Gene Expression Profiling: Methods and Protocols

ISBN-13: 9781617792885 / Angielski / Twarda / 2011 / 211 str.

Lorraine O'Driscoll

Opis

Understanding gene expression and how it changes under normal and pathological conditions is essential to our understanding of the fundamentals of cell biology through to the targeted treatment of disease. In Gene Expression Profiling: Methods and Protocols, Second Edition, experts in their particular fields compile detailed protocols for a broad range of techniques, currently available and being further developed, for the analysis of gene expression at the DNA, RNA, and protein levels. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and easy-to-use, Gene Expression Profiling: Methods and Protocols, Second Edition presents a collection of clearly described and illustrated chapters, certain to be helpful to researchers in academia, in hospitals, and in industry who are interested in applying techniques, whether basic or advanced, for the analysis of gene expression.

Recenzje

From the reviews of the second edition:

"This beautifully illustrated book on gene expression profiling provides detailed protocols for a broad range of techniques ... for the analysis of gene expression at the DNA, RNA, and protein levels. ... Although written for researchers, the book will be helpful for graduate students and others interested in learning about gene expression. ... This is a valuable resource on methods and protocols necessary to understand the changes in gene expression in normal and pathological states in pursuit of better treatments." (Omer Iqbal, Doody's Book Reviews, April, 2012)

Spis treści

1. Real-Time PCR and Multiplex Approaches

            Olga L. Gurvich and Mikhail Skoblov

 

2. Reverse-Transcriptase Polymerase Chain Reaction to Detect Extracellular mRNAs

            Sweta Rani and Lorraine O’Driscoll

 

3. Microarray Analysis of mRNAs: Experimental Design and Data Analysis Fundamentals

            Jai Prakash Mehta

 

4. Software and Tools for Microarray Data Analysis

            Jai Prakash Mehta and Sweta Rani

 

5. Analysis of Gene Expression as Relevant to Cancer Cells and Circulating Tumor Cells

            Anne M. Friel, John Crown, and Lorraine O’Driscoll

 

6. Gene Expression Profiling in Formalin-Fixed, Paraffin-Embedded Tissues Using the Whole-Genome DASL Assay

            Craig S. April and Jian-Bing Fan

 

7. MicroRNA Expression Analysis: Techniques Suitable for Studies of Intercellular and Extracellular MicroRNAs

            Erica Hennessy and Lorraine O’Driscoll

 

8. Western Blotting Analysis as a Tool to Study Receptor Tyrosine Kinases

            Serena Germano and Lorraine O’Driscoll

 

9. 2D Gel Electrophoresis and Mass Spectrometry Identification and Analysis of Proteins

            Paula Meleady

 

10. Design, Construction, and Analysis of Cell Line Arrays and Tissue Microarrays for Gene Expression Analysis

            Kathy Gately, Keith Kerr, and Ken O’Byrne

 

11. Immunohistochemical and Immunofluorescence Procedures for Protein Analysis

            Kishore Reddy Katikireddy and Finbarr O’Sullivan

 

12. Advanced Microscopy: Laser Scanning Confocal Microscopy

            Orla Hanrahan, James Harris, and Chris Egan

 

13. Isolation of Exosomes for Subsequent mRNA, MicroRNA, and Protein Profiling

            Sweta Rani, Keith O’Brien, Fergal C. Kelleher, Claire Corcoran, Serena Germano, Marek Radomski, John Crown, and Lorraine O’Driscoll

 

14. Atomic Force Microscopy (AFM) and High-Content Analysis (HCA): Two Innovative Technologies for Dissecting the Relationship Between Epithelial-Mesenchymal Transition (EMT)-Related Morphological and Structural Alterations and Cell Mechanical Properties

            Stephen T. Buckley, Anthony M. Davies, and Carsten Ehrhardt