Glutamate Receptors: Methods and Protocols

ISBN-13: 9781493990764 / Angielski / Twarda / 2019 / 260 str.

Burger, Corinna

Opis

This volume aims to provide clear and detailed methods to probe glutamate receptor function. Chapters in this book feature methods to study synaptic ultrastructure, receptor dynamics/receptor interactions and trafficking, cellular plasticity, receptor gene regulation, epigenetics, and clinical applications. In addition, it includes overview chapters that provide critical reviews and historical perspectives on particular techniques and fields of study. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Glutamate Receptors: Methods and Protocols covers well-established glutamate receptor techniques, as well as new and cutting-edge techniques designed so that students, researchers, and clinicians can gain an understanding of the issues surrounding each experimental technique and its potential applications.

Spis treści

Preface…
Table of Contents…
Contributing Authors…

Part I Synaptic Ultrastructure/Receptor Dynamics/Receptor Interactions and Trafficking 

1. Single Molecule FRET Methods to Study Glutamate Receptors
Douglas Litwin, Ryan Durham, and Vasanthi Jayaraman

2. Ultrastructural Observation of Glutamatergic Synapses by Focused Ion Beam Scanning Electron Microscopy (FIB/SEM)
Ai Takahashi, Laxmi Kumar Parajuli, Hirohide Iwasaki, Shinji Tanaka, and Shigeo Okabe

3. Considerations for Imaging and Analyzing Neural Structures by STED Microscopy
Martin O. Lenz and Jan Tønnesen

4. Cell-Based Enzyme-Linked Immunosorbent Assay (Cell-ELISA) Analysis of Native and Recombinant Glutamate Receptors
Elek Molnár

Part II Function/Cellular Plasticity

5. Preparation of Organotypic Slice Cultures for the Study of Glutamate Receptor Function
Andres Barria

6. Glutamate Receptor Probing with Rapid Application and Solution Exchange (RASE)
Nathanael O’Neil and Sergiy Sylantyev

7. Electrophysiological Investigation of Metabotropic Glutamate Receptor-Dependent Metaplasticity in the Hippocampus
Regina U. Hegemann and Wickliffe C. Abraham

8. Induction of Metabotropic Glutamate Receptor-Mediated Long-Term Depression in the Hippocampal Schaffer Collateral Pathway of Aging Rats
Kirstan E. Gimse, Kenneth O’Riordan, and Corinna Burger

9. Whole-Cell Patch Clamp Electrophysiology to Study Inotropic Glutamatergic Receptors and Their Roles in Addiction
Jonna M. Leyrer-Jackson, M. Foster Olive, and Cassandra D. Gipson 

Part III Receptor Gene Regulation/Epigenetics

10. Gene Expression Analysis by Multiplex Single Cell RT-PCR
Tricoire Ludovic, Cauli Bruno, and Lambolez Bertrand

11. Metabolomics Analysis of Glutamate Receptor Function
Nataliya E. Chorna and Anatoliy P. Chornyy

12. Locus-Specific DNA Methylation Assays to Study Glutamate Receptor Regulation
Jordan A. Brown, J. David Sweatt, and Garrett A. Kaas

13. Preparation of Synaptoneurosomes for the Study of Glutamate Receptor Function
Cara J. Westmark and Pamela R. Westmark

Part VI Clinical Applications

14. Fractionation of Subcellular Compartments from Human Brain Tissue
Toni M. Mueller, Pitna Kim, and James H. Meador-Woodruff

15. Glutamate Receptor Antibodies in Autoimmune Central Nervous System Disease: Basic Mechanisms, Clinical Features, and Antibody Detection
WJ Scotton, Abid Karim, and Saiju Jacob