Gap Junction Protocols

ISBN-13: 9781493936625 / Angielski / Twarda / 2016 / 235 str.

Mathieu Vinken; Scott R. Johnstone

Opis

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Spis treści

1. Analysis of Liver Connexin Expression Using Reverse Transcription Quantitative Real-Time Polymerase Chain Reaction

            Michaël Maes, Joost Willebrords, Sara Crespo Yanguas, Bruno Cogliati, and Mathieu Vinken

 

2. DNA Methylation Analysis of Human Tissue-Specific Connexin Genes

            Xiaoming Liu and Canxia Xu

 

3. Detection of Connexins in Liver Cells Using Sodiumdodecylsulfate Polyacrylamide Gel Electrophoresis and Immunoblot Analysis

            Joost Willebrords, Michaël Maes, Sara Crespo Yanguas, Bruno Cogliati, and Mathieu Vinken

 

4. Immunohisto- and Cytochemistry Analysis of Connexins

            Bruno Cogliati, Michaël Maes, Isabel Veloso Alves Pereira, Joost Willebrords, Tereza Cristina da Silva, Sara Crespo Yanguas, and Mathieu Vinken

 

5. Small Interfering RNA-Mediated Connexin Gene Knockdown in Vascular Endothelial and Smooth Muscle Cells

            Miranda E. Good, Daniela Begandt, Leon J. DeLalio, Scott R. Johnstone, and Brant E. Isakson

 

6. Generation and Use of Trophoblast Stem Cells and Uterine Myocytes to Study the Role of Connexins for Pregnancy and Labor

            Mark Kibschull, Stephen J. Lye, and Oksana Shynlova

 

7. Identification of Connexin43 Phosphorylation and S-Nitrosylation in Cultured Primary Vascular Cells

            Alexander W. Lohman, Adam C. Straub, and Scott R. Johnstone

 

8. Preparation of Gap Junctions in Membrane Microdomains for Immunoprecipitation and Mass Spectrometry Interactome Analysis

            Stephanie Fowler, Mark Akins, and Steffany A.L. Bennett

 

9. Scrape Loading/Dye Transfer Assay

            Pavel Babica, Iva Sovadinová, and Brad L. Upham

 

10. Microinjection Technique for Assessment of Gap Junction Function

            Michael D. Fridman, Jun Liu, Yu Sun, and Robert M. Hamilton

 

11. Electroporation Loading and Dye Transfer: A Safe and Robust Method to Probe Gap Junctional Coupling

            Elke Decrock, Marijke De Bock, Diego De Baere, Delphine Hoorelbeke, Nan Wang, and Luc Leybaert

 

12. Using Fluorescence Recovery After Photobleaching to Study Gap Junctional Communication In Vitro

            Maria Kuzma-Kuzniarska, Clarence Yapp, and Philippa A. Hulley

 

13. Tracking Dynamic Gap Junctional Coupling in Live Cells by Local Photo-Activation and Fluorescence Imaging

            Song Yang and Wen-Hong Li

 

14. A Functional Assay to Assess Connexin43-Mediated Cell-to-Cell Communication of Second Messengers in Cultured Bone Cells

            Joseph P. Stains and Roberto Civitelli

 

15. Calcium Wave Propagation Triggered by Local Mechanical Stimulation as a Method for Studying Gap Junctions and Hemichannels

            Jegan Iyyathurai, Bernard Himpens, Geert Bultynck, and Catheleyne D'hondt

 

16. Establishment of the Dual Whole Cell Recording Patch Clamp Configuration for the Measurement of Gap Junction Conductance

            Richard D. Veenstra

Blurb

Presenting state-of-the-art protocols to study gap junctions, this detailed book first focuses on the use of methods and tools to investigate the different aspects of connexin expression and gap junction regulation. The second part of the volume describes several methods to probe gap junction functionality as such. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Gap Junction Protocols is intended for basic and applied researchers in the area of biomedical and life sciences, both in academic and industrial settings.