Genetic Engineering: Principles and Methods

ISBN-13: 9781461570837 / Angielski / Miękka / 2012 / 340 str.

Jane K. Setlow

Opis

Genetic Engineering, Volume 24 contains discussions of contemporary and relevant topics in genetics, including: -Gene silencing: principles and applications, -Integrins and the myocardium, -Plant virus gene vectors: biotechnology and applications in agriculture and medicine, -Novel approaches to controlling transcription, -Use of DNA polymorphisms in genetic mapping, -Application of FLP/FRT site-specific DNA recombination system in plants. This principles and methods approach to genetics and genetic engineering is essential reading for all academics, bench scientists, and industry professionals wishing to take advantage of the latest and greatest in this continuously emerging field.

Spis treści

Genomic Footprinting.- Theoretical and Computer Analysis of Protein Primary Sequences: Structure Comparison and Prediction.- Affinity Chromatography of Sequence-Specific DNA-Binding Proteins.- Applications of the Firefly Luciferase as a Reporter Gene.- Fluorescence-Based Automated DNA Sequence Analysis.- Phosphorothioate-Based Oligonucleotide-Directed Mutagenesis.- Design and Use of Agrobacterium Transformation Vectors.- Cell Commitment and Determination in Plants.- Plasmids Derived from Epstein-Barr Virus: Mechanisms of Plasmid Maintenance and Applications in Molecular Biology.- Chromosome Jumping: A Long Range Cloning Technique.- Isolation of Intact mRNA and Construction of Full-Length cDNA Libraries: Use of a New Vector, ?gt22, and Primer-Adapters for Directional cDNA Cloning.- The Use of Transgenic Animal Techniques for Livestock Improvement.- Plant Reporter Genes: The Gus Gene Fusion System.- Structure of the Genes Encoding Proteins Involved in Blood Clotting.

O autorze

Jane K. Setlow, PhD. has championed the field of genetics for over 30 years, agreeing to edit Genetic Engineering: Principles & Methods since its inception in 1979. An internationally recognized scientist with numerous publications, Dr. Setlow is the former Chairperson of the NIH Recombinant DNA Molecule Advisory Committee (RAC). Her lab can found at Brookhaven National Laboratory, Upton, New York.