Glucose Transport: Methods and Protocols

ISBN-13: 9781493975068 / Angielski / Twarda / 2017 / 274 str.

Karin Lindkvist; Jesper Hansen

Spis treści

Preface…

Table of Contents…

Contributing Authors…

1 Expression and Purification of Rat Glucose Transporter 1 

in Pichia pastoris 

Raminta Venskutonytė, Karin Elbing, and Karin Lindkvist-Petersson

2 Crystallization and Structural Determination of the Human Glucose Transporters GLUT1 and GLUT3

Dong Deng and Nieng Yan

3 Screening and Scale-Up of GLUT Transporter Constructs Suitable for Biochemical and Structural Studies

Grégory Verdon, Hae Joo Kang, and David Drew

4 GLUT Characterization using Frog Xenopus laevis Oocytes

Wentong Long, Debbie O'Neill, and Chris I. Cheeseman

5 Glucose Uptake in Heterologous Expression Systems

Eunice E. Lee and Richard C. Wang

6 Evaluating the Impact of GLUT Inhibition on Cellular Bioenergetics Using a Seahorse XF Analyzer

Changyong Wei, Monique Heitmeir, Paul W. Hruz, and Mala Shanmugam

7 Glucose Transport Activity Measured in Giant Vesicles

Jesper Søndergaard Hansen and Karin Lindkvist-Petersson

8 Design, Synthesis, and Evaluation of GLUT Inhibitors

Carlotta Granchi, Tiziano Tuccinardi, and Filippo Minutolo

9 Applying Microfluidic Systems to Study Effects of Glucose at Single Cell Level

Niek Welkenhuysen, Caroline B. Adiels, Mattias Goksör, and Stefan Hohmann

10 A Growth-Based Screening System for Hexose Transporters in Yeast

Eckhard Boles and Mislav Oreb

11 Identification of Insulin Activated Rab Proteins in Adipose Cells Using Bio-ATB-GTP Photolabeling Technique

Francoise Koumanov and Geoffrey D. Holman

12 Total Internal Reflection Fluorescence Microscopy to Study GLUT4 Trafficking

Sebastian Wasserstrom, Björn Morén, and Karin G. Stenkula

13 Translocation and Redistribution of GLUT4 Using a Dual-Labeled Reporter Assay

Robert M. Jackson and Ann Louise Olson

14 GLUT4 Translocation in Single Muscle Cells in Culture: Epitope Detection by Immunofluorescence

Javier R. Jaldin-Fincati, Philip J. Bilan, and Amira Klip

15 Glucose Transport: Methods for Interrogating GLUT4 Trafficking in Adipocytes

Dougall M. Norris, Tom A. Geddes, David E. James, Daniel J. Fazakerley, and James G. Burchfield

16 Proximity Ligation Assay to Study the GLUT4 Membrane Trafficking Machinery

Dimitrios Kioumourtzoglou, Gwyn W. Gould, and Nia J. Bryant

17 Quantification of Cell Surface Glucose Transporters in the Heart Using a Biotinylated Photolabeled Assay

Zahra Maria and Veronique A. Lacombe

18 Tracking GLUT2 Translocation by Live Cell Imaging

Sabina Tsytkin-Kirschenzweig, Merav Cohen, and Yaakov Nahmias

19 GLUT2-Expressing Neurons as Glucose Sensors in the Brain: Electrophysiological Analysis

Gwenaël Labouèbe, Bernard Thorens, and Christophe Lamy

Blurb

This volume explores methods used to examine the structure-function relationship of glucose transporters both in vitro and ex vivo. Chapters in this book cover topics such as expression and purification of glucose transporters in heterologous expression systems, crystal structure determination, glucose transport activity assays in cellular and synthetic model systems, investigation of the GLUT4 membrane trafficking machinery by novel labeling strategies combined with advanced fluorescence microscopy methods, and tracking GLUT2 translocation by live cell imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Glucose Transport: Methods and Protocols is a valuable resource that helps researchers with determining specific roles of different GLUTs in various organisms.