Genetic Toxicology: Principles and Methods

ISBN-13: 9781617794209 / Angielski / Twarda / 2011 / 433 str.

James M. Parry; Elizabeth M. Parry

Opis

The evaluation of potential mutagenic activity is a critical step in the assessment of the safety of both new and pre-existing chemical types. In Genetic Toxicology: Principles and Methods, expert contributors help to satisfy the demand for education in this tremendously important area of study. The volume covers three basic areas: the scientific basis of the discipline, the methodologies of the main test assays, and the application of the methods, all aimed primarily at scientists in the safety departments of the industries working with both natural and synthetic chemicals. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Intuitive and cutting-edge, Genetic Toxicology: Principles and Methods provides crucial support to both laboratory workers in providing quality information on the appropriate application of techniques and to study directors in their assay selection and protocol design in this vital field.

Spis treści

1. The Application of Structure Activity Relationships to the Prediction of the Mutagenic Activity of Chemicals

            Philip Judson

 

2. Bacterial Mutagenicity Assays: Test Methods

            David Gatehouse

 

3. The Mouse Lymphoma Assay

            Melvyn Lloyd and Darren Kidd

 

4. Mammalian Cell HPRT Gene Mutation Assay: Test Methods

            George E. Johnson

 

5. The In Vitro Mammalian Chromosome Aberration Test

            Gill Clare

 

6. The Interpretation and Analysis of Cytogenetic Data

            Natalie Danford

 

7. The In Vitro Micronucleus Assay

            Ann T. Doherty

 

8. The In Vitro and In Vivo Comet Assays

            Brian Burlinson

 

9. Assessment of DNA Interstrand Crosslinks (ICLs) Using the Modified Alkaline Comet Assay

            Jian Hong Wu and Nigel J. Jones

 

10. ³²P-Postlabelling for the Sensitive Detection of DNA Adducts

            Nigel J. Jones

 

11. Methods for the Detection of DNA Adducts

            Karen Brown

 

12. The GADD45a-GFP GreenScreen HC Assay

            Richard M. Walmsley and Matthew Tate

 

13. Real Time Reverse-Transcription Polymerase Chain Reaction (RT-PCR): Technical Considerations for Gene Expression Analysis

            Shareen H. Doak and Zoulikha M. Zaïr

 

14. Cytogenetic In Vivo Assays in Somatic Cells

            Ann T. Doherty, Adolph Baumgartner, and Diana Anderson

 

15. Cytogenetic Methods in Human Biomonitoring: Principles and Uses

            Raluca A. Mateuca, Ilsa Decordier, and Micheline Kirsch-Volders

 

16. The Measurement of Induced Genetic Change in Mammalian Germ Cells

            Ilse-Dore Adler, Francesca Pacchierotti, and Antonella Russo

 

17. Transgenic Animal Mutation Models: A Review of the Models and How They Function

            Steve Dean

 

18. Analysis of Genotoxicity Data in a Regulatory Context

            Ian de G. Mitchell and David O.F. Skibinski