Fungal Antigens: Isolation, Purification, and Detection

ISBN-13: 9781461280750 / Angielski / Miękka / 2011 / 470 str.

Edouard Drouhet; Garry T. Cole; Louis De Repentigny

Opis

Three years ago when Professor Garry Cole visited our Mycology unit at the Pasteur Institute we discussed the possibility of organizing a small International Symposium on "Isolation, Purification and Detection of Fungal Antigens" limited to 8 American/Canadian scientists and to 8 French participants. The location chosen was the Pasteur Institute because of the historical and current importance of the Institute as a Center for Research in Immunology and Medical Mycology. The interest demonstrated by all medical mycolo gists we contacted led us to expand the small original meeting to an international symposium in which all aspects of antigens of pathogenic and allergenic fungi and actinomycetes related to man, animals, and even plants would be discussed. Our wish was also to hold this Symposium in the same week as the Anniversary meeting of the French Society of Medical Mycology which was founded at the Pasteur Institute 30 years ago with my colleagues Gabriel Segretain and Francois Mariat."

Spis treści

Overview on Fungal Antigens.- Methodology.- Chemical Methods to Study the Structure of Fungal Polysaccharide Antigens.- Cytochemical Techniques for the Study of Fungal Polysaccharides.- Immunochemistry of Fungal Antigens at the Electron Microscopy Level.- Enzyme-Linked Immunosorbent Assay and Related Sensitive Assays for Antigen and Antibody Detection.- Techniques Available to Prepare Monoclonal Antibodies Directed Toward Fungal Polysaccharide Antigens.- Fungal Exoantigens.- Invertebrate Pathogens.- Fungal Elicitors of Invertebrate Cell Defense System.- Candida.- Immunochemistry of Candida albicans Antigens.- Cytological, Chemical and Serological Immunodetection of a Candida albicans Antigen.- Candida Antigens and Their Role in Adherence Mechanisms.- Antigen and Metabolite Detection in Invasive Candidiasis.- Mannan Antigen of Candida albicans and Cellular Immune Responses in vitro and in vivo.- Contributed Papers.- Antigenicity of Candida albicans Cell Wall after Enzymatic Digestion.- Differential Expression of Secretory Proteinase by Candida albicans and C. parapsilosis Invading Human Mucosa.- Production of Anti-Candida Monoclonal Antibodies: Immunopurification of C. albicans Cell Wall Antigens.- Candida Antigen and Arabinitol Levels in the Sera of Patients with Proven or Probable Invasive Candidosis.- Mannan Detection by ELISA-Inhibition in Desseminated Candidiasis: Experimental Study in Rabbits.- Evaluation of the Candidate Super Latex Agglutination Test for the Diagnosis of Vaginal Candidosis.- An Attempt to Obtain Candida Skin Antigens for the Diagnosis of Systemic Candidosis.- Candida Mannan Antigen Detection versus Quantitative Culture in Urine, Sputum, Stool, and Vaginal Swabs.- Systematic Use of a Latex Test to Detect Soluble CandidaAntigens in Serodiagnosis of Systemic Candidiasis.- Evaluation of Immune Complexes and Detection of a Mr48,000 Protein in Candidiasis.- Monoclonal Antibodies: Anti-Candida albicans Mannan and Anti-Aspergillus fumigatus Galactomannan.- Yeast Killer Toxin Monoclonal Antibodies.- A Quantitative Immunofluorescence Test: An Evaluation of Candida Antigen in the Diagnosis of Invasive Candidiasis.- Diagnosis of Systemic Candidosis by Latex Particle Agglutination.- Antigens for the Detection of Candida guilliermondiivar. guilliermondii Infection in Ruminants.- Allergenic Moulds.- Allergens of Aspergillus fumigatus.- Allergens of Alternaria and Cladosporium.- Contributed Papers.- Distinct Cell-Wall and Cytoplasmic Proteinic Antigenic Fractions from Candida albicans Inducing IgE Antibodies in Hypersensitive Patients.- Serial Specific IgG Measurements to Common Moulds.- The Antigenic and Allergenic Properties of Botrytis cinerea.- Cryptococcus.- Correlates of Serotype in Cryptococcus neoformans.- Characterization of Cross-Reactivity between cryptococcal Polysaccharides by Use of Enzyme-Linked Immunosorbent Assays and Monoclonal Antibodies.- Cytoplasmic Antigens of Cryptococcusneoformans.- Contributed Papers.- Characterization, Specificity and Protective Effect of a Murine Monoclonal Antibody Reactive with Cryptococcus neoformans (CN) Capsular Polysaccharide (CNPS).- Kinetic Study of Humoral and Cellular Immune Responses to Cryptococcus neoformans Measured by ELISA and Immunocyte Migration Inhibition.- Serotypes of Clinical and Saprophytic Isolates of Cryptococcusneoformans.- About Soluble Cryptococcal Antigen (SCA): Interest in Screening of Patients with AIDS.- Dermatophytes.- Antigens of Dermatophytes and Their Characterization Using Monoclonal Antibodies.- Antigens Involved in the Pathology of Fonsecaea pedrosoi: Immunochemical Study.- Contributed Papers.- Immunological Aspects of a Keratinase Isolated from Trichophyton mentagrophytes.- Diversity of Antigenic Extracts from the Dermatophyte Trichophyton rubrum.- Monoclonal Antibodies Against Trichophyton rubrum and their Cross-Activity with Related and Non-Related Antigens.- Enzyme-Linked Immunosorbent Assay (ELISA) for Detection of Antibodies Against Pityrosporum orbiculare.- Standardization of Dermatophyte Antigens (Trichophytins) for Skin Test Purposes.- Studies on the Antigens of Pityrosporum (Malassezia) Species.- Aspergillus.- Antigenic Differences Between Exoantigens, Mycelial and Conidial Antigens in Aspergillus.- Characterization of Purified Wall Antigens Obtained from Aspergillus Species.- Antigen Detection in Invasive Aspergillosis.- Contributed Papers.- Aspergillosis in Immunocompromised Patients - Part I: The Problem of Diagnosis.- Aspergillosis in Immunocompromised Patients - Part lis Evaluation of an Enzyme-Linked Immunosorbent Assay (ELISA) for the Detection of AspergillusAntigen.- Immunochemical Studies of Candida and AspergillusAntigenss Taxonomic and Diagnostic Significance.- Interest in Serological Techniques for the Detection and Identification of Phoma exigua, the Agent of Potato Gangrene.- Detection of Aspergillus Glycoprotein in Sera of Patients with Invasive Aspergillosis by an Enzyme-Linked Immunosorbent Assay