Germline Stem Cells

ISBN-13: 9781603272131 / Angielski / Twarda / 2008 / 273 str.

Steven X. Hou; Shree RAM Singh

Opis

Stem cells are responsible for maintaining tissue homeostasis. They possess unlimited self-renewal potency. Through asymmetric cell division, a stem cell in adult tissues can produce an offspring that will maintain the stem cell populations and a daughter cell that will differentiate into various short-lived cell types to replace damaged or dying cells. Tumors may originate from a few transformed cancer stem cells. Stem cells have immense potential for therapeutic use in regenerative medicine and for developing anticancer therapies that specifically eliminate cancer stem cells. Germline stem cells are the source of human and animal reproduction. The knowledge gained from studying germline stem cells may find immediate application in preserving endangered wildlife, managing commercial livestock, overcoming fertility problems in humans, and treating testicular and ovary tumors. To make use of this potential, we have to learn how to isolate, characterize, and maintain germline stem cells. To isolate, characterize, and maintain stem cells, we must first understand the molecular parameters that define a germline stem cell and the mechanisms that regulate stem cell behavior. The protocols in Germline Stem Cells are intended to present selected genetic, molecular, and cellular techniques used in germline stem cell research. The book is divided into two parts. Part I covers germline stem cell identification and regulation in model organisms. Part II covers current techniques used in in vitro culture and applications of germline stem cells.

Recenzje

From the reviews:

"This book provides background information and protocols for identifying, isolating, characterizing, and manipulating ... germline stem cells. ... This book is written for basic science and clinical researchers ... as well as those interested in development and reproductive biology. Biomedical scientists and clinicians ... will also appreciate the wealth of new information. ... This is an outstanding contribution to the literature in germ cell biology. ... will be valued by all those interested in development of the germ lineage and stem cell biology." (Bruce A. Fenderson, Doody's Review Service, October, 2008)

Spis treści

Contents Preface……………………………………………………………………………….. Contributors………………………………………………………………………….. Part I Identification and Regulation of Germline Stem Cells in Model Organisms The Development of Germline Stem Cells in Drosophila David A. Dansereau and Paul Lasko………………………………………...... Analysis of the C. elegans Germline Stem Cell Region Sarah L. Crittenden and Judith Kimble ………………………………………. Immunohistological Techniques for Studying the Drosophila Male Germline Stem Cell Shree Ram Singh and Steven X. Hou…………………………………………. Genetic Tools Used for Cell Lineage Tracing and Gene Manipulation in Drosophila Germline Stem Cells Wei Liu and Steven X. Hou…………………………………………………… Structural Polarity and Dynamics of Male Germline Stem Cells in an Insect (the milkweed bug Oncopeltus fasciatus) David C. Dorn and August Dorn……………………………………………… High Resolution Light Microscopic Characterization of Spermatogonia Hélio Chiarini-Garcia and Marvin L Meistrich……………………………….. Identification and Characterization of Spermatogonial Subtypes and Their Expansion in Whole Mounts and Tissue Sections from Primate Testes Jens Ehmcke and Stefan Schlatt........................................................................... Epigenetic Control in Male Germ Cells Durga Prasad Mishra and PaoloSassone-Corsi………………………………. GDNF Maintains Mouse Spermatogonial Stem Cells in vivo and in vitro Hannu Sariola and Tiina Immonen…………………………………………….. Part II In vitro Culture and Applications of Germline Stem Cells Ectopic Grafting of Mammalian Testis Tissue into Mouse Hosts Ina Dobrinski and Rahul Rathi……………………………………… Spermatogonial Stem Cell Transplantation, Testicular Function and Restoration of Male Fertility in Mice Derek J. McLean…………………………………………………………………... 12 Isolating Highly Pure Rat Spermatogonial Stem Cells in Culture F. Kent Hamra, Karen M. Chapman, Zhuoru Wu, and David L. Garbers…chrically Complex Trait Jason D. Heaney and Joseph H. Nadeau………………………………………… Study Origin of Germ Cells and Formation of New Primary Follicles in Adult Human and Rat Ovaries Antonin Bukovsky, Satish K. Gupta, Irma Virant-Klun, Nirmala B. Upadhyaya, Pleas Copas, Stuart E. Van Meter, Marta Svetlikova, Maria E. Ayala, and Roberto Dominguez …………………………………………………….. Index………………………………………………………………………………………..

Blurb

The knowledge gained by studying germline stem cells, the source of human and animal reproduction, may find immediate application in preserving endangered wildlife, managing commercial livestock, overcoming fertility problems in humans, and treating testicular and ovary tumors.  In Germline Stem Cells, leading experts explore the parameters that define germline stem cells and the mechanisms that regulate the cell behavior in order to better isolate, characterize and maintain them.  Divided into two parts, the volume begins by providing protocols for germline stem cell identification and regulation in model organisms, and concludes with detailed chapters covering current techniques involving in vitro culture and the applications of the cells.  Following the highly successful Methods in Molecular Biology™ series format, each chapter includes a brief introduction to the subject, a list of necessary equipment and reagents, step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Germline Stem Cells is the perfect reference for scientists exploring this particular exciting and important subdivision in the fast-paced field of stem cell research.