Hairy Root Cultures Based Applications: Methods and Protocols

ISBN-13: 9789811540578 / Angielski / Miękka / 2021 / 237 str.

Vikas Srivastava; Shakti Mehrotra; Sonal Mishra

Spis treści

Part A: Establishment of hairy root cultures and / or native production of Secondary Metabolites

1. Establishment of hairy root cultures for the production of Tropane alkaloids

2. Establishment of hairy root cultures for the production of (mono / di / sesqui) Terpene alkaloids

3. Hairy root cultures for saponins / triterpenoid saponins

4. Production of of Benzene isoquinolidine / pyrolizidine alkaloids from Hairy root cultures

5. Establishment of hairy root cultures for the production of Phenolics (flavonoids / quinones) compounds

Part B: Production strategies: Case studies of hairy root cultures for enhanced metabolite production  

6. Optimization of culture conditions for enhanced metabolite production through hairy root cultures

7. Influence of elicitation (physical / chemical/ biotic) on metabolite production in hairy root cultures

8. Improvement of metabolite production in hairy roots using Metabolic engineering / pathway modulation

9. Up scaling of hairy root cultures in bioreactors for enhanced biomass and proportionate metabolite production

Part C: Hairy roots as a tool for additional value applications

10. Production of valuable molecules through bioconversion of exogenous substrate using hairy root cultures

11. Utilization of hairy root cultures for Antibody / vaccine production

12. Potential of hairy root cultures for the Remediation of toxic compounds

Part D: Application of hairy root research: emerging trends

13. Mathematical / computational simulation in growth of hairy root cultures

14. Involvement of Crisper/Cas9 for genome editing in hairy root cultures

15. Functional characterization in hairy root cultures (plant-microbe interaction)

16. Agrobacterium rhizogenes mediated hairy root cultures of parasite plants to study host- parasite plant relationship

O autorze

Dr. Vikas Srivastava is an Assistant Professor at the Department of Botany, Central University of Jammu, India. He completed his Ph.D. at Lucknow University, India, and his post-doc at the National Institute of Plant Genome Research (NIPGR), New Delhi, India. Specializing in various areas of biotechnology, he has training and experience in diverse fields of plant biology. He has published numerous articles in respected international journals/books of repute, and has been working as Principal Investigator for a major project sponsored by the University Grant Commission (UGC), New Delhi. He is a recipient of various awards and prestigious fellowships, and has delivered several invited lectures at leading national institutes.

Dr. Shakti Mehrotra is a consulting scientist at the Department of Biotechnology, Institute of Engineering and Technology, Dr. Abdul Kalam Technical University, Lucknow, India. She completed her Ph.D. at Lucknow University, India, and post-doc at the Institute of Engineering and Technology, Lucknow (DBT-PDF) and CSIR-CIMAP (DSTYoung Scientist). Specializing in various areas of biotechnology, she has training and experience in diverse fields of plant biotechnology. She has published numerous articles in respected national and international journals/books and worked as Principal Investigator for major projects sponsored by the Department of Science and Technology (DST) and Department of Biotechnology (DBT), Government of India. She is a recipient of various awards and prestigious fellowships. She has delivered several presentations at conferences and workshops.

Dr. Sonal Mishra is currently working as a Dr. D. S. Kothari-Post Doctoral Fellow (UGC-DSKPDF) at the School of Biotechnology, University of Jammu, India. She has recently completed a SERB-National Post Doc Fellowship (SERB-NPDF) as Principal Investigator at the School of Biotechnology, University of Jammu, India. Further, she completed a post-doc at Jawaharlal Nehru University and National Institute of Plant Genome Research (NIPGR), India. She pursued her Ph.D. jointly from the Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP, Lucknow) and Jawaharlal Nehru University, New Delhi. Specializing in various areas of biotechnology, she has training and experience in diverse fields of plant biotechnology and molecular biology. She has published numerous articles in respected international journals/books. She is a recipient of various awards and prestigious fellowships, and has presented her work in at seminars and conferences.

Blurb

The book discusses the various methods and protocols available in hairy root culture-based research. The utilization of Agrobacterium mediated genetic transformation and establishment of hairy root cultures has paved the way for large-scale secondary metabolite production in medicinal plants. Presenting recent research and offering insights from eminent research groups, the book covers a range of topics related to hairy root-based applications, including (i) establishment of hairy roots and native production of SM (ii) yield enhancement strategies for increased SM production, like elicitation (iii) hairy roots as a tool for value-added applications such as plant-microbe interaction, characterization of plant genes and root biology studies. As such it is an informative guide and experimental manual for researchers in diverse fields of plant biology.