Germline Development in the Zebrafish: Methods and Protocols

ISBN-13: 9781071609729 / Angielski / Miękka / 2022

Dosch, Roland

Opis

This volume details a wide range of methods, ranging from beginner through advanced, used tofurther study zebrafish and fish germline.. Chapter guide readers through cultivating and manipulating germ cells, imaging of germline processes and the molecular analysis of their, protein, and RNA.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Germline Development in the Zebrafish: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Spis treści

1. Separation of Oocyte and Follicle Layer for Gene Expression Analysis in Zebrafish

Nana Ai, Lin Liu, Esther Shuk-Wa Lau, Anna Chung-Kwan Tse, and Wei Ge

 

2. The Xenopus Oocyte as an Expression System for Functional Analyses of Fish Aquaporins

François Chauvigné, Alba Ferré, and Joan Cerdà

 

3. Computer-Assisted Sperm Analysis to Test Environmental Toxicants

Tímea Kollár, Ákos Horváth , and Zsolt Csenki-Bakos

 

4. Cryopreservation and Transplantation of Spermatogonial Stem Cells

Zoran Marinović, Jelena Lujić, Qian Li, Yoshiko Iwasaki, Béla Urbányi, Goro Yoshizaki, and Ákos Horváth

 

5. Masculinization of Zebrafish through Partial Depletion of Primordial Germ Cells by Injecting Diluted Morpholino Oligonucleotides into Embryos

László Orbán, Jolly M. Saju, Keh-Weei Tzung, and Woei Chang Liew

 

6. Chemical Genetics: Manipulating the Germline with Small Molecules

Youngnam N. Jin and Randall T. Peterson

 

7. In vitro Induction of Teleost PGCs

Vanesa Robles, David G. Valcarce, and Marta F. Riesco

 

8. Applying Rho Pathway Inhibitors to Investigate Germ Plasm Localization

Jerónimo Miranda and Denhí Schnabel

 

9. Cryopreservation of Pooled Sperm Samples

Jennifer L. Matthews and Zoltan M. Varga

 

10. Quantifying Tissue Tension in the Granulosa Layer after Laser Surgery

Peng Xia and Carl-Philipp Heisenberg

 

11. In-vivo and Ex-vivo CT Imaging of Zebrafish Gonads

Christian Dullin and Louisa Habich

 

12. Live and Time-lapse Imaging of Early Oogenesis and Meiotic Chromosomal Dynamics in Cultured Juvenile Zebrafish Ovaries

Avishag Mytlis and Yaniv M. Elkouby

 

13. Detection of the Polar Body after Fertilization

Hua Ruan, Xiaogui Yi and Honghui Huang

 

14. Labelling the Micropylar Cell in Zebrafish Whole-mount and Cryo-sectioned Follicles

Chaitanya Dingare, Petra A. Klemmt, Alina Niedzwetzki,Virginie Lecaudey

 

15. Visualisation of Transcriptional Activity in Early Zebrafish Primordial Germ Cells

Fabio M. D’Orazio, Aleksandra Jasiulewicz, Yavor Hadzhiev, and Ferenc Müller

 

16. Experimental Methodologies to Visualise Early Cell Biology in Zebrafish Embryos

Asfa Sabrin Borbor and Sreelaja Nair                                                     

 

17. Observation of Medaka Larval Gonads by Immunohistochemistry and Confocal Laser Microscopy

Toshiya Nishimura and Minoru Tanaka

 

18. Methods for Visualization of RNA and Cytoskeletal Elements In The Early Zebrafish Embryo
Hansen, C.L. and  Pelegri, F.

 

19. Glyoxal Fixation as an Alternative for Zebrafish Embryo Immunostaining

Nadia Rostam and Roland Dosch

 

20. Histological Analysis of Gonads in Zebrafish

Kellee R. Siegfried and Jocelyn S. Steinfeld

 

21. Manipulating and Visualizing the Germline with Transgenic Lines

Ding Ye and Yonghua Sun

 

22. Liquid Chromatography and Tandem Mass Spectrometry in Label Free Protein Quantification of Zebrafish (Danio rerio) Eggs

Ozlem Yilmaz, Emmanuelle Com, Regis Lavigne, Charles Pineau, and Julien Bobe

 

23. In vivo Protein Lifetime Measurements across Multiple Organs in the Zebrafish

Sunit Mandad, Gudrun Kracht, and Eugenio F. Fornasiero

 

24. In vivo Imaging of Protein Interactions in the Germ Plasm with Bimolecular Fluorescent Complementation

Roshan Perera and Roland Dosch

 

25. The Zygotic Genome Activation: Critical Prelude to the Most Important Time of Your Life

Vladimir Korzh

 

26. Identification of RNA-binding Protein Landscapes Across Zebrafish Embryonic Transcriptome via iCLIP Approach

Vladimir Despic

 

27. Tethered Function Assay to Study RNA Regulatory Proteins in Zebrafish Embryos

Yuichiro Mishima and Kunio Inoue

 

28. Massively Parallel Analysis of Regulatory RNA Sequences

Michal Rabani

 

29. Exploring Translational Control of Maternal mRNAs in Zebrafish

Cecilia Lanny Winata, Maciej Łapiński, Hisyam Ismail, Sinnakaruppan Mathavan, and Prabha Sampath