Genetic Basis of Endometriosis : Understanding the Role of Tumor Suppressor Genes and Mitochondrial Genome in the Pathophysiology of Endometriosis

ISBN-13: 9783659863967 / Angielski / Miękka / 2016 / 236 str.

Suresh Govatati; Manjula Bhanoori; Shivaji Sisinthy

Opis

Endometriosis (MIM 131200) is a chronic gynecological disease characterized by the growth of endometrial tissue on ectopic sites. Histologically, endometriosis is a benign disease but exhibits several features similar to malignancy. Tumor suppressor gene (TSG) inactivation is likely to be involved in the proliferation and maintenance of endometriotic implants. Present study investigated loss of heterozygosity (LOH), alterations of TSGs and mitochondrial genome in endometriosis. PCR-GeneScan analysis revealed high LOH frequency at PTEN & TP53 loci. Sequencing of PTEN revealed 7 novel somatic mutations in endometriosis. CDH1 functional polymorphisms showed association with endometriosis. Expression studies revealed decreased expression of TP53, PTEN and BRCA1 and increased phosphorylation of Akt & BAD in endometriosis. Mitochondrial genome showed 51 somatic and 583 germ-line variants in endometriosis. The A13603G (Ser423Gly) novel missense mutation of ND5 gene showed 100% incidence in ectopic endometrium. Haplogroup M5 showed significant association with endometriosis. In conclusion, alterations of TSGs and mitochondrial genome are possible modulators of endometriosis risk.

O autorze

Dr Suresh has received PhD in Biochemistry from Osmania University and currently working as Postdoctoral Fellow at SKU, Anantapur, India. Dr Manjula is an eminent faculty member in the dept of Biochemistry, Osmania University. Dr Shivaji retired as director grade scientist from CCMB in 2012 & presently a research consultant at L V Prasad Eye Inst.