Genotype Phenotype Coupling: Methods and Protocols

ISBN-13: 9781071632789 / Angielski

Stefan Zielonka; Simon Krah

Spis treści

1. Quantitative Determination of Staphylococcus aureus Using Aptamer-Based Recognition and DNA Amplification Machinery

            Nandi Zhou and Rongfeng Cai

 

2. Construction of Synthetic VHH Libraries in Ribosome Display Format

            Audrey Guilbaud and Frédéric Pecorari

 

3. Isolation of Adhirons Specific for Plant Protoplast Membrane Biomarkers is Simplified by Phagemid Design

            Claudia D’Ercole and Ario de Marco

 

4. Facile One-Step Generation of Camelid VHH and Avian scFv Libraries for Phage Display by Golden Gate Cloning

            Christina Bauer, Elke Ciesielski, Lukas Pekar, Simon Krah, Lars Toleikis, Stefan Zielonka, and Carolin Sellmann

 

5. Identification of New Antibodies Targeting Tumor Cell Surface Antigens by Phage Display

            Steffen Krohn, Matthias Peipp, and Katja Klausz

 

6. Phage Display of Bovine Ultralong CDRH3

            Callum Joyce, Louise Speight, Alastair D.G. Lawson, Anthony Scott-Tucker, and Alex Macpherson

 

7. Bacterial Cell Display for Selection of Affibody Molecules

            Charles Dahlsson Leitao, Stefan Ståhl, and John Löfblom

 

8. Isolation of Antigen-Specific Unconventional Bovine Ultra-Long CDR3H Antibodies Using Cattle Immunization in Combination with Yeast Surface Display

            Paul Arras, Jasmin Zimmermann, Britta Lipinski, Desislava Yanakieva, Daniel Klewinghaus, Simon Krah, Harald Kolmar, Lukas Pekar, and Stefan Zielonka

 

9. Selection of High-Affinity Heterodimeric Antigen-Binding Fc Fragments from a Large Yeast Display Library

            Filippo Benedetti, Gerhard Stadlmayr, Katharina Stadlbauer, Florian Rüker, and Gordana Wozniak-Knopp

 

10. A Two-Step Golden Gate Cloning Procedure for the Generation of Natively Paired YSD Fab Libraries

            Lena Vollmer, Simon Krah, Stefan Zielonka, and Desislava Yanakieva

 

11. Single-Cell B-Cell Sequencing to Generate Natively-Paired scFab Yeast Surface Display Libraries

            Nathaniel Pascual, Theodore Belecciu, Sam Schmidt, Athar Nakisa, Xuefei Huang, and Daniel Woldring

 

12. One-Pot Droplet RT-OE-PCR for the Generation of Natively Paired Antibody Immune Libraries

            Desislava Yanakieva, Lena Vollmer, Satyendra Kumar, Stefan Becker, Lars Toleikis, Lukas Pekar, Harald Kolmar, Stefan Zielonka, and Simon Krah

 

13. Affinity Maturation of the Natural Ligand (B7-H6) for Natural Cytotoxicity Receptor NKp30 by Yeast Surface Display

            Stefan Zielonka, Simon Krah, Paul Arras, Britta Lipinski, Jasmin Zimmermann, Ammelie Svea Boje, Katja Klausz, Matthias Peipp, and Lukas Pekar

 

14. Accessing Transient Binding Pockets by Protein Engineering and Yeast Surface Display Screening

            Jorge A. Lerma Romero and Harald Kolmar

 

15. Tyrosine Phosphorylation Screening on the Yeast Surface by Magnetic Bead Selection and FACS

            Jose Ezagui and Lawrence A. Stern

 

16. Bulk Reformatting of Antibody Fragments Displayed on the Surface of Yeast Cells to Final IgG Format for Mammalian Production

            Stefania C. Carrara, Jan P. Bogen, David Fiebig, Julius Grzeschik, Björn Hock, and Harald Kolmar

 

17. Antibody Secreting Cell Isolation from Different Species for Microfluidic Antibody Hit Discovery

            Ramona Gaa, Qingyong Ji, and Achim Doerner

 

18. Efficient Microfluidics Downstream Processes for Rapid Antibody Hit Confirmation

            Ramona Gaa, Hannah Melina Mayer, Daniela Noack, and Achim Doerner

 

19. Cell Line Development Using Targeted Gene Integration into MAR-Rich Landing Pads for Stable Expression of Transgenes

            Claudia Oliviero, Steffen C. Hinz, Julius Grzeschik, Björn Hock, Harald Kolmar, and Gerrit Hagens

 

20. Generation of Human 293-F Suspension NGFR Knockout Cells Using CRISPR/Cas9 Coupled to Fluorescent Protein Expression

            Stefanie Schatz, Femke Harmina van Dijk, Aleksandra Elzbieta Dubiel, Tobias Cantz, Reto Eggenschwiler, and Jörn Stitz

 

21. Antibody Display Technology (ADbody) to Present Challenging and Unstable Target Proteins on Antibodies

            Fu-Lien Hsieh and Tao-Hsin Chang

 

22. SUMO: In Silico Sequence Assessment Using Multiple Optimization Parameters

            Andreas Evers, Shipra Malhotra, Wolf-Guido Bolick, Ahmad Najafian, Maria Borisovska, Shira Warszawski, Yves Fomekong Nanfack, Daniel Kuhn, Friedrich Rippmann, Alejandro Crespo, and Vanita Sood

 

23. Streamlined Data Analysis Pipeline for Deep Sequence-Coupled Biopanning Identification of Pathogen-Specific Antibody Responses in Serum

            Javier Leo, Susan B. Core, and Kathryn M. Frietze

Blurb

This detailed new edition broadens the scope of the first edition by moving beyond classical display technologies. This book explores methodologies for the generation of natively paired antibody libraries, single cell technologies, alternative scaffolds, and in silico antibody sequence assessments are described. The application of these methods may allow for a generation of improved therapeutics and diagnostic reagents in a shorter time frame. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Genotype Phenotype Coupling: Methods and Protocols, Second Edition serves as an ideal guide for researchers seeking to expand their knowledge of antibody-based therapeutics.