Hematopoietic Stem Cells

ISBN-13: 9781071626818 / Angielski / Miękka / 2023

Opis

This detailed volume collects updates on the technical advances in hematopoietic stem cell research and incorporates new techniques focused on the molecular/genetic, cellular, and whole organism levels. Exploring methods that apply stress to hematopoiesis, the book also contains chapters focused on better understanding the role of hematopoietic niches and their cellular components, as well as in vivo models that test and quantitate stem cell function and are key to further development of therapeutic applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Hematopoietic Stem Cells: Methods and Protocols serves as a valued addition to laboratories focused on understanding hematopoietic stem cell biology and the therapeutic advances that can be derived from it.

Spis treści

Part I: Overview

 

1. The Factory of Blood Production: Hematopoietic Stem Cells

            Jonathan Hoggatt and Louis M. Pelus

 

Part II: Molecular Modification and Evaluation

 

2. Detection of DNA Damage in Hematopoietic Stem Cells

            Saipriya Ayyar and Isabel Beerman

 

3. Evaluating Histone Acetylation in Mouse Hematopoietic Stem and Progenitor Cells Using Chromatin Immunoprecipitation

            Liqiong Liu

 

4. CRISPR Gene Editing of Hematopoietic Stem and Progenitor Cells

            Reza Shahbazi, Patricia Lipson, Karthikeya S.V. Gottimukkala, Daniel D. Lane, and Jennifer E. Adair

 

5. Lentiviral Transduction of Nonhuman Primate Hematopoietic Stem and Progenitor Cells

            Chuanfeng Wu, So Gun Hong, Aylin Bonifacino, and Cynthia E. Dunbar

 

Part III: Cellular Isolation and Characterization

 

6. Identification of Nonhuman Primate Hematopoietic Stem and Progenitor Cells

            Stefan Radtke and Hans-Peter Kiem

 

7. Isolation and Characterization of Fetal Liver Hematopoietic Stem Cells

            Diego A. López and Anna E. Beaudin

 

8. Utilizing CyTOF to Examine Hematopoietic Stem and Progenitor Phenotype

            Safa F. Mohamad and Maegan L. Capitano

 

9. Hematopoietic Stem Cell Identification Post-Radiation

            Andrea M. Patterson, Christie M. Orschell, and Louis M. Pelus

 

Part IV: The Hematopoietic Niche and Accessory Cells

 

10. Intravital Microscopy for Hematopoietic Studies

            Myriam Haltalli and Cristina Lo Celso

 

11. Laser Micromachining of Bone as a Tool for Studying Bone Marrow Biology

            Christa Haase, Dmitry Richter, and Charles P. Lin

 

12. MSC and HSPC Co-Culture: Mimicking Ex Vivo Bone Marrow Niche

            Pratibha Singh

 

13. Isolation of Thymus Stromal Cells from Human and Murine Tissue

            Karin Gustafsson and David T. Scadden

 

Part V: Transplantation and In Vivo Models

 

14. Experimental Models of Mouse and Human Hematopoietic Stem Cell Transplantation

            Scott H. Cooper, Maegan L. Capitano, and Hal E. Broxmeyer

 

15. Hematopoietic Stem and Progenitor Cell Identification and Transplantation in Zebrafish

            Ellen Fraint, Peng Lv, Feng Liu, Teresa V. Bowman, and Owen J. Tamplin

 

16. Establishing a Murine Model of the Hematopoietic Acute Radiation Syndrome

            P. Artur Plett, Louis M. Pelus, and Christie M. Orschell

 

17. Purinergic Signaling and Its Role in Mobilization of Bone Marrow Stem Cells

            Malwina Suszynska, Mateusz Adamiak, Arjun Thapa, Monika Cymer, Janina Ratajczak, Magdalena Kucia, and Mariusz Z. Ratajczak