Germline Stem Cells

ISBN-13: 9781493940158 / Angielski / Twarda / 2016 / 229 str.

Michael Buszczak

Opis

This second edition provides updated and new chapters on selected genetic, molecular, biochemical, and cell biological techniques. Chapter's guide readers through methods and principles on primordial germ cells and germline stem cells, however many of these principles can be applied to different types of adult stem cells.

Spis treści

Sarah L. Crittenden, Hannah S. Seidel, and Judith Kimble

 

2.  Methods for Studying the Germline of the Human Parasite Schistosoma  Mansoni

Julie N.R. Collins and James J. Collins III

 

3.  Evaluation of the Asymmetric Division of Drosophila Male Germline Stem Cells

Mayu Inaba and Yukiko M. Yamashita

 

4.  Live Imaging of the Drosophila Testis Stem Cell Niche

Leah J. Greenspan and Erika L. Matunis

 

5.  RNA Isolation from Early Drosophila Larval Ovaries

Dana Gancz and Lilach Gilboa

 

7.  Measurement Of Mrna Poly(A) Tail Lengths In Drosophila Female Germ Cells and Germline Stem Cells

Aymeric Chartier, Willy Joly, and Martine Simonelig

 

13.  Mouse Fetal Germ Cell Isolation and Culture Techniques

Cassy M. Spiller, Guillaume Burnet, and Josephine Bowles

 

14.  Rattus Norvegicus Spermatogenesis Colony Forming Assays

F. Kent Hamra

15.  Identification of Mouse Pirna Pathway Components Using Anti-MIWI2 Antibodies

Takamasa Hirano, Hidetoshi Hasuwa, and Haruhiko Siomi

Blurb

This second edition provides updated and new chapters on selected genetic, molecular, biochemical, and cell biological techniques. Chapter’s guide readers through methods and principles on primordial germ cells and germline stem cells, however many of these principles can be applied to different types of adult stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Germline Stem Cells: Second Edition aims to present the new findings and techniques that have developed since the first edition.