Genotoxicity Assessment: Methods and Protocols

ISBN-13: 9781493996452 / Angielski / Twarda / 2019 / 378 str.

Alok Dhawan; Mahima Bajpayee

Spis treści

Part I: Gene Mutation Assays

 

1. In Vitro Mouse Lymphoma Cell (L5178Y Tk^+/- -3.7.2.C) Forward Mutation Assay

            Melissa R. Schisler, Martha M. Moore, and B. Bhaskar Gollapudi

 

2. Erythrocyte-Based Pig-a Gene Mutation Assay

            Jeffrey C. Bemis, Nikki E. Hall, and Stephen D. Dertinger

 

3. Detection of In Vivo Mutation in the Hprt and Pig-a Genes of Rat Lymphocytes

            Vasily N. Dobrovolsky, Joseph G. Shaddock, Roberta A. Mittelstaedt, Daishiro Miura, and Robert H. Heflich

 

Part II: Assays for Chromosomal Abnormalities

 

4. In Vitro Cytogenetic Assays: Chromosomal Aberrations and Micronucleus Tests

            Pasquale Mosesso and Serena Cinelli

 

5. Dicentric Chromosome Assay (DCA) and Cytokinesis-Block Micronucleus (CBMN) Assay in the Field of Biological Dosimetry

            Antonella Testa, Valentina Palma, and Clarice Patrono

 

6. Chromosomal Aberration Test in Human Lymphocytes

            Christian Johannes and Guenter Obe

 

7. In Vivo Micronucleus Assay in Mouse Bone Marrow

            Abhishek Kumar Jain and Alok Kumar Pandey

 

8. Micronucleus Cytome Assays in Human Lymphocytes and Buccal Cells

            Claudia Bolognesi and Michael Fenech

 

9. Micronucleus Analysis by Flow Cytometry

            Azeddine Elhajouji and Pascal Stadelmann

 

10. Hen’s Egg Test for Micronucleus Induction (HET-MN)

            Kerstin Reisinger, Eva Dony, Thorsten Wolf, and Katrin Maul

 

11. Comparative Genomic Hybridization (CGH) in Genotoxicology

            Adi Baumgartner, Veronika Hartleb, and Jim D. Taylor

 

Part III: Tests for Primary DNA Damage

 

12. The Comet Assay: Assessment of In Vitro and In Vivo DNA Damage

            Mahima Bajpayee, Ashutosh Kumar, and Alok Dhawan

 

13. The Comet Assay in Human Biomonitoring

            Diana Anderson and Julian Laubenthal

 

14. The Comet Assay in Marine Animals

            Patrizia Guidi, Brett P. Lyons, and Giada Frenzilli

 

15. Multicolour Laser Scanning Confocal Immunofluorescence Microscopy of DNA Damage Response Biomarkers

            Julian Laubenthal, Michal R. Gdula, Alok Dhawan, and Diana Anderson

 

16. Detection of DNA Double Strand Breaks Using Pulse Field Gel Electrophoresis

            Vyom Sharma and Vishwa Mohan

 

17. Detection of UV-Induced Thymine Dimers

            Vipin Kumar Yadav, Poorwa Awasthi, and Amit Kumar

 

Part IV: Assays in Plants and Alternate Animal Models

 

18. Micronucleus Assay with Tetrad Cells of Tradescantia

            Miroslav Mišík, Clemens Pichler, Bernhard Rainer, Armen Nersesyan, Katarina Mišíková, and Siegfried Knasmueller

 

19. The Wing-Spot and the Comet Tests as Useful Assays for Detecting Genotoxicity in Drosophila

            Ricard Marcos and Erico R. Carmona

 

Part V: In Silico Testing for Genetic Toxicology

 

20. In Silico Approaches in Predictive Genetic Toxicology

            Meetali Sinha, Alok Dhawan, and Ramkrishnan Parthasarathi

Blurb

This current edition explores new tests for genotoxicity testing, along with other well-known techniques. This will further help in our understanding of the genotoxic effects of chemicals. The book has different sections dealing with various assays for gene mutation, chromosomal abnormalities, primary DNA damage, etc. It also delves into plant models, animals and their alternates, as well as in silico approaches for genetic toxicology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Genotoxicity Assessment: Methods and Protocols, Second Edition serves as a highly useful and ready resource for research students and scientists working in regulatory toxicology as well as biomedical, biochemical, and pharmaceutical sciences.