Genome Editing in Animals: Methods and Protocols

ISBN-13: 9781071630150 / Angielski / Twarda / 2023 / 477 str.

Izuho Hatada

Opis

This second edition provides new and updated protocols that can be used for generation of knockout animals. Chapters guide the reader through basic protocols for three genome editing technologies, target design tools, and specific protocols for each animal. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Genome Editing in Animals: Methods and Protocols, Second Edition aims to be a useful practical guide to researches to help further their study in this field. 

Spis treści

1. Construction and Evaluation of Zinc Finger Nucleases

Hiroshi Ochiai and Takashi Yamamoto

 

2. Updated overview of TALEN construction systems

Tetsushi Sakuma and Takashi Yamamoto  

 

3. CRISPR/Cas9

Izuho Hatada, Sumiyo Morita, and Takuro Horii

 

4 SNPD-CRISPR: Single Nucleotide Polymorphism-Distinguishable Repression or Enhancement of a Target Gene Expression by CRISPR System

Shohei Maruyama, Takashi Kusakabe, Xinyi Zou, Yoshiaki Kobayashi, Yoshimasa Asano, Qingbo S. Wang, and Kumiko Ui-Tei

 

5. Utilizing large functional and population genomics resources for CRISPR/Cas perturbation experiment design

Qingbo S. Wang, Kumiko Ui-Tei

 

6. Generation of genome-edited mice by cytoplasmic injection of CRISPR-Cas9 RNA

Takuro Horii and Izuho Hatada

 

7. Gene targeting in mouse embryonic stem cells via CRISPR/Cas9 ribonucleoprotein (RNP) mediated genome editing.

Manabu Ozawa, Chihiro Emori, and Masahito Ikawa

 

8. Generation of Knock-in Mouse by Genome Editing

Wataru Fujii

 

9. Introduction of genetic mutations into mice by Base Editor and Target-AID

Hiroki Sasaguri

 

10. Genome editing in mouse and rat by electroporation

Takehito Kaneko

 

11. Generation of floxed mice by sequential electroporation

Takuro Horii, Ryosuke Kobayashi, and Izuho Hatada

 

12. Efficient detection of flox mice using in vitro Cre recombination

Ryosuke Kobayashi, Takuro Horii, and Izuho Hatada

 

13. VCre/VloxP and SCre/SloxP as reliable site-specific recombination systems for genome engineering

Manabu Nakayama

 

14. Efficient CRISPR/Cas9-Assisted Knockin of Large DNA Donors by Pronuclear Microinjection During S-phase in Mouse Zygotes

Takaya Abe, Ken-ichi Inoue, and Hiroshi Kiyonari

 

15. Genome editing of murine liver hepatocytes by AAV vector-mediated expression of Cas9 in vivo

Yuji Kashiwakura and Tsukasa Ohmori

 

16. Non-Viral Ex Vivo Genome-Editing in Mouse Bona Fide Hematopoietic Stem Cells with CRISPR/Cas9

Hiromasa Hara1, Natsagdorj Munkh-Erdene, Suvd Byambaa, and Yutaka Hanazono

 

17. Genome editing of rat

Takehito Kaneko

 

18. A simple and efficient method for generating KO rats using in vitro fertilized oocytes

Kohtaro Morita, Arata Honda, and Masahide Asano

 

19. Editing the Genome of the Golden Hamster (Mesocricetus auratus)

Michiko Hirose, Toshiko Tomishima, and Atsuo Ogura

 

20. Gene targeting in rabbits: single-step generation of knockout rabbits by microinjection of CRISPR/Cas9 plasmids

Arata Honda

 

21. Genome Editing of Pig

Masahito Watanabe and Hiroshi Nagashima

 

22. GEEP method: an optimized electroporation-mediated gene-editing approach for establishment of knockout pig lines

Fuminori Tanihara, Maki Hirata, and Takeshige Otoi

 

23. Genome editing mediated by primordial germ cell in chicken

Jae Yong Han and Hong Jo Lee

 

24. CRISPR–Cas9-mediated genome modifications in zebrafish

Yusuke Kamachi and Atsuo Kawahara

 

25. Genome Editing of Medaka

Rie Hara, Satoshi Ansai, and Masato Kinoshita

 

26. CRISPR-Cas9 based functional analysis in amphibians; Xenopus laevis, Xenopus tropicalis, and Pleurodeles waltl

Miyuki Suzuki, Midori Iida, Toshinori Hayashi, and Ken-ichi T Suzuki

 

27. Genome Editing of Silkworms

Takuya Tsubota, Hiroki Sakai, and Hideki Sezutsu

 

28. Improved genome editing in the ascidian Ciona with CRISPR/Cas9 and TALEN

Yasunori Sasakura and Takeo Horie

 

29. Genome editing of C. elegans

Takuma Sugi