Hedgehog Signaling Protocols

ISBN-13: 9781617377211 / Angielski / Miękka / 2010 / 256 str.

Jamila I. Horabin

Opis

Intended for Molecular Biologists, Biochemists, and Geneticists interested in manipulating and analyzing the Hedgehog Signaling Pathway, this volume offers various detailed chapters on experimental description, including anecdotal pointers and notes.

Spis treści

TABLE OF CONTENTS Chapter 1. Purifying the Hedgehog protein and its variants by Darren P. Baker, Frederick R. Taylor and R. Blake Pepinsky Chapter 2. Application of Sonic hedgehog to the developing chick limb by Eva Tiecke and Cheryll Tickle Chapter 3. Manipulation of Hedgehog signaling in Xenopus by means of embryo microinjection and application of chemical inhibitors by Thomas Hollemann, Emmanuel Tadjuidje, Katja Koebernick & Tomas Pieler Chapter 4. Isolation of rat telencephalic neural explants to assay Shh GABAergic interneuron differentiation-inducing activity by Rina Mady and Jhumku D. Kohtz Chapter 5. Genetic Analysis of the Vertebrate Hedgehog Signaling Pathway Using Muscle Cell Fate Specification in the Zebrafish Embryo by Sudipto Roy Chapter 6. Efficient Manipulation of Hedgehog/GLI Signaling using Retroviral Expression Systems by Maria Kasper, Gerhard Regl, Thomas Eichberger, Anna-Maria Frischauf and Fritz Aberger Chapter 7. Cell Surface Marker and Cell Cycle Analysis, Hedgehog Signaling, and Flow Cytometry by Kristina Detmer and Ronald E. Garner Chapter 8. Detecting tagged Hedgehog with Intracellular and Extracellular Immunocytochemistry for functional analysis by Ainhoa Callejo, Luis Quijada and Isabel Guerrero Chapter 9. Confocal Analysis of Hedgehog Morphogenetic Gradient Coupled with Fluorescent in situ Hybridization of Hedgehog Target Genes by Armel Gallet and Pascal P. Thérond Chapter 10. RNAi in the Hedgehog signaling pathway: pFRiPE, a vector for temporally and spatially controlled RNAi in Drosophila by Eric Marois and Suzanne Eaton Chapter 11. Germline clone analysis for maternally acting Drosophila Hedgehog components by Erica M. Selva and Beth E. Stronach Chapter 12. Clonal analysis of Hedgehog signaling in Drosophila somatic tissues by Christine M. Bankers and Joan E. Hooper Chapter 13. GAL4/UAS targeted gene expression for studyingDrosophila Hedgehog signaling by Denise Busson and Anne-Marie Pret Chapter 14. Biochemical Fractionation of Drosophila Cells by Melanie Stegman and David Robbins Chapter 15. Using Immunoprecipitation to Study Protein-protein Interactions in the Hedgehog Signaling Pathway by Chao Tong and Jin Jiang Chapter 16. Sequence analyses to study the evolutionary history and cis-regulatory elements of Hedgehog genes by Ferenc Müller and Anne-Gaelle Borycki

Blurb

In both vertebrates and invertebrates, Hedgehog signaling regulates the development of the tissues and structures of the body. In addition, many cancers and diseases arise as a result of misregulation of the pathway, which makes it an incredibly important system to understand. This volume examines various methods used in the analysis of the Hedgehog signaling pathway, bringing together different lines of analyses into one accessible and comprehensive text. This volume provides a single source for investigators, presenting several different experimental approaches, which are all varied and broad in nature, as well as procedures for a variety of different model systems. Researchers will find this volume to be an invaluble tool as they work towards deeper understanding of the underlying molecular events that the Hedgehog signaling pathway regulates.