Lippi

2. Preanalytical Variables in Hemostasis Testing

Robert C. Gosselin

3. Automation in the Thrombosis and Hemostasis Laboratory

Eric Salazar and Russell Higgins

4. Ultracentrifugation for Coagulation Testing

Robert C. Gosselin

5. Harmonization of Hemostasis Testing Across a Large Laboratory Network: An Example from Australia

Emmanuel J. Favaloro, Soma Mohammed, Ronny Vong, and Leonardo Pasalic

6. Auto-Validation of Routine Coagulation/Hemostasis Assays with Reflex Testing of Abnormal Test Results

Emmanuel J. Favaloro, Soma Mohammed, Violeta Ule Priebbenow, Ronny Vong, and Leonardo Pasalic

7. Laboratory Testing of Hemostasis in Pregnancy: A Brief Overview

Maha Othman and Anushka Pradhan

8. Protocols for D-Dimer Measurements for Aid to Diagnosis or Exclusion of Venous Thromboembolism

Robert C. Gosselin

9. Performance and Interpretation of Clot Waveform Analysis

Maria Filomena Ruberto, Francesco Marongiu, and Doris Barcellona

Part II Thrombophilia Related Chapters

10. Laboratory Evaluation of Thrombophilia

Richard A. Marlar

11. Laboratory Testing for Activated Protein C Resistance (APCR): An Update

Emmanuel J. Favaloro, Soma Mohammed, Ronny Vong, and Leonardo Pasalic

12. Evaluation of Activated Protein C Resistance using Thrombin Generation Test

Jonathan Douxfils, Céline Bouvy, and Laure Morimont

13. Performance of Chromogenic Protein C (PC) Testing

Rebecca Adams, Robyn Coleman, and Timothy Stanton

14. Testing for Factor V Leiden (FVL) and Prothrombin G20210A Genetic Variants

Tadej Pajič

15. An Overview of Laboratory Testing for Antiphospholipid Antibodies

Emmanuel J. Favaloro and Leonardo Pasalic

16. Lupus Anticoagulant Testing: Taipan Snake Venom Time with Ecarin Time as Confirmatory Test

Gary W. Moore

17. Lupus Anticoagulant Testing: Dilute Prothrombin Time (dPT)

Gary W. Moore

18. An Acute Need Inspiration: Autoneutralization of Lupus Anticoagulants in Affected Prothrombin Times (PT) and Activated Partial Thromboplastin Times (APTT)

Robert C. Gosselin

19. Antiphospholipid Antibody Testing for Anti-Cardiolipin and Anti-2Glycoprotein I Antibodies using Chemiluminescence-Based Panels

Emmanuel J. Favaloro, Soma Mohammed, Ronny Vong, and Leonardo Pasalic

20. Laboratory Testing for Non-Criteria Antiphospholipid Antibodies-Antiphosphatidylserine/Prothrombin Antibodies (aPS/PT)

Katrien M.J. Devreese

21. Laboratory Testing for Non-Criteria Antiphospholipid Antibodies: Antibodies Towards the Domain One of Beta2-Glycoprotein I (aDI)

Katrien M.J. Devreese

Part III Measuring or Monitoring Anticoagulants and Antithrombobotic Therapy

22. An Overview of Heparin Monitoring with the Anti-Xa Assay

Christina L. Dean

23. Ecarin Based Methods for Measuring Thrombin Inhibitors

Robert C. Gosselin

24. Assays to Monitor Bivalirudin

Simon Davidson

25. Monitoring of Anti-Platelet Therapy

Simon Davidson

PART IV Heparin Induced Thrombocytopenia (HIT) and Vaccine Induced Immune Thrombotic Thrombocytopenia (VITT)

26. Heparin Induced Thrombotic Thrombocytopenia (HITT) and Vaccine Induced Immune Thrombotic Thrombocytopenia (VITT): Similar by Different

Emmanuel J. Favaloro and Leonardo Pasalic

27. Functional Testing for Heparin Induced Thrombocytopenia using Whole Blood Multiplate Impedance Aggregometry

Marie-Christine Morel-Kopp

28. Multiple Electrode Aggregometry (Multiplate): Functional Assay for Vaccine Induced (Immune) Thrombotic Thrombocytopenia (VITT)

Yvonne Kong, Geoffrey Kershaw, Yuet Kee Suki Fu, and Freda Passam

29. Whole Blood Procoagulant Platelet Flow Cytometry Protocol for Heparin-Induced Thrombocytopenia (HIT) and Vaccine-Induced Immune Thrombotic Thrombocytopenia (VITT) Testing

