Genome Editing

ISBN-13: 9783031080746 / Angielski / Miękka / 2023

Opis

Over the last few decades, various techniques have been developed to alter the properties of plants and animals.  While the targeted transfer of recombinant DNA into crop plants remains a valuable tool to achieve a desirable breeding outcome, integration of transgenes into the host genome has been random, which in part, leads to reduced acceptance of GMOs by the general population in some parts of the world. Likewise, methods of induced mutagenesis, such as TILLING, have the disadvantage that many mutations are induced per plant, which has to be removed again by expensive backcrossing. Advances in genome sequencing have provided more and more information on differences between susceptible and resistant varieties, which can now be directly targeted and modified using CRISPR/Cas9 technology. By selecting specific gRNAs occurrence of off-target modifications are comparatively low. ZFNs and TALENs- based approaches required re-engineering a new set of assembled polypeptides for every new target site for each experiment. The difficulty in cloning and protein engineering prevented these tools from being broadly adopted by the scientific community.  Compared to these technologies, designing the CRISPR toolbox is much simpler and more flexible. CRISPR/Cas9 is versatile, less expensive and highly efficient. It has become the most widely used technology for genome editing in many organisms.

Since its inception as a powerful genome-editing tool in late 2012, this breakthrough technology has completely changed how science is performed. The first few chapters in this book introduce the basic concept, design and implementation of CRISPR/Cas9 for different plant systems. They are followed by in-depth discussions on the legal and bio-safety issues accompanying commercialization and patenting of this emerging technology. Lastly, this book covers emerging areas of new tools and potential applications. We believe readers, novice and expert alike, will benefit from this all-in-one resource on genome editing for crop improvement.

Chapter 17 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Spis treści

S.No

Tentative Chapter Title

Author Details

1.  

Genome engineering as a tool for

enhancing crop traits: Lessons from

CRISPR/Cas9

Tariq Shah and Aziz Khan*

College of Agriculture, Guangxi University, Nanning, China

Email: azizkhanturlandi@gmail.com

 

1.  

Commercialization of CRISPR derived Crops: Current Status and future prospects

Dr. Martin A Lema   

Universidad Nacional de Quilmes, Roque Sáenz Peña 180, B1876BXD Bernal, Buenos Aires, Argentina. Email: mlema@unq.edu.ar

1.  

Targeted allele recombination for precise mapping in plant breeding

Dr. Eyal Fridman

Department of vegetables and field crops, Institute of Plant Science, Agricultural Research Organization, The Volcani Center, Rishon LeZion 7505101, Israel

Email: fridmane@volcani.agri.gov.il

Hindex: 28

1.  

Applications of gene drive for weeds and pest management using CRISPR/CAS9 system in plants

Dr. Kathleen Hefferon

 Food Science and Technology, College of Agriculture and Life Sciences, Cornell University, Ithaca, NY, USA

Email: klh22@cornell.edu

Hindex: 14

1.  

Genome editing by Ribonucleorotein  based delivery of CAS9 system  in plants

Karina Morales, Michael J. Thomson

Professor and HM Beachell Endowed Chair in International Rice Improvement

Dept. of Soil and Crop Sciences, Texas A&M University / Texas A&M AgriLife Research

370 Olsen Blvd., 2474 TAMU, College Station, TX 77843-2474

Email: email: m.thomson@tamu.edu

Hindex: 28

1.  

Virus mediated delivery of CRISPR/CAS9  system in plants

Dr. Monika Bansal,

Women scientist-A (completed)

School of Agricultural biotechnology,

Punjab Agricultural university, Ludhiana

Dr. Shabir Hussain Wani  

Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, J&K, India 

Email:shabirhwani@skuastkashmir.ac.in

Hindex: 24

 

Epigenetic and chromosomal Rearrangements in genome editing

Neha Goel (Ph.D.)

Genetics and Tree Improvement

Forest Research Institute, Dehradun

Email:goelneha6@gmail.con

Dr. Shabir Hussain Wani  

Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, J&K, India 

Email:shabirhwani@skuastkashmir.ac.in

Hindex: 24

1.  

Multiplexed genome editing in plants for  improvement of various traits using CRISPR/CAS9 based system

Dr. Goetz Hensel

Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)

Plant Reproductive Biology

Corrensstr. 3

D-06466 Seeland/OT Gatersleben

GERMANY

Email: hensel@ipk-gatersleben.de

Hindex: 33

1.  

Application of CRISPR CAS9 for regulation of epigenetic traits in plants

Abdullah Makhzoum

Department of Biological Sciences and Biotechnology, Botswana International University of Science and Technology, Palapye, Botswana; Correspondenceabmakhzoum@gmail.com

Hindex: 10

1.  

New Cas9 Variants and other nucleases broadening the scope of CRISPR Toolbox

Dr. Goetz Hensel

Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)

Plant Reproductive Biology

Corrensstr. 3

D-06466 Seeland/OT Gatersleben

GERMANY

Email: hensel@ipk-gatersleben.de

Hindex: 33

1.  

