Genetic Engineering: Principles and Methods

ISBN-13: 9780306436161 / Angielski / Twarda / 1990 / 339 str.

J. K. Setlow;Setlow Jane Ed; Jane K. Setlow

Opis

Sixteen contributions cover such topics as the polymerase chain reaction; regulation of alternative splicing; human retinoblastoma susceptibility gene; control of translation initiation in mammalian cells; the utility of streptomycetes as hosts for gene cloning; folding of eukaryotic proteins produc

Spis treści

Folding of Eukaryotic Proteins Produced in EscherichiaColi.- Human Retinoblastoma Susceptibility Gene.- ?-Oligodeoxynucleotides (?-DNA): A New Chimeric Nucleic Acid Analog.- The Utility of Streptomycetes as Hosts for Gene Cloning.- From Footprint to Function: An Approach to Study Gene Expression and Regulatory Factors in Transgenic Plants.- Purification of Recombinant Proteins with Metal Chelate Adsorbent.- Determinants of Translation Efficiency of Specific mRNAs in Mammalian Cells.- The Polymerase Chain Reaction.- Regulation of Alternative Splicing.- Structure and Function of the Nuclear Receptor Superfamily for Steroid, Thyroid Hormone and Retinoic Acid.- Identification and Functional Analysis of Mammalian Splicing Factors.- The Genes Encoding Wheat Storage Proteins: Towards a Molecular Understanding of Bread-Making Quality and Its Genetic Manipulation.- Control of Translation Initiation in Mammalian Cells.- Electroporation of Bacteria: A General Approach to Genetic Transformation.- The Isolation and Identification of Cdna Genes by Their Heterologous Expression and Function.- Molecular Cloning of Genes Encoding Transcription Factors With the Use of Recognition Site Probes.

O autorze

Jane K. Setlow, PhD. has championed the field of genetics for over 30 years, agreeing to edit Genetic Engineering: Principles & Methods since its inception in 1979. An internationally recognized scientist with numerous publications, Dr. Setlow is the former Chairperson of the NIH Recombinant DNA Molecule Advisory Committee (RAC). Her lab can found at Brookhaven National Laboratory, Upton, New York.