Glycovirology Protocols

ISBN-13: 9781617376641 / Angielski / Miękka / 2010 / 206 str.

Richard J. Sugrue

Opis

This volume reviews the increasing importance of glycosylation to the field of virology, as well as virus replication. The chapters provide an overview of glycosylation in relation to virus infection, and the generic techniques that are used to analyze and characterize glycoproteins.
Among the topics discussed in this volume are M.E. interaction between respiratory syncytial virus and glycosaminoglycans, acute respiratory syndrome coronavirus 3a protein, the C type lectins DC-SIGN and L-SIGN, N-linked glycans, and the use of two-dimensional SDS-PAGE to analyze the glycosylation heterogeneity of the respiratory syncytial virus fusion protein. Also covered in this volume are uses of monoclonal antibodies and lectins, the spike glycoprotein, secretion of respiratory syncytial vius F protein from insect cells, the expression and characterization of recombinant dengue virus E protein, and cloning, expression, and a functional analysis of patient-derived hepatitis C virus glycoproteins.
The information presented in Glycovirology Protocols will provide insight as to how the techniques of glycobiology can be applied in virology and answer most questions that are of interest to the reader.

Spis treści

Table of Contents Chapter 1: Viruses and glycosylation: an overview -- Sugrue, R.J. Chapter 2: M.E. Interaction between Respiratory Syncytial Virus and Glycosaminoglycans, including Heparan Sulfate. -- Hallak, L.K., Kwilas, S.A. and Peeples, Chapter 3: Expression of the severe acute respiratory syndrome coronavirus 3a protein and the assembly of coronavirus-like particles in the Baculovirus expression system -- Khan, S., Ng, M.L. and Tan,Y.J. Chapter 4: The C type lectins DC-SIGN and L-SIGN: receptors for viral glycoproteins -- Lozach, P.Y., Burleigh, L., Staropoli, I. and Amara. A. Chapter 5: Functional analysis of the N-linked glycans within the fusion protein of respiratory syncytial virus -- Li, P. Rixon, H.W.McL., Brown, G. and Sugrue, R.J. Chapter 6: Expression and purification of viral glycoproteins using recombinant Vaccinia Viruses for functional and structural Studies -- Li, Z.N. and Steinhauer, D.A. Chapter 7: The use of 2-dimensional SDS PAGE to analyse the glycosylation heterogeneity of the respiratory syncytial virus (RSV) Fusion (F) Protein -- McDonald, T.P. and Sugrue, R.J. Chapter 8: The use of monoclonal antibodies and lectins to identify changes in viral glycoproteins that are influenced by gltcosylation: The case of respiratory syncytial virus attachment (G) glycoprotein-- Rawling, J. and Melero, J.A. Chapter 9: Expression, glycosylation and modification of the spike (S) glycoprotein of severe acute respiratory syndrome coronavirus (SARS CoV) -- Shen, S., Tan, T.H.P. and Tan, Y.J. Chapter 10: Analysis of glycoproteins of viruses in the family Bunyaviridae -- Shi, X. and Elliott, R.M. Chapter 11: Secretion of respiratory syncytial virus F protein from insect cells -- Tan, B.H., Brown, G. and Sugrue, R.J. Chapter 12: The expression and characterisation of recombinant dengue virus E protein expressed in Pichia pastoris. -- Tan, B.H., Fu, J.L. and Sugrue, R.J. Chapter 13: Cloning, expression and functional analysis of patient-derived hepatitis C virus glycoproteins -- Tarr, A.W., Owsianka, A.M., Szwejk, A., Ball, K.K. and Patel, A.H.