Hematological Malignancies

ISBN-13: 9781627033565 / Angielski / Twarda / 2013 / 304 str.

Magdalena Czader

Opis

Increased knowledge on the pathogenesis of hematologic diseases has been translated into diagnostic and prognostic applications. Hematopathology and laboratory hematology were among the first disciplines to embrace molecular diagnostics. Hematological Malignancies: Methods and Protocols, explores molecular-based assays frequently used in the routine diagnostic hematopathology and laboratory hematology. Many of these protocols were initially developed as research applications and were further refined as they transitioned to the diagnostic laboratory. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Hematological Malignancies: Methods and Protocols aids scientist in the continuing study of tests essential for contemporary laboratory diagnostics of hematological neoplasms.

Spis treści

1. Quantitative BCR-ABL1 RQ-PCR Fusion Transcript Monitoring in Chronic Myelogenous Leukemia

Franklin R. Moore, Carole B. Rempfer and Richard D. Press

 

2. Detection of BCR-ABL1 Kinase Domain Mutations Causing Imatinib Resistance in Chronic Myelogenous Leukemia

Franklin R. Moore, Fei Yang, and Richard D. Press

 

3. Laboratory Detection of JAK2^V617F in Human Myeloproliferative Neoplasms

Jonathan S. Kui, Rosanny Espinal–Witter, and Y. Lynn Wang

 

4. c-kit Mutational Analysis in Paraffin Material

Karl Sotlar

 

5. Detection of Recurrent Cytogenetic Abnormalities in Acute Lymphoblastic and Myeloid Leukemias using Fluorescence In Situ Hybridization

Gail H. Vance

 

6. Liquid Bead Array Technology in the Detection of Common Translocations in Acute andChronic Leukemias

 

7. Molecular Genetic Tests for FLT3, NPM1, and CEBPA in Acute Myeloid Leukemia

Qing Zhang and Gail H. Vance

 

8. Flow Cytometric Monitoring of Residual Disease in Acute Leukemia

Brent L. Wood

 

9. Chimerism Analysis Following Hematopoietic Stem Cell Transplantation

Kathleen M. Murphy

 

10. Detection of Clonal Immunoglobulin Heavy Chain Gene Rearrangements by the Polymerase Chain Reaction and Capillary Gel Electrophoresis

Hongxin Fan and Ryan S. Robetorye

 

11. Detection of Clonal T-cell Receptor Beta and Gamma Chain Gene Rearrangement by Polymerase Chain Reaction and Capillary Gel Electrophoresis

Hongxin Fan and Ryan S. Robetorye

 

12. Detection of Genetic Translocations in Lymphoma using Fluorescence in situ Hybridization

Kaaren K. Reichard and Sheldon Robinett

 

13. Molecular Detection of t(14;18)(q32;q21) in Follicular Lymphoma

C. Cameron Yin and Rajyalakshmi Luthra

 

14. Detection of t(11;14)(q13;q32) in Mantle Cell Lymphoma by Polymerase Chain Reaction

C. Cameron Yin and Rajyalakshmi Luthra

 

15. Detection of t(2;5)(p23;q35) in Anaplastic Large Cell Lymphoma by Long-range Nested Polymerase Chain Reaction Assay

C. Cameron Yin, and Rajyalakshmi Luthra

 

16. EBER In Situ Hybridization for Epstein-Barr Virus

Lawrence M. Weiss, Yuan-Yuan Chen

 

17. Epstein-Barr virus (EBV) Load Determination Using Real-time Quantitative Polymerase Chain Reaction

Hongxin Fan and Ryan S. Robetorye

 

18. Molecular and Immunohistochemical Detection of Kaposi Sarcoma Herpesvirus / Human Herpesvirus-8

Amy Chadburn, Janet Wilson, Y. Lynn Wang

 

19. Detection of Cytomegalovirus Infection by Quantitative Polymerase Chain Reaction

Magdalena Czader, Kristin Post, Liang Cheng

 

20. Thiopurine S-Methyltransferase Pharmacogenetics in Childhood Acute Lymphoblastic Leukemia

Jun J. Yang and Deepa Bhojwani

 

21. MicroRNA Expression Profiling Using Microarrays

Cassandra Love and Sandeep Dave

Blurb

Increased knowledge on the pathogenesis of hematologic diseases has been translated into diagnostic and prognostic applications.   Hematopathology and laboratory hematology were among the first disciplines to embrace molecular diagnostics.  Hematological Malignancies:Methods and Protocols, explores molecular-based assays frequently used in the routine diagnostic hematopathology and laboratory hematology.  Many of these protocols were initially developed as research applications and were further refined as they transitioned to the diagnostic laboratory. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 Authoritative and practical, Hematological Malignancies: Methods and Protocols aids scientist in the continuing study of tests essential for contemporary laboratory diagnostics of hematological neoplasms.