Handbook of Elispot: Methods and Protocols

ISBN-13: 9781493985661 / Angielski / Twarda / 2018 / 251 str.

Alexander E. Kalyuzhny

Spis treści

Preface…

Table of Contents…

Contributing Authors…

1. Challenges in Developing Protein Secretion Assays at a Single-Cell Level

Yoshitaka Shirasaki and Osamu Ohara

2. To Count of Not to Count: ELISPOT Evaluation Revisited

Sylvia Janetzki

3. Essential Controls for ELISpot Assay

Jodi Hagen and Alex Kalyuzhny

4. Automatic Search of Spots and Color Classification in ELISPOT Assay

Sergey S. Zadorozhny and Nikolai N. Martynov

5. Four Color ImmunoSpot ® Assays for Identification of Effector T Cell Lineages

Jodi Hanson, Diana R. Roen, and Paul V. Lehmann

6. Detection of Cross-Reactive B Cells Using the FluoroSpot Assay

Peter Jahnmatz and Niklas Ahlborg

7. Multiplex ImmunoSpot® Assays for the Study of Functional B Cell Subpopulations

Diana R. Roen, Jodi Hanson, and Paul V. Lehmann

8. Detecting All Immunoglobulin Classes and Subclasses in Multiplex 7 Color ImmunoSpot® Assays

Richard Caspell and Paul V. Lehmann

9. Multiplexing T- and B-Cell FLUOROSPOT Assays: Experimental Validation of the Multi-Color ImmunoSpot® Software Based on Center of Mass Distance Algorithm

Alexey Y. Karulin, Zoltán Megyesi, Richard Caspell, Jodi Hanson, and Paul V. Lehmann

10. Multi-Color FLUOROSPOT Counting Using ImmunoSpot® Fluoro-X™ Suite

Zoltán Megyesi, Paul V. Lehmann, and Alexey Y. Karulin

11. B-Cell ELISpot Assay to Quantify Antigen-Specific Antibody-Secreting Cells in Human Peripheral Blood Mononuclear Cells

Haw Hwai, Yi-Ying Chen, and Siang-Jong Tzeng

12. Identification of Novel Mycobacterial Targets for Murine CD4+T Cells by IFN ELISPOT

Alison J. Johnson, Steven C. Kennedy, Tony W. Ng, and Steven A. Porcelli

13. ELISPOT-Based “Multi-Color FluoroSpot” to Study Type-Specific and Cross-Reactive Responses in Memory B Cells after Dengue and Zika Virus Infections

Paulina Andrade, Josefina Coloma, and Eva Harris

14. Cultured ELISpot Assay to Investigate Dengue Virus Specific T Cell Responses

Chandima Jeewandara, Graham S. Ogg, and Gathsaurie Neelika Malavige

15. Ex Vivo ELISpot Assay to Investigate Dengue Virus Specific T Cell Responses

Gathsaurie Neelika Malavige

16. Ex Vivo ELISpot Assay to Investigate iNKT Cell Responses in Acute Dengue Infection

Achala Indika Kamalasasa and Gathsaurie Neelika Malavige

17. Dendritic Cell-Based ELISpot Assay for Assessing T Cell-IFN- γ Responses in Human Peripheral Blood Mononuclear Cells to Dengue Envelope Proteins

Peifang Sun and Monika Simmons

18. Utilization of Feline ELISpot to Evaluate the Immunogenicity of a T Cell-Based FIV MAP Vaccines

Bikash Sahay, Alek M. Aranyos, Andrew McAvoy, and Janet K. Yamamoto

19. Detection and Quantification of Influenza A/H1N1 Virus-Specific Memory B Cells in Human PBMCs using ELISpot Assay

Iana H. Haralambieva, Inna G. Ovsyannikova, Richard B. Kennedy, and Gregory A. Poland

Blurb

This third edition volume expands on the previous editions with new protocols and up-to-date techniques to help researchers excel in this field. The chapters in this book cover topics such as ELISpot’s sibling (FluoroSpot); ELISpot for influenza, Dengue virus, and tuberculosis; and vaccine research, essentials control, and assay evaluation in ELISpot. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Handbook of ELISPOT: Methods and Protocols, Third Edition is a valuable resource for both novice researchers and experienced ELISpot users.