1. Development of cellulolytic strain by genetic engineering approach for enhanced cellulose production 2. Development of cellulolytic thermo tolerant fungal strain 3. Development of efficient strain for both pentose and hexose sugar fermentation by genetic engineering approach 4. Development of ethanol fermenting yeast for high tolerance to inhibitory compounds 5. Development of efficient fungal strain for high enzyme production by metabolic engineering approach 6. Genetic engineering of cellulolytic fungal strain for ethanol production 7. Present status and future prospect of genetic and metabolic engineering for biofuel production from lignocellulosic biomass 8. Current status of cellulase production by fungal strains 9. Bioreactor design for efficient biofuel production from lignocellulosic biomass 10. Downstream processing of biofuel 11. Future scope and potential of genetic and metabolic engineering approach for cost effective biomass-based biofuel production