Gene, Drug, and Tissue Engineering

ISBN-13: 9781071627150 / Angielski / Twarda / 2022 / 345 str.

Glaucia C. Pereira

Opis

This book combines discursive chapters that present the latest progress in molecular biology, drug discovery, organ-tissue engineering, and related fields, with a number of descriptive chapters on methods, protocols, and case studies. Structured into four parts, this volume walks the reader through the latest in cellular biology, with discussions on novel medicinal plant metabolites, nanotechnology in precision medicine, nucleic acid-based therapeutics and vaccines, genetic engineering, computational aid, bioinformatics, synthetic organs for transplantation, and organ-tissue engineering. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detail and expert implementation advice that ensures quality results in the lab. 

Authoritative and informative, Gene, Drug, and Tissue Engineering serves as an ideal guide for undergraduate students, postgraduate researchers, and senior researchers working in biomedicine and its underlying technologies, stimulating both computational and experimental development and fostering the exchange of new ideas.

Spis treści

Part I: Molecular Genetics and Genomics

 

1. Gene Alterations Induced by Glutamine (Q) Encoding CAG Repeats Associated with Neurodegeneration

            Gundappa Saha, Sukanya Ghosh, Vikash Kumar Dubey, and Prakash Saudagar

 

2. Latest Trends in Nucleic Acid-Based Engineering Techniques Applied to Precision Medicine

            Glaucia C. Pereira

 

3. Nanotechnology Driven Delivery Systems in Inoculation Therapies

            Glaucia C. Pereira

 

Part II: Molecular Biology in Drug Development and Therapeutics

 

4. Chronic Kidney Disease: Challenges in Translational Medicine

            Bruno Freire Botelho, André Luis Barreira, Maurilo Leite Jr., and Marcelo Marcos Morales

 

5. Mass Spectroscopy as an Analytical Tool to Harness the Production of Secondary Plant Metabolites: The Way Forward for Drug Discovery

            Faheem Ahmad and Hera Nadeem

 

6. Assessing the Challenges of Nanotechnology-Driven Targeted Therapies: Development of Magnetically Directed Vectors for Targeted Cancer Therapies and Beyond

            Matthew T. Bryan

 

Part III: Molecular, Cellular, Tissue Engineering, and AI-Based Computational Design

 

7. Advances in Regenerative Medicine and Biomaterials

            Şükran Şeker, Ayşe Eser Elçin, and Yaşar Murat Elçin

 

8. A Brief Review of Plant Cell Transfection, Gene Transcript Expression, and Genotypic Integration for Enhancing Compound Production

            Munish Sharma, Sakshi Bhushan, Deepak Sharma, Sanjana Kaul, and Manoj K. Dhar

 

9. A Review of Stem Cell Technology Targeting Hepatocyte Growth as an Alternative to Organ Transplantation

            Ernesto Goulart

 

10. Novel Nanotechnology-Driven Prototypes for AI-Enriched Implanted Prosthetics Following Organ Failure

            Glaucia C. Pereira

 

Part IV: Methods and Protocols

 

11. Simplified CRISPR-Mediated DNA Editing in Multicellular Eukaryotes

            Ritesh Kumar, Kartikeya Tiwari, and Prakash Saudagar

 

12. Design of SaCas9-HF for In Vivo Gene Therapy

            Kartikeya Tiwari, Ritesh Kumar, and Prakash Saudagar

 

13. Stem Cell Technology in Organ Transplantation: A Novel Method for 3D Bioprinting Functional and Stable Liver Grafts Using Human iPS-Derived Cells

            Ernesto Goulart

 

14. In Vitro Assay for the Assessment of Oxygen Depletion Triggers in Human Cell Lines, Associated with Improving Responses to Cancer Therapy

            Kartikeya Tiwari, Ritesh Kumar, Gundappa Saha, and Prakash Saudagar

 

15. The Use of ^99mTc-Labeled Stem Cells for Evaluation of Chronic Kidney Disease

            Bianca Gutfilen, Sergio Augusto Lopes de Souza, and Marcelo Marcos Morales

 

16. An Automated Strategy to Handle Antigenic Variability in Immunization Protocols, Part I: Nanopore Sequencing of Infectious Agent Variants

            Glaucia C. Pereira

 

17. An Automated Strategy to Handle Antigenic Variability in Immunization Protocols, Part II: In Vitro Transcribed mRNA Vector Design for Inoculation Against Infectious Agent Variants

            Glaucia C. Pereira