Genomics Protocols

ISBN-13: 9781617378188 / Angielski / Miękka / 2010 / 425 str.

Mike Starkey; Ramnath Elaswarapu

Opis

Since the first edition, published in 2001, genomics research has taken great strides. In this updated second edition, a team of expert researchers share the most current information in a field that has recently switched emphasis from gene identification to functional genomics and the characterization of genes and gene products. This volume approaches its subject with a broad perspective to supply its reader with a vital overview of genomics and its derivative fields.

Recenzje

From the reviews of the second edition:

"The main purpose is to present current protocols in genomic research, and the book provides a comprehensive update on this area of research ... . The book is written primarily for scientists in the field of genomic research. ... presents a good balance between the different aspects involved in the study of functional genomics. It also provides a number of updated protocols for practicing investigators in the field of genomic research. ... a valuable companion to all researchers in the field of genomic research." (Ruben Mestril, Doody's Review Service, January, 2009)

Spis treści

Contents Preface Contributors 1. Whole Genome Amplification with Phi29 DNA Polymerase to enable Genetic or Genomic Analysis of Samples of Low DNA Yield Kaisa Silander………………………………………………………………………… 2. Scanning for DNA variants by denaturant capillary electrophoresis Per O. Ekstrøm……………………………………………………………………….. 3. Identification of SNPs, or Mutations in Sequence Chromatograms Nicole Draper…………………………………………………………………………. 4. Beadarray based Genotyping Helen Butler and Jiannis Ragoussis……………………………………………… 5. Microsatellite-Based Candidate Gene Linkage Analysis Studies Cathryn Mellersh………………………………………………………………...…… 6. Full Complexity Genomic Hybridization on 60mer Oligonucleotide Microarrays for Array Comparative Genomic Hybridization (aCGH) Alexei Protopopov, Bin Feng, and Lynda Chin………………………………… 7. Detection of Copy Number Changes at Multiple Loci in DNA Prepared from Formalin Fixed, Paraffin-Embedded Tissue by Multiplex Ligation- Dependent Probe Amplification Minoru Takata………………………………………………………………………… 8. Application of Microarrays for DNA Methylation Profiling Axel Schumacher, Andreas Weinhäusl, and Arturas Petronis……………… 9. Genome-Wide Identification of Protein Binding Locations using Chromatin Immunoprecipitation coupled with Microarray Byung-Kwan Cho, Eric M. Knight, and Bernhard Ø. Palsson……………….. 10. Transcriptional Profiling of Small Samples in the CentralNervous System Stephen D. Ginsberg………………………………………………………………… 11. Quantitative Expression Profiling of RNA from Formalin-Fixed, Paraffin- Embedded Tissues using randomly assembled Bead Arrays Marina Bibikova, Joanne M. Yeakley, Jessica Wang-Rodriguez, and Jian-Bing Fan ……………………………………………………………………. 12. Expression Profiling of microRNAs in Cancer Cells: Technical Considerations Mouldy Sioud and Lina Cekaite………………………………………………….… 13. Identification of Disease Biomarkers by Profiling of Serum Proteins using SELDI-TOF Mass Spectrome Arsalan S. Haqqani, John F. Kelly and Danica B. Stanimirovic…………… 18. Using 2D-LC-MS/MS to identify Francisella tularensis peptides in extracts from an infected mouse macrophage cell line John Kelly and Wen Ding………………………………………………………… 19. Baculovirus Expression Vector Systems (BEVS): An Emerging Host for High- Throughput Eukaryotic Protein Expression Binesh Shrestha, Carol Smee, and Opher Gileadi …………………………. 20. Co-immunoprecipitation and proteomic analyses S. Fabio Falsone, Bernd Gesslbauer and Andreas J. Kungl………….….. 21. Tandem affinity purification combined with mass spectrometry to identify components of protein complexes Peter Kaiser, David Meierhofer, Xiaorong Wang and Lan Huang……………. 22. Mammalian Two-Hybrid Assay For Detecting Protein-Protein Interactions in vivo Runtao He and Xuguang Li…………………………………………………..…….. 23. Detection of Protein-Protein Interactions in Live Cells and Animals with Split Firefly Luciferase Protein Fragment

Blurb

Since the first edition, published in 2001, genomics research has taken great strides. In Genomics Protocols, 2nd Edition, a team of expert researchers share the most current information in a field that has recently switched emphasis from gene identification to functional genomics and the characterization of genes and gene products. This volume approaches its complex subject with a broad perspective to supply its reader with a vital overview of genomics and its derivative fields, with a focus on pivotal issues such as data analysis. Following the highly successful Methods in Molecular Biology™ series format, the chapters provide easy-to-follow laboratory protocols, lists of the necessary materials, and the excellent Notes section, which offers tips on troubleshooting and avoiding known pitfalls.

Expansive and current, Genomics Protocols, 2nd Edition is the perfect update for researchers working in this integral, growing field.