Transgene free genome editing in plants using CRISPR/CAS9 Dr. Niaz Ahmad Dr. Penny Hundleby

Department of Crop Genetics, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK

Email: penny.hundleby@jic.ac.uk

Prof. Mehboob Rahman

Group Leader Plant genomics & Mol. Breeding Lab, National Institute for Biotechnology & Genetic Engineering (NIBGE), PO Box 577, Jhang Road, Faisalabad, Pakistan.

Email: mehboob_pbd@yahoo.com

H index 23

 

Genome editing in plants for improvement of resistance in plants against fungal and viral pathogens

Dr. Kathleen Hefferon

 Food Science and Technology, College of Agriculture and Life Sciences, Cornell University, Ithaca, NY, USA

Email: klh22@cornell.edu

H index 14

 

1.  

Characterization of gene edited crops via metabolomics.

Dr. Muhammad Qudrat Ullah Farooqi

School of Agriculture and Environment; Faculty of Science

The University of Western Australia, Perth WA 6009 Australia

Email: muhammadqudratullah.farooqi@uwa.edu.au

 

1.  

Genome editing in plants for improvement of resistance in plants against bacterial pathogen

Dr. Rajesh Mehrotra

Birla Institute of Technology and Science (BITS-Pilani), Rahashthan, India

Email:

rmehrotra@pilani.bits-pilani.ac.in

 

Dr. Purvalohan Bhalothia

Email: purvalohan@gmail.com

 

H index 16

1.  

Improvement of resistance in plants against Insect-pest using genome editing tools

Dr Sandeep Kumar,

Assistant Professor,

Department of Zoology,

KU SSJ Campus, Almora, Uttarakhand,India

Email: sandeep.ento@gmail.com

 

Comparing the efficiency of different delivery methods of CRISPR/CAS9 toolkits into plants

Dr Ananda Sarkar

National Institute of Plant Genome Research (NIPGR), Jawaharlal Nehru University Campus, Aruna Asaf Ali Marg, New Delhi, Delhi, 110067, India

Email:aksarkar@nipgr.ac.in

 

Hindex 17

 

1.  

Recent trends in targeting genome editing of tomato for abiotic stress tolerance Dr. P. Hima Kumari,  Jawaharlal Nehru Technological University, Hyderabad, 500085

phimakumari@gmail.com

 

1.  

Genome editing approaches for improving nitrogen use efficiency and deciphering mineral nutrient homeostasis Viswanathan Chinnusamy Principal Scientist & Head

Division  of Plant Physiology

ICAR-Indian Agricultural Research Institute, New Delhi, India

Email: viswa_iari@hotmail.com

Hindex : 37

1.  

Recent advances and application of CRISPR base editors for improvement of various traits in crops

Dr Seema Pradhan and Dr Ajay Parida,

Director,

Institute of Life Sciences, Bhubaneswar, India

Email: drajayparida@gmail.com, director@ils.res.in

Dr.  Seema Pradhan

Email: seemapradhan3@gmail.com

 

1.  

CRISPR/Cas9-mediated targeted mutagenesis in medicinal plants Dr. Rohit Joshi Division of Biotechnology, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, 176061, India

Email: joshirohit6@gmail.com

H-index: 17

1.  

Biosafety issue related to genome editing in plants using CRISPR-Cas9 Dr. Ramesh Katam Department of Biological Sciences, Florida A&M University, Tallahassee, FL 32307, USA Email: ramesh.katam@gmail.com

1. 22

IPR and regulatory issues in Genome editing in plants.

Dimple Sharma

Plant and Soil Science department

Texas Tech University, USA

 Email: dimple.sharma@ttu.edu

 

 

O autorze

Dr. Shabir Hussain Wani received his PhD in Genetics and Plant Breeding on Development of Transgenic Rice for Drought and Salt Tolerance from Punjab Agricultural University. He has published more than 130 peer-reviewed papers and edited 20 books on plant stress physiology, including 10 with Springer. He is on Editorial Boards of Plant Cell Tissue and Organ Culture, Frontiers in Plant Science and Frontiers in Genetics. He is currently an Assistant Professor (Senior Scale), Genetics and Plant Breeding, at the Mountain Research Centre for Field Crops of the Sher-e-Kashmir University of Agricultural, Sciences and Technology of Kashmir in India.

Dr.  Goetz Hensel is a the Head of the Centre for Plant Genome Engineering at Heinrich-Heine-University Düsseldorf, Germany. Before, he was a Senior Researcher at the Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Germany. He is on the Editorial Boards of BMC Plant Biology, Frontiers in Plant Science, Plant Cell Tissue and Organ Culture, and the Journal of Applied Genetics. He is member of the Management Board of the Society for Plant Biotechnology, Germany. He serves in the EFB Plant, Agriculture and Food Division Board and the German Joint Synthetic Biology Working Group. He was involved in the improvement of methods of Agrobacterium-mediated gene transfer to cereals and in a new principle of RNA-mediated downregulation of genes called host-induced gene silencing (HIGS). He recently focused his research on designer endonuclease-mediated gene targeting (TALEN, CRISPR/Cas9), molecular farming and genes involved in the spike architecture and domestication of barley. He has published over 100 articles in this field.