Glycolipids: Methods and Protocols

ISBN-13: 9781071629093 / Angielski / Twarda / 2023 / 302 str.

Kazuya Kabayama; Jin-Ichi Inokuchi

Opis

This detailed volume presents a variety of specialized analytical methods on a wide range of glycolipids. From sphingoglycolipids to lipid A, an immune system stimulant present in lipopolysaccharide, the book delves into how glycolipid structure on the surface of the cell membrane, which characterizes each organism, is crucial for the coexistence or repulsion between organisms. In addition, glycolipids play an important role in infections in animals and other organisms by targeting host cell membranes, if enveloped viruses are considered as living organisms. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Glycolipids: Methods and Protocols serves as an ideal guide to the most advanced glycolipid research in an effort to help researchers make their research more fruitful.

Spis treści

1. Immunomodulatory Functions of α-GalCer and a Derivative, α-Carba-GalCer

            Masashi Satoh and Kazuya Iwabuchi

 

2. Chemical Synthesis and Molecular Interaction Analysis of a-Galactosyl Ceramide Derivatives as CD1d Ligands

            Kazunari Ueki, Kodai Sueyoshi, Shinsuke Inuki, and Yukari Fujimoto

 

3. Immunomodulatory Functions of Glycolipids from Pathogens

            Carla Guenther, Miyuki, Watanabe, and Sho Yamasaki

 

4. Chemical Synthesis and Immunomodulatory Functions of Bacterial Lipid As

            Atsushi Shimoyama and Koichi Fukase

 

5. Innovative Vaccine Strategy: Self-Adjuvanting Conjugate Vaccines

            Yoshiyuki Manabe and Koichi Fukase

 

6. Chemical Synthesis of Phosphatidylglucoside

            Koki Kano, Nozomi Ishii, Peter Greimel, and Ichiro Matsuo

 

7. Sialidase-Resistant Ganglioside GM3 Analogues: Evaluation of Biological Activity

            Go Hirai

 

8. Chemical Synthesis of Gangliosides

            Akihiro Imamura, Hiromune Ando, and Hideharu Ishida

 

9. Sialyltransferase Activity Assay for Ganglioside GM3 Synthase

            Kei-ichiro Inamori, Takahiro Nitta, Fumi Shishido, Soichiro Watanabe, Isao Ohno, and Jin-ichi Inokuchi

 

10. Construction of Sphingolipid Remodeled Cells by Genome Editing

            Toshiyuki Yamaji and Yuta Homma

 

11. Mass Spectrometry of Neutral Glycosphingolipids

            Akemi Suzuki, Atit Silsirivanit, Takashi Watanabe, Junko Matsuda, Kei-ichiro Inamori, and Jin-ichi Inokuchi

 

12. Structural Analyses of the Glycolipids in Lipid Rafts

            Yusuke Suzuki and Kazuya Kabayama

 

13. Extraction, Purification, and Chemical Degradation of LPS from Gut Microbiota Strains

            Molly Dorothy Pither, Alba Silipo, Antonio Molinaro, and Flaviana Di Lorenzo

 

14. NMR Analysis of Mammalian Glycolipids

            Yoshiki Yamaguchi

 

15. Distribution of Glycolipids in the Plasma Membrane Monitored by Specific Probes in Combination with Sodium Dodecyl Sulfate-Digested Freeze-Fracture Replica Labeling (SDS-FRL)

            Motohide Murate and Toshihide Kobayashi

 

16. Biochemical and Microscopic Analyses for Sphingolipids and Its Related Molecules in Phagosomes

            Hitoshi Nakayama, Kei Hanafusa, and Kazuhisa Iwabuchi

 

17. Single-Molecule Imaging of Ganglioside Probes in Living Cell Plasma Membranes

            Kenichi G.N. Suzuki

 

18. Assays and Utilization of Enzymes Involved in Glycolipid Metabolism in Bacteria and Fungi

            Makoto Ito, Yohei Ishibashi, Takashi Watanabe, Jun Iwaki, Toyohisa Kurita, and Nozomu Okino

 

19. Molecular Dynamics of Glycolipids in Liposomes

            Tomokazu Yasuda, J. Peter Slotte, Michio Murata, and Shinya Hanashima

 

20. Inhibitors of Glucosylceramide Synthase

            James A. Shayman, Vania Hinkovska-Galcheva, and Liming Shu

 

21. Comprehensive Glycan Analysis of Sphingolipids in Human Serum/Plasma

            Hisatoshi Hanamatsu, Takashi Nishikaze, and Jun-ichi Furukawa