Christine S. M. Lee, Matthew C. Powell, and Vivien M. Chen

30. Serotonin Release Assay: Functional Assay for Heparin and Vaccine Induced (Immune) Thrombotic Thrombocytopenia

Mayuko Kondo, Dea Donikian, and Timothy A. Brighton

PART V ADAMTS13

31. An Overview of Laboratory Testing for ADAMTS13

Emmanuel J. Favaloro and Leonardo Pasalic

32. Automated and Rapid ADAMTS13 Testing using Chemiluminescence: Utility for Identification or Exclusion of TTP and Beyond

Emmanuel J. Favaloro, Kent Chapman, Soma Mohammed, Ronny Vong, and Leonardo Pasalic

33. Identification of ADAMTS13 Inhibitors in Acquired TTP

Emmanuel J. Favaloro, Kent Chapman, Soma Mohammed, Ronny Vong, and Leonardo Pasalic

34. ADAMTS13 Activity: Screening Test Protocol

Gary W. Moore, Margaret Griffiths, and Nikolaus B. Binder

35. ADAMTS13 Activity Measurement by ELISA and Fluorescence Resonance Energy Transfer Assay

Gary W. Moore, Marcos Llusa, Margaret Griffiths, and Nikolaus B. Binder

36. ADAMTS13 Antibody and Inhibitor Assays

Gary W. Moore, Helga Vetr, and Nikolaus B. Binder

PART VI Bleeding Disorders

37. A Review of Factor VIII and Factor IX Assay Methods for Monitoring Extended Half-Life Products in Haemophilia A and B

Sunil Abraham an Elizabeth Duncan

38. Measuring Emicizumab Levels in the Hemostasis Laboratory

Geoffrey Kershaw and Caroline Dix

39. Chromogenic Factor VIII Assay for Patients with Haemophilia A and On Emicizumab Therapy

Olivia A. Yacoub and Elizabeth M. Duncan

40. Assessment of Platelet Function by Automated Light Transmission Aggregometry

Mayukh K. Sarkar and Christine Hinz

41. Assessment of Platelet Function by High-Throughput Screening Light Transmittance Aggregometry: Optimul Assay

Melissa V. Chan, Marie Lordkipanidzé, and Timothy D. Warner

42. Preparing Surrogate Abnormal Quality Samples for Platelet Function Studies and Viscoelastic Testing

Robert C. Gosselin

43. Laboratory Testing for von Willebrand Disease Using a Composite Rapid 3-Test Chemiluminescence-Based von Willebrand Factor Assay Panel

Emmanuel J. Favaloro, Soma Mohammed, Ronny Vong, and Leonardo Pasalic

44. Laboratory Testing for von Wilebrand Factor Activity by a Glycoproein Ib Binding Assay (VWF:GPIbR): HemosIL von Willebrand Factor Ristocetin Cofactor Activity on ACL TOP®

Omid Seidizadeh and Flora Peyvandi

45. Laboratory Testing for von Willebrand Factor:Factor VIII Binding for Diagnosis or Exclusion of Type 2N von Willebrand Disease: An Update

Emmanuel J. Favaloro, Soma Mohammed, Ronny Vong, and Leonardo Pasalic

46. Automation of a Factor XIII Activity Assay Utilizing a Plasma Blank Measurement

Kristi J. Smock and Karen A. Moser

PART VII Global Assays, Research Applications, and Postanalytical Considerations

47. Measurement of Blood Viscoelasticity using Thromboelastography

Oksana Volod and Alice Runge

48. The TEG 5000© System: System Description and Protocol for Measurements

Oksana Volod and Alice Runge

49. The TEG 6s© System: System Description and Protocol for Measurements

Oksana Volod and Alice Runge

50. The Quantra® System: System Description and Protocols for Measurements

Oksana Volod and Francesco Viola

51. Modified Rotational Thromboelastometry Protocol using Tissue Plasminogen Activator for Detection of Hypofibrinolysis and Hyperfibrinolysis

Julie Brogaard Laren, Christine Lodberg Hvas, and Anne-Mette Hvas

52. Protocol for the Investigation of Plasma and Whole Blood Clot Property of Fibrin Fiber Thickness using Scanning Electron Microscopy

Tengyi Cai, Conor McCafferty, Suelyn Van Den Helm, Natasha Letunica, Chantal Attard, Paul Monagle, and Vera Ignjatovic

53. Post-Analytical Issues in Hemostasis and Thrombosis Testing: An Update

Emmanuel J. Favaloro, Robert C. Gosselin, Leonardo Pasalic, and Giuseppe Lippi

Subject Index List…