Gene Isolation and Mapping Protocols

ISBN-13: 9780896033825 / Angielski / Miękka / 1996 / 318 str.

J. Boultwood; Jackie Boultwood; Jacqueline Boultwood

Opis

Over the past 10 years great progress has been made in the development of efficient techniques for both gene isolation and mapping. The identifica tion and isolation of transcribed sequences from large chromosomal regions are central to the human genome mapping project. Techniques for isolating novel cDNAs have applications both in the overall construction and integra tion of long-range physical and transcription maps and in the identification of disease genes. A number of different techniques for the isolation of cDNAs from mam malian genomes have been developed, including screening "zoo" blots, the use of large genomic clones (YACs or cosmids) for hybridization against cDNA libraries, and CpG island mapping. More recently two highly efficient tech niques have been introduced: exon trapping, based on the presence of exon splice sites, and direct selection, based on the enrichment of selected cDNAs using immobilized YACs or cosmids. Leading researchers in the field have contributed chapters detailing the practical procedures for these and other widely used methods. The most rapid progress presently being made in the field of gene isolation concerns the partial sequencing of cDNA clones from one or both ends to produce expressed sequence tags (ESTs). Indeed, by Octo ber 1995, the EST division of Genbank (dbEST) contained a total of approxi mately 270,000 human EST sequences accounting for almost half the number of sequence entries in Genbank.

Recenzje

...a useful addition to the libraries of individuals and institutions... Three Stars. - Doody's Health Sciences Book Review Journal

"...this series of excellent and detailed lab manuals....of general use to all those mapping and isolation genes."-SGM Quarterly

Spis treści

Gene Mapping Goes from FISH to Surfing the Net, John Valdes and Danilo A. Tagle. Linkage Analysis of Genetic Disorders, Eugene W. Taylor, Jianfeng Xu, Ethylin Wang Jabs, and Deborah A. Meyers. Gene Ordering and Localization by Linkage Analysis, R. E. March. Gene Mapping Using Somatic Cell Hybrids, David P. Kelsell and Nigel K. Spurr. Gene Mapping by FISH, Rafael Espinosa III and Michelle M. Le Beau. Probe Ordering and Distancing by FISH, Marian Kroef, Hans Dauwerse, and Jim Landegent. Physical Mapping by Pulsed-Field Gel Electrophoresis, John Maule. Construction and Use of YAC Contigs in Disease Regions, Stephen Horrigan and Carol Westbrook. Construction and Use of Cosmid Contigs, Nicholas Fairweather. Use of Dinucleotide Polymorphism Analyses in Physical Mapping, Jeff Fairman and Lalitha Nagarajan. Mapping Expressed Sequence Tags (ESTs) by Multiplexing PCR Reactions from Hybrid Cell Panels and Detecting Fluorescently Labeled Products, A. Scott Durkin, Donna R. Maglott, and William C. Nierman. Gene Isolation by Exon Trapping, David B. Krizman. Isolation of Coding Sequences from Genomic Regions Using Direct Selection, Richard G. Del Mastro and Michael Lovett. Isolation of cDNAs Using the YAC Hybridization Screen Method, Carrie Fidler and Jacqueline Boultwood. Detection and Isolation of Differentially Expressed Genes by Differential Display, Weimin Zhu and Peng Liang. Chemical Crosslinking Subtraction (CCLS), Ian N. Hampson and Lynne Hampson. Gene Mapping and Isolation: Access to Databases, Martin J. Bishop. Software for Genetic Linkage Analysis: An Update, Stephen P. Bryant. Exon Detection by Similarity Searches, Jean-Michel Claverie. Index.