Haemostasis: Methods and Protocols

ISBN-13: 9781493961023 / Angielski / Miękka / 2016 / 431 str.

Paul Monagle

Opis

This Methods in Molecular Biology (TM) book reviews the major components of the haemostatic system, general principles of haemostatic testing and protocols for assessing various aspects of the haemostatic system, grouped according to their functional indications.

Spis treści

Part I. The Haemostatic System

 

1. The Coagulation System in Humans

            Anthony K. C. Chan and Nethnapha Paredes

 

2. Platelet Physiology

            Matthew D. Linden

 

3. The Role of the Blood Vessel Wall

            Nethnapha Paredes and Anthony K. C. Chan

 

Part II. General Principles of Haemostatic Testing

 

4. Specimen Requirements for the Haemostasis Laboratory

            Linda Stang and Lesley G. Mitchell

 

5. Principles of Haemostasis Tests

            Gemma Crighton

 

6. Reference Ranges of Coagulation Tests

            Oliver Speer, Markus Schmugge, Claudia Metzger, and Manuela Albisetti

 

7. Lupus Anticoagulant Testing

            Karen Moffat, Anne Raby, and Mark Crowther

 

 

Part III. Examples of Global Tests of Haemostasis

 

8. Activated Partial Thromboplastin Time (APTT)

            Vera Ignjatovic

 

9. Prothrombin Time (PT) and International Normalised Ratio (INR)

            Vera Ignjatovic

 

10. Thrombin Clotting Time (TCT)

            Vera Ignjatovic

 

11. Thrombin Generation

            Leslie R. Berry and Anthony K. C. Chan

 

12.  Activated Clotting Time (ACT)

            Stephen Horton and Simon Augustin

 

 

Part IV. Examples of Tests for Bleeding Disorders

 

13. Factor XII Assays

            Stephen Opat, Jenny Butler, Erica Malan, Elizabeth Duncan, and Huyen A.M. Tran

 

14. Fibrinogen

            Linda Stang and Lesley G. Mitchell

 

15. Platelet Counting

            Sylvain Salignac, Véronique Latger-Cannard, Nicole Schlegel, and Thomas Lecompte

 

16. Platelet Morphology Analysis

            Véronique Latger-Cannard, Odile Fenneteau, Sylvain Salignac, Thomas Lecompte, and Nicole Schlegel

 

17. Light Transmission Aggregometry

            Juan Pablo Frontroth

 

18. Platelet Flow Cytometry

            Matthew D. Linden

 

 

Part V. Examples of Specific Assays Related to Anticoagulant Activity

 

19. Anti-factor Xa assay (anti-Xa)

            Fiona Newall

 

20. Reptilase Time (RT)

            Hratsch Karapetian

 

21. Protamine Titration

            Fiona Newall

 

22. Laboratory Methods for the Assay if Tissue Factor Pathway Inhibitor (TFPI) in Human Plasma

            Robyn Summerhayes

 

23. Heparin Induced Thrombocytopenia (HIT)

            Andreas Greinacher, Birgitt Fürll, and Sixten Selleng

 

Part VI. Examples of Tests for Inhibitors of Coagulation

 

24. Nijmegen-Bethesda Assay to Measure Factor VIII Inhibitors

            Elizabeth Duncan, Margaret Collecutt, and Alison Street

 

25. Kaolin Clotting Time

            Kottayam Radhakrishnan

 

26. The Dilute Russell Viper Venom Time (DRVVT)

            Kottayam Radhakrishnan

 

27. Platelet Neutralisation Test

            Kottayam Radhakrishnan

 

Part VII. Examples of Tests for Increased Risk of Thrombosis

 

28. Antithrombin

            Mirta Hepner and Vasiliko Karlaftis

 

29. Protein C

            Mirta Hepner and Vasiliki Karlaftis

 

30. Protein S

            Mirta Hepner and Vasiliki Karlaftis

 

31. Testing for Hyperhomocysteinemia in Subjects with a History of  Thromboembolic Events Using HPLC Technique

            Jonas Denecke

 

32. Anticardiolipin Antibody and Anti-Beta 2 Glycoprotetin I Assays

            Anne Raby, Karen Moffat, and Mark Crowther

 

33. Testing for Apolipoprotein (a) Phenotype Using Isoelectric Focusing and  Immunoblotting Technique

            Claus Langer, Bertram Tambyrayah, and Ulrike Nowak-Göttl

 

Part VIII. Examples of Measures of Fibrinolysis

 

34. D-Dimer and Fibrinogen/Fibrin Degradation Products

            Linda Stang

Blurb

The haemostatic system is one the most important physiological systems for maintaining health and well being, and thus the investigation of the haemostatic system remains a research priority. Disturbances of the haemostatic system in the broader sense, such as heart disease and strokes, arguably constitute the single greatest contribution to non-infectious mortality in the world today. Therefore, understanding the laboratory methods to assess the haemostatic system is vital for the practice of complex clinical medicine. In Haemostasis: Methods and Protocols, experts in the field address the major components of the haemostatic system, general principles of haemostatic testing, and techniques used to assess various aspects of the haemostatic system, grouped according to their functional indications. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Haemostasis: Methods and Protocols provides an ideal guide to scientists of all backgrounds and serves an urgent need for further research to develop superior methods of assessing the haemostatic system in humans.