ISBN-13: 9781493959075 / Angielski / Miękka / 2016 / 258 str.
ISBN-13: 9781493959075 / Angielski / Miękka / 2016 / 258 str.
This Methods in Molecular Biology (TM) book details methods and and protocols used to study Helicobacter infection. Includes lists of materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and known pitfalls.
1. Introduction
JeanMarie Houghton
2. Helicobacter pylori: An Overview
Jennifer M. Noto and Richard M. Peek, Jr.
3. Perspectives on Methodology for in vitro Culture of Helicobacter pylori
Timothy L. Cover
4. Successful Culture Techniques for Helicobacter Species : General Culture Techniques for Helicobacter pylori
Jeannette M. Whitmire and D. Scott Merrell
5. Successful Culture Techniques for Helicobacter Species: Establishing H. pylori Cultures From Infected Rodents
Jeannette M. Whitmire and D. Scott Merrell
6. Successful Culture Techniques for Helicobacter Species: Verification of Helicobacter Identity Using 16S rRNA Gene Sequence Analysis
Jeannette M. Whitmire and D. Scott Merrell
7. The Helicobacter pylori cag Pathogenicity Island
Jennifer M. Noto and Richard M. Peek, Jr.
8. Genetic Manipulation of a Naturally Competent Bacterium, Helicobacter pylori
Jennifer M. Noto and Richard M. Peek, Jr.
9. A Method for Short-term Culture of Human Gastric Epithelial Cells to Study the Effects of Helicobacter pylori
Marina Leite and Ceu Figueiredo 10. Cell Culture Based Assays to Test for Bacterial Adherence and Internalization Deepa Raju, David Rizzuti and Nicola L. Jones 11. Cell Culture Assays to Evaluate Bacterial Toxicity and Virulence Deepa Raju, David Rizzuti, Nicola L. Jones 12. Rodent Models of Helicobacter Infection, Inflammation and Disease Songhua Zhang and Steven F. Moss 13. Bacterial Culture and Inoculation of Mice (Simple Infection) Brian M. Gray and Kathryn A. Eaton 14. Adoptive Transfer of Splenocytes to Immunocompromised Mice Brian M. Gray and Kathryn A. Eaton 15. Isolation of Gastric Lamina Propria Leukocytes (LPL) Brian M. Gray and Kathryn A. Eaton 16. Delayed-type Hypersensitivity Determination Brian M. Gray and Kathryn A. Eaton 17. Necropsy, Blood, Tissue Collection & mRNA Isolation for Detection of Host Cytokine Gene Expression Brian M. Gray and Kathryn A. Eaton 18. Animal Models of Helicobacter Induced Disease – Methods to Successfully Infect the Mouse Nancy S. Taylor and James G. Fox 19. Verifying and Quantifying Helicobacter pylori Infection Status of Research Mice Mark T Whary, Zhongming Ge and James G Fox 20. Mouse Models of Helicobacter-induced Gastric Cancer: Use of Cocarcinognes Richard L. Ferrero, John E. Wilson, and Phil Sutton 21. Gastric Helicobacter spp. in Animal Models: Pathogenesis and Modulation by Extragastric Coinfections Arlin B. Rogers 22. Histologic Scoring of Gastritis and Gastric Cancer in Mouse Models Arlin B. Rogers 23. Innate Immune Responses to Helicobacter pylori Infection- An Overview Milan K. Patel, Melanie I. Trombly and Evelyn A. Kurt-Jones 24. Methods for In vivo and In vitro Analysis of Innate Immune Responses to Helicobacter pylori Infection Milan K. Patel, Glennice N. Bowen, Anna M. Cerny and Evelyn A. Kurt-Jones 25. Techniques for Following Labeled Cells In vivo: Use of X/Y FISH, Techniques to Optimize Fluorescent Detection, and Beta-galactosidase Detection 26. In vivo Measurement of Helicobacter pylori Infection Marjan Mohammadi, Samaneh Saberi Kashani, Yeganeh Talebkhan Garoosi, Sahar Jahangiri Tazehkand
Deepa Raju, David Rizzuti and Nicola L. Jones
11. Cell Culture Assays to Evaluate Bacterial Toxicity and Virulence
Deepa Raju, David Rizzuti, Nicola L. Jones
12. Rodent Models of Helicobacter Infection, Inflammation and Disease
Songhua Zhang and Steven F. Moss
13. Bacterial Culture and Inoculation of Mice (Simple Infection)
Brian M. Gray and Kathryn A. Eaton
