Gene Manipulation and Expression

ISBN-13: 9789401165679 / Angielski / Miękka / 2012 / 560 str.

Robert E. Glass

Opis

This book presents selected papers from the international symposium on "Gene Manipulation and Expression" held in the Bechyne Castle, in South Bohemia, Czechoslovakia, on September 3 to 7, 1984. The symposium was sponsored by Academician J.Riman, vicepresident of the Czechoslovak Academy of Sci- ences and director of the Institute of Molecular Genetics of the Czechoslovak Academy of Sciences, and by corresponding member of the Czechoslovak Academy of Sciences Prof.V.Krumphanzl, president of the Czechoslovak Society for Microbiology and director of the Institute of Microbiology of the Czechoslovak Academy of Sciences, with Dr.K.Mikulik of the same Institute acting as chairman of the Organizing Committee. The book is divided into four parts entitled: (l) Rearrangement of prokaryotic genetic material and DNA cloning vectors; (2) DNA-Dependent RNA polymerase in prokaryotes: structure and function; (3) The role of cAMP in gene expression; (4) Translational control of gene expression. We wish to thank all participants who con- tributed to this volume. We should also like to express our deep gratitude to Drs.I.Janda and J.Weiser, both of the Institute of Microbiology of the Czechoslovak Academy of Sciences, for their invaluable help in the editorial work and Mrs. J. Pistorova for patiently retyping all the manu- scripts.

Spis treści

One: Rearrangement of Prokaryotic Genetic Material and DNA Cloning Vectors.- 1 Genomic Structure and Evolution of Bacillus licheniformis ? and LP52 Phage Family.- 2 Expression of the Synthetic Proenkephalin Gene in E. coli.- 3 Cloning and Expression of Bacillus?-Glucanase Genes.- 4 Regulation of ?-Amylase Synthesis in Bacillus subtilis.- 5 New Variations on an Old Theme: Type I Restriction Enzymes and Their Recognition Sequences.- 6 The EcoDXX1 Restriction and Modification System of Escherichia coli ET7.- 7 Biological Function of Type I Restriction Enzymes.- 8 The Fosfomycin Resistance Transposon Tn2921.- 9 Transposition-like Events Mediated by Single-ended Derivatives of Transposon Tn21.- 10 Cloning and Expression of Determinants Encoding Toxigenicity in Enterobacteria.- 11 Extrachromosomal Replicons in Streptomyces lavendulaegrasserius.- Two: DNA-Dependent RNA Polymerase in Prokaryotes: Structure and Function, Promoter Selection.- 1 Genetic Dissection of E. coli RNA Polymerase.- 2 Non-essential Sequences in the ? Subunit of E. coli RNA Polymerase.- 3 Sequence Analysis of the ? Gene of E. coli RNA Polymerase: Correlation with Structure-Function Studies.- 4 DNA-Dependent RNA Polymerase from Streptomyces granaticolor.- 5 Transcriptional and Translational Signals in Phages PZA and ø29.- 6 Host-Vector System with the PR’ Promoter of Phage Lambda.- 7 On the Difficulties of Defining and Measuring Promoter Strength1 The Case of the Promoters of Bacterial rRNA Genes.- Three: The Role of cAMP in Gene Expression.- 1 Cyclic AMP in Bacteria: Catabolite Repression and Related Effects.- 2 DNA Sequences Involved in Expression and Regulation of deoR-, cytR- and cAMP/CRP-Controlled Genes in Escherichia coli.- 3 Differential Cellular Distribution of Cyclic AMP-Dependent Protein Kinase during Development of Dictyostelium discoideum.- 4 Amplification of the Adenylate Cyclase Gene in Escherichia coli K12.- 5 Factors Regulating the Activity of Escherichia coli Adenylate Cyclase.- 6 Regulation of Carbohydrate Metabolism by the Bacterial PEP:Sugar Phosphotransferase System.- 7 ATP-Dependent HPr Kinase Involved in Regulation of Carbohydrate Uptake in Gram-positive Bacteria: Inducer Exclusion and Inducer Expulsion.- 8 Regulation of cAMP Synthesis in Streptomyces granaticolor.- 9 Differences in Regulation of a Bacillus megaterium Metalloproteinase during Growth and Sporulation.- Four: Translational Control of Gene Expression.- 1 Ribosomal Protein Sl: “The Messenger RNA-Catching Arm” of Escherichia coli Ribosome.- 2 Initiation of Translation of Genetic Information in Streptomyces aureofaciens.- 3 Bacilli as Hosts for Protein Production.- 4 Structure and Expression of a Chloramphenicol Resistance Gene, cat-86, Cloned from Bacillus pumilus.- 5 An Approach to Controlled Removal of the 3? End of Ribosomal 16S RNA.- 6 Three Ribosomal tRNA-Binding Sites Involved in the Elongation Process.- 7 Structure, Function and Evolution of the 3? Ends of Small Ribosomal Subunit RNA.- 8 Amino Acid Residues In Elongation Factor Tu from Escherichia coli Near the Binding Site for the 3? Terminus of Aminoacyl-tRNA.- 9 Structural and Functional Alterations of Protein-Synthesis Elongation Factor Tu Purified from Streptomyces aureofaciens in an Aggregated State.- 10 Excessive Translational Accuracy Can Inhibit Growth.- 11 Particulate Protein-Synthesis Factors Associated with Translatable mRNA in Mammalian Cells.