Helicobacter Pylori

ISBN-13: 9781071613016 / Angielski / Twarda / 2021 / 234 str.

Sinead M. Smith

Recenzje

“The purpose is to provide clinicians and translational and basic scientists with an understanding of the clinical aspects of H. pylori infection and diagnostic testing, as well as a toolkit for performing the key laboratory protocols required to further our knowledge of the host. … This is an excellent book that provides up-to-date information about the methods and protocols necessary to understand more about the study of H. pylori. … It will be highly useful for the intended audience.” (Omer Iqbal, Doody's Book Reviews, July 30, 2021)

Spis treści

1. An Overview of Helicobacter pylori Infection

Rebecca FitzGerald and Sinéad Smith

 

2. The Urea Breath Test for the Non-Invasive Detection of Helicobacter pylori

Anthony O’Connor

 

3. Real-time Diagnosis of Helicobacter pylori during Endoscopy by Gastric Juice Analysis

Cristiano Spada and Stefania Piccirelli

 

4. Molecular Detection of Antibiotic-resistant Helicobacter pylori

Denise Brennan, Colm O’Morain, Deirdre McNamara, and Sinéad Smith

 

5. Endoscopic Detection of Helicobacter pylori by the Rapid Urease Test

Vikrant Parihar and Deirdre McNamara

 

6. Culture-based Antimicrobial Susceptibility Testing for Helicobacter pylori

Philippe Lehours and Francis Mégraud

 

7. Purification of Total RNA from Stomach Tissue Biopsies

Rebecca FitzGerald, Malini Devapal, John J. Hickey, Dermot Kelleher, and Sinéad Smith

 

8. Gene Expression Analysis by Reverse Transcription Quantitative PCR

Eva M. Campion and Sinéad T. Loughran

 

9. A Method to Investigate the Helicobacter pylori-associated DNA Methylome

Hae Dong Woo and Zdenko Herceg

 

10. Profiling the Gastrointestinal Microbiota

Brunella Posteraro, Flavio De Maio, and Antonio Gasbarrini

 

11. Helicobacter pylori Virulence Factor Genotyping

Rebecca FitzGerald, Chavi Sinha, Abbas Yadegar, and Sinéad Smith

 

12. Generation of Isogenic Mutant Strains of Helicobacter pylori

Stephanie L. Servetas, Jeannette M. Whitmire, and D. Scott Merrell

 

13. Isolation of Outer Membrane Vesicles from Helicobacter pylori

 Henry J. Windle

 

14. A Mouse Model of Helicobacter pylori Infection

Tanmoy Kumar Dey, Bipul Chandra Karmakar, Avijit Sarkar, Sangita Paul, and Asish Kumar Mukhopadhyay

 

15. Investigating the Interaction of Helicobacter pylori with the Gastric Mucosa

Marguerite Clyne, Ciara Dunne, and Brendan Dolan

 

16. Evaluating Host Responses to Helicobacter pylori using ELISA and Western Blot

Ciara Nulty, Gillian Barber, Ewelina Flis, and Emma M. Creagh

 

17. Analysis of Innate Immune Responses to Helicobacter pylori

Michelle Chonwerawong and Richard L. Ferrero

 

18. Analysis of T Cell Responses to Helicobacter pylori Infection

Kazuyo Kaneko and Karen Robinson

 

19. Miniaturised In Vitro Assays to Study Cellular Phenotypic Characteristics: Proliferation, Migration, Invasion, and Anoikis-resistance

Niamh McNamee and Lorraine O’Driscoll

Blurb

This book brings together a collection of methods for the successful detection, culture and investigation of H. pylori, and its associated diseases.  Chapters guide the reader through overviews on H. pylori infection, protocols for analyzing human clinical samples, and protocols for characterizing H. pylori, its functional components and its interactions with host cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Helicobacter pylori: Methods and Protocols aims to be a useful practical guide to researchers to help further their study in this field.