14. Adoptive Transfer of Splenocytes to Immunocompromised Mice
Brian M. Gray and Kathryn A. Eaton
15. Isolation of Gastric Lamina Propria Leukocytes (LPL)
Brian M. Gray and Kathryn A. Eaton
16. Delayed-type Hypersensitivity Determination
Brian M. Gray and Kathryn A. Eaton
17. Necropsy, Blood, Tissue Collection & mRNA Isolation for Detection of Host Cytokine Gene Expression
Brian M. Gray and Kathryn A. Eaton
18. Animal Models of Helicobacter Induced Disease – Methods to Successfully Infect the Mouse
Nancy S. Taylor and James G. Fox
19. Verifying and Quantifying Helicobacter pylori Infection Status of Research Mice
Mark T Whary, Zhongming Ge and James G Fox
20. Mouse Models of Helicobacter-induced Gastric Cancer: Use of Cocarcinognes
Richard L. Ferrero, John E. Wilson, and Phil Sutton
21. Gastric Helicobacter spp. in Animal Models: Pathogenesis and Modulation by Extragastric Coinfections
Arlin B. Rogers
22. Histologic Scoring of Gastritis and Gastric Cancer in Mouse Models
Arlin B. Rogers
23. Innate Immune Responses to Helicobacter pylori Infection- An Overview
Milan K. Patel, Melanie I. Trombly and Evelyn A. Kurt-Jones
24. Methods for In vivo and In vitro Analysis of Innate Immune Responses to Helicobacter pylori Infection
Milan K. Patel, Glennice N. Bowen, Anna M. Cerny and Evelyn A. Kurt-Jones
25. Techniques for Following Labeled Cells In vivo: Use of X/Y FISH, Techniques to Optimize Fluorescent Detection, and Beta-galactosidase Detection
Michael Craig, Michael Schumacher and Yana Zavros
26. In vivo Measurement of Helicobacter pylori Infection
Marjan Mohammadi, Samaneh Saberi Kashani, Yeganeh Talebkhan Garoosi, Sahar Jahangiri Tazehkand
3. Perspectives on Methodology for in vitro Culture of Helicobacter pylori
Timothy L. Cover
4. Successful Culture Techniques for Helicobacter Species : General Culture Techniques for Helicobacter pylori
Jeannette M. Whitmire and D. Scott Merrell
5. Successful Culture Techniques for Helicobacter Species: Establishing H. pylori Cultures From Infected Rodents
Jeannette M. Whitmire and D. Scott Merrell
6. Successful Culture Techniques for Helicobacter Species: Verification of Helicobacter Identity Using 16S rRNA Gene Sequence Analysis
Jeannette M. Whitmire and D. Scott Merrell
7. The Helicobacter pylori cag Pathogenicity Island
Jennifer M. Noto and Richard M. Peek, Jr.
8. Genetic Manipulation of a Naturally Competent Bacterium, Helicobacter pylori
Jennifer M. Noto and Richard M. Peek, Jr.
9. A Method for Short-term Culture of Human Gastric Epithelial Cells to Study the Effects of Helicobacter pylori
Marina Leite and Ceu Figueiredo 10. Cell Culture Based Assays to Test for Bacterial Adherence and Internalization Deepa Raju, David Rizzuti and Nicola L. Jones 11. Cell Culture Assays to Evaluate Bacterial Toxicity and Virulence Deepa Raju, David Rizzuti, Nicola L. Jones 12. Rodent Models of Helicobacter Infection, Inflammation and Disease Songhua Zhang and Steven F. Moss 13. Bacterial Culture and Inoculation of Mice (Simple Infection) Brian M. Gray and Kathryn A. Eaton 14. Adoptive Transfer of Splenocytes to Immunocompromised Mice Brian M. Gray and Kathryn A. Eaton 15. Isolation of Gastric Lamina Propria Leukocytes (LPL) Brian M. Gray and Kathryn A. Eaton 16. Delayed-type Hypersensitivity Determination Brian M. Gray and Kathryn A. Eaton 17. Necropsy, Blood, Tissue Collection & mRNA Isolation for Detection of Host Cytokine Gene Expression Brian M. Gray and Kathryn A. Eaton 18. Animal Models of Helicobacter Induced Disease – Methods to Successfully Infect the Mouse Nancy S. Taylor and James G. Fox 19. Verifying and Quantifying Helicobacter pylori Infection Status of Research Mice Mark T Whary, Zhongming Ge and James G Fox 20. Mouse Models of Helicobacter-induced Gastric Cancer: Use of Cocarcinognes Richard L. Ferrero, John E. Wilson, and Phil Sutton 21. Gastric