Heat Stress Signal Transduction in Plant Cells

ISBN-13: 9783639004205 / Angielski / Miękka / 2008 / 112 str.

Ahmed Mansour

Opis

Plant tolerance to heat stress proved to be entirely dependent on the signa­ling flow of information by which the plant can sense the changes in its surrounding environment and signal its genes to respond by producing special proteins to protect it-self. Any changes or manipulations in this signaling flow of information will presumably lead to a modification in the genetic expression inside the plant cells, consequently, changing plant per­formance. Understanding these signaling events in response to heat may help us to produce heat tolerant plants capable to stand high temperature stress. In the present investigation, the results showed that a heat activated MAP kinase cascade, involving heat activated MAP kinase (HAMK), played an essential role in heat shock gene expression in tobacco BY-2 cells. In order to determine if heat activation of HAMK involved additional pathways of signaling we studied the upstream regulation of HAMK, including membrane fluidization and reorganization of cytoskeleton. The activation of HAMK and accumulation of heat responsive HSFs, HSP70 and HSP27 proteins were used as end-point markers in these experiments. It is concluded that the heat shock response, as measured by HAMK activation and heat shock proteins accu­mulation required PKC activation, membrane fluidization and reorganization of the cytoskeleton. A comparative bioinformatic explanation of similarities between tobacco heat shock genes and their counterparts in different organisms revealed a high degree of evolutionary conservation in the corres­ponding domains, indicating similar function in different species.

O autorze

Mansour, Ahmed Dr. Ahmed Mansour is an Egyptian scientist who obtained his Ph.D. degree in (Plant Signal transduction) in Faculty of agriculture, Zagazig University, Egypt, in collaboration with McGill University, Canada, where he spends two years of full experimental work, and his dissertation research entitled (Manipulation of Genetic information in studying plant performance). He have been invited and participated in many international scientific meetings and conferences in molecular genetics around the world. He also has many scientific publications in the field of Molecular Genetics and Bioninformatics.