Hematopoietic and Lymphoid Tissue in Culture

ISBN-13: 9781468416312 / Angielski / Miękka / 2012 / 182 str.

E. A. Luriya

Opis

This translation from Russian into English of the monograph Hematopoi- etic and Lymphoid Tissue in Culture by E. A. Luriya provides us for the first time with a comprehensive review of the massive amount of work carried out in the USSR in this important area of biological research. Historically, the pioneering tissue culture studies of Maksimov, Popov, Chasovnikov, and their colleagues working in Russia in the early part of this century provided much of the impetus for subsequent studies on lymphopoiesis and hematopoiesis in vitro and much of the basic information used subsequently to achieve immune responses in vitro and to develop the methodology for cloning hematopoietic stem cells. Particularly welcome in this translation is the addition of the Appendix, in which Drs. Luriya and Fridenshtein describe in some detail the methodology that has been developed in their laboratories for the study of a variety of cell types in cell and tissue culture. For many of us, this description provides information that had previously not been accessible to us: porosity of millipore fIlters in relation to growth properties; colony-forming cell adhesiveness; pro- liferation kinetics of stromal cells; differential effectiveness of various media used for culture of liver cells; details of washing procedures for fIlter substrates used in culture.

Spis treści

The Study of Hematopoietic and Lymphoid Tissues in Tissue Culture.- The Development of Tissue Culture Methods.- I. Hematopoietic Tissue In Vitro.- 1. Hematopoietic Tissue in Plasma Clot Culture.- 2. Monolayer Cultures of Hematopoietic Tissue.- 3. Suspension Cultures of Hematopoietic Tissue.- 4. Formation of Hematopoietic Foci in Agar Cultures.- 5. Organ Cultures of Hematopoietic Tissue.- Embryonic Liver.- Bone Marrow.- 6. A Comparative Assessment of the Various Methods of Hematopoietic Tissue Culture.- Duration of Hematopoiesis.- Myelopoiesis.- Erythropoiesis.- 7. Factors That Influence Differentiation of Hematopoietic Tissue In Vitro.- II. Possibilities of Differentiation of Cells Grown in Hematopoietic Tissue Cultures on Retransplantation In Vivo.- III. Lymphoid Tissue In Vitro.- 1. Lymphoid Tissue Explanted in a Plasma Clot.- Spleen.- Lymph Nodes.- Thymus.- Cells of the Peripheral Blood and Lymph.- 2. Monolayer Cultures of Lymphoid Tissue and Peripheral Blood Cells.- 3. Suspension Cultures of Lymphoid Tissue and Peripheral Blood Cells.- 4. Organ Cultures of Lymphoid Tissue.- Thymus.- Lymph Nodes.- 5. Comparison of Various Methods of Lymphoid Tissue Culture.- The Behavior of Lymphocytes.- Cell Transformations.- The Process of Regeneration.- Formation of Structures Characteristic of Interaction Between Lymphocytes and Stromal Cells.- IV. Immunological Functions of Lymphoid Tissue In Vitro.- 1. The Process of Antibody Formation.- Basic Mechanisms of Antibody Formation In Vivo and Aims of Research into This Process In Vitro.- Secondary Antibody Synthesis In Vitro.- Primary Antibody Synthesis In Vitro.- 2. The Delayed Hypersensitivity Reaction In Vitro.- Basic Mechanisms of the Delayed Hypersensitivity Reaction In Vivo and Aims of Research into This Process In Vitro.- Blast-Transformation, Recognition of Antigens, and the Cellular Immune Response In Vitro.- In Vitro Destruction of Target Cells by Lymphocytes from Preimmunized Donors.- Inhibition of Macrophage Migration.- The Simonsen Phenomenon.- Immunological Tolerance.- Conclusion.- V. Cell Lines in Lymphoid and Hematopoietic Tissue.- Conclusion.- Appendix. Recent Experience in Monolayer and Organ Cultures of Hematopoietic Tissue — E. A. Luriya and A. Ya. Fridenshtein.- 1. The Technique of Cloning Fibroblasts in Monolayer Cultures of Hematopoietic and Lymphoid Cells and Its Use to Study Stromal Precursor Cells.- 2. The Method of Multiple Organ Cultures of Hematopoietic and Lymphoid Tissue.- References.