Handbook for Azospirillum: Technical Issues and Protocols

ISBN-13: 9783319065410 / Angielski / Twarda / 2015 / 514 str.

Fabricio Dario Cassan; Okon Yaacov; Cecilia Creus

Opis

The functional analysis of plant-microbe interactions has re-emerged in the past 10 years due to spectacular advances in integrative study models. This book summarizes basic and technical information related to the plant growth promoting rhizobacteria (PGPR) belonging to the genus Azospirillum, considered to be one of the most representative PGPR last 40 years. We include exhaustive information about the general microbiology of genus Azospirillum, their identification strategies; the evaluation of plant growth promoting mechanisms, inoculants technology and agronomic use of these bacteria and some special references to the genetic technology and use.

Spis treści

Part I  Biochemistry and Molecular Biology Toolbox.- Chapter 1 Isolation, Identification and Biochemical Characterization of Azospirillum spp. and other Nitrogen-Fixing Bacteria.- Chapter 2 Molecular Tools for Identification and Characterization of Plant Growth Promoting Rhizobacteria with emphasis in Azospirillum spp.- Chapter 3 In situ Localization and Strain-Specific Quantification of Azospirillum and other Plant Growth Promoting Rhizobacteria Using Antibodies and Molecular Probes.- Chapter 4 Molecular Tools to Study Azospirillum sp. and other Related Plant Growth Promoting Rhizobacteria.- Chapter 5 Genomic Tools for the Study of Azospirillum and other Plant Growth Promoting Rhizobacteria.- Part II Physiology and Metabolism Toolbox.- Chapter 6 Chemotaxis and other Forms of Taxis Behaviors in Azospirillum spp.- Chapter 7 Phytohormones and other Plant Growth Regulators Produced by PGPR: The Genus Azospirillum.- Chapter 8 Inorganic Nitrogen Metabolism in Azospirillum spp.- Chapter 9 Nitric Oxide in Azospirillum and Related Bacteria: Production and Effects.- Chapter 10 Cell Aggregation, Attachment and Plant Interaction.- Chapter 11 Methods for Studying Biofilms in Azospirillum and other Plant Growth Promoting Rhizobacteria.- Chapter 12 Methods for Studying Phenotypic Variation in Azospirillum.- Chapter 13 Polyhydroxybutyrate in Azospirillum brasilense.- Chapter 14 Siderophores Production by Azospirillum: Biological Importance, Assessing methods and Biocontrol activity.- Chapter 15 Cell-Cell Communication in Azospirillum and Related Plant Growth Promoting Rhizobacteria.- Chapter 16 Methods to Study 1-Aminocyclopropane-1-Carboxylate Deaminase in Plant Growth Promoting Bacteria.- Chapter 17 Production of Volatile Organic Compounds in Plant Growth Promoting Rhizobacteria.- Chapter 18 Stress Physiology in Azospirillum and other Plant Growth Promoting Rhizobacteria.- Part III Biotic Interactions Toolbox.- Chapter 18 Alleviation of Abiotic and Biotic Stresses in Plants by Azospirillum.- Chapter 19 Interaction of Azospirillum spp. with Microalgae: a Basic Eukaryotic–Prokaryotic Model and its Biotechnological Applications.- Chapter 20 Pseudomonas and Azospirillum.- Chapter 21 Co–inoculation of Legumes with Azospirillum and Symbiotic Rhizobia.- Chapter 22 Interaction of Azospirillum and Mycorrhiza.- Part IV Agronomic and Industrial Applications Toolbox.- Chapter 23 Field Evaluation of Extensive Crops Inoculated with Azospirillum sp.- Chapter 24 Azospirillum spp. and Related Plant Growth Promoting Rhizobacteria Inocula Use in Intensive Agriculture.- Chapter 25 Inoculant Preparation and Formulations for Azospirillum spp.- Chapter 26 Protocol for the Quality Control of Azospirillum spp. Inoculants.

O autorze

Dr. Fabricio Cassan, Author Universidad Nacional de Rio Cuarto Laboratorio de Fisiologia Vegetal y de la interacción Planta-microorganismo Ruta 36. Km 601 Rio Cuarto Cordoba, Argentina Cecilia M. Creus, Co-author Professor Adjunto Facultad de Ciencias Agrarias Universidad Nacional de Mar del Prata, INTA Balcarce.