Helicobacter spp. in Animal Models: Pathogenesis and Modulation by Extragastric Coinfections Arlin B. Rogers 22. Histologic Scoring of Gastritis and Gastric Cancer in Mouse Models Arlin B. Rogers 23. Innate Immune Responses to Helicobacter pylori Infection- An Overview Milan K. Patel, Melanie I. Trombly and Evelyn A. Kurt-Jones 24. Methods for In vivo and In vitro Analysis of Innate Immune Responses to Helicobacter pylori Infection Milan K. Patel, Glennice N. Bowen, Anna M. Cerny and Evelyn A. Kurt-Jones 25. Techniques for Following Labeled Cells In vivo: Use of X/Y FISH, Techniques to Optimize Fluorescent Detection, and Beta-galactosidase Detection 26. In vivo Measurement of Helicobacter pylori Infection Marjan Mohammadi, Samaneh Saberi Kashani, Yeganeh Talebkhan Garoosi, Sahar Jahangiri Tazehkand
Deepa Raju, David Rizzuti and Nicola L. Jones
11. Cell Culture Assays to Evaluate Bacterial Toxicity and Virulence
Deepa Raju, David Rizzuti, Nicola L. Jones
12. Rodent Models of Helicobacter Infection, Inflammation and Disease
Songhua Zhang and Steven F. Moss
13. Bacterial Culture and Inoculation of Mice (Simple Infection)
Brian M. Gray and Kathryn A. Eaton
14. Adoptive Transfer of Splenocytes to Immunocompromised Mice
Brian M. Gray and Kathryn A. Eaton
15. Isolation of Gastric Lamina Propria Leukocytes (LPL)
Brian M. Gray and Kathryn A. Eaton
16. Delayed-type Hypersensitivity Determination
Brian M. Gray and Kathryn A. Eaton
17. Necropsy, Blood, Tissue Collection & mRNA Isolation for Detection of Host Cytokine Gene Expression
Brian M. Gray and Kathryn A. Eaton
18. Animal Models of Helicobacter Induced Disease – Methods to Successfully Infect the Mouse
Nancy S. Taylor and James G. Fox
19. Verifying and Quantifying Helicobacter pylori Infection Status of Research Mice
Mark T Whary, Zhongming Ge and James G Fox
20. Mouse Models of Helicobacter-induced Gastric Cancer: Use of Cocarcinognes
Richard L. Ferrero, John E. Wilson, and Phil Sutton
21. Gastric Helicobacter spp. in Animal Models: Pathogenesis and Modulation by Extragastric Coinfections
Arlin B. Rogers
22. Histologic Scoring of Gastritis and Gastric Cancer in Mouse Models
Arlin B. Rogers
23. Innate Immune Responses to Helicobacter pylori Infection- An Overview
Milan K. Patel, Melanie I. Trombly and Evelyn A. Kurt-Jones
24. Methods for In vivo and In vitro Analysis of Innate Immune Responses to Helicobacter pylori Infection
Milan K. Patel, Glennice N. Bowen, Anna M. Cerny and Evelyn A. Kurt-Jones
25. Techniques for Following Labeled Cells In vivo: Use of X/Y FISH, Techniques to Optimize Fluorescent Detection, and Beta-galactosidase DetectionMichael Craig, Michael Schumacher and Yana Zavros
26. In vivo Measurement of Helicobacter pylori Infection
Marjan Mohammadi, Samaneh Saberi Kashani, Yeganeh Talebkhan Garoosi, Sahar Jahangiri Tazehkand
Identifying Helicobacter infection as the leading cause of peptic ulcer disease and gastric cancer has dramatically altered the treatment of these disease states. Over the last several decades, scientists have come to understand that the interplay between the bacteria, the host, and the environment all contribute to the clinical outcome of infection. In Helicobacter Species: Methods and Protocols, expert researchers in the field detail many of the methods and which are now commonly used to study Helicobacter infection. These include protocols and methods that have evolved over time, and standards across the field have been established which are essential for optimal outcomes and to allow comparison of data across different laboratories. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Helicobacter Species: Methods and Protocols seeks to aid scientists in further study of this crucially important research into Helicobacter research.
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