Glaucoma: Methods and Protocols

ISBN-13: 9781493984763 / Angielski / Miękka / 2018 / 337 str.

Tatjana C. Jakobs

Spis treści

1. Use of Animal Models and Techniques in Glaucoma Research: Introduction

            Harry A. Quigley

 

2. Hypertonic Saline Injection Model of Experimental Glaucoma in Rats

            John C. Morrison, Elaine C. Johnson, and William O. Cepurna

 

3. The Microbead Occlusion Model of Ocular Hypertension in Mice

            David J. Calkins, Wendi S. Lambert, Cathryn R. Formichella, William M. McLaughlin, and Rebecca M. Sappington

 

4. Ocular Hypertension/Glaucoma in Minipigs: Episcleral Veins Cauterization and Microbead Occlusion Methods

            Elena Vecino, Haritz Urcola, and Sansar C. Sharma

 

5. Non-Invasive Intraocular Pressure Measurement in Animals Models of Glaucoma

            Yan Hu and John Danias

 

6. High Throughput Binocular Pattern Electroretinograms in the Mouse

            Tsung-Han Chou, Jonathon Toft-Nielsen, and Vittorio Porciatti

 

7. Visual Evoked Potentials in Glaucoma and Alzheimer’s Disease

            Elisa Cerri, Carlotta Fabiani, Chiara Criscuolo, and Luciano Domenici

 

8. Investigation of the Functional Retinal Output Using Microelectrode Arrays

            Günther Zeck

 

9. Quantitative Proteomic Analysis of Human Aqueous Humor Using iTRAQ 4plex Labeling

            Anna Trzeciecka, Padmanabhan Pattabiraman, Maria Carmen Piqueras, Carol Toris, and Sanjoy K. Bhattacharya

 

10. Sphingolipid Analysis of Aqueous Humor

            Anna Trzeciecka, Arturo Barron, Genea Edwards, Sruthi Sampathkumar, Carol Toris, and Sanjoy K. Bhattacharya

 

11. Assessment of Aqueous Humor Dynamics in the Rodent by Constant Flow Infusion

            J. Cameron Millar, Tien N. Phan, and Iok-Hou Pang

 

12. Methods for Analyzing Endoplasmic Reticulum Stress in the Trabecular Meshwork of Glaucoma Models

            Prabhavathi Maddineni, Ramesh B. Kasetti, and Gulab S. Zode

 

13. Quantification of Scleral Biomechanics and Collagen Fiber Alignment

            Ian C. Campbell, Joseph M. Sherwood, Darryl R. Overby, Bailey G. Hannon, A. Thomas Read, Julia Raykin, and C. Ross Ethier

 

14. Biolistic Labeling of Retinal Ganglion Cells

            Luca Della Santina and Yvonne Ou

 

15. Anterograde Tract Tracing for Assaying Axonopathy and Transport Deficits in Glaucoma

            Samuel D. Crish and Brett R. Schofield

 

16. In Vitro and In Vivo Methods for Studying Retinal Ganglion Cell Survival and Optic Nerve Regeneration

            Yuqin Yin and Larry I. Benowitz

 

17. 3D Histomorphometric Reconstruction and Quantification of the Optic Nerve Head Connective Tissues

            Hongli Yang, Juan Reynaud, Howard Lockwood, Galen Williams, Christy Hardin, Luke Reyes, Stuart Gardiner, and Claude F. Burgoyne

 

18. Visualizing Astrocytes of the Optic Nerve

            Daniel Sun

 

19. Investigation of MicroRNA Expression in Experimental Glaucoma

            Hari Jayaram, Diana C. Lozano, Elaine C. Johnson, and John C. Morrison

 

20. Utilizing RNA-Seq to Identify Differentially Expressed Genes in Glaucoma Model Tissues, Such as the Rodent Optic Nerve Head

            Diana C. Lozano, Dongseok Choi, Hari Jayaram, John C. Morrison, and Elaine C. Johnson

 

21. Single-Cell Dissociation and Characterization in the Murine Retina and Optic Nerve

            Hee Joo Choi, Rui Wang, and Tatjana C. Jakobs

Blurb

This detailed volume introduces the reader to current methods in glaucoma research, in particular those that make use of animal disease models. Given that the retinal ganglion cells damaged and destroyed by glaucoma do not regenerate after lowering the intraocular pressure, new therapeutic approaches that protect ganglion cells directly is a vital research goal that this volume’s contents aim to aid scientists in developing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Glaucoma: Methods and Protocols serves as a useful guide for both ophthalmologists in clinical practice and researchers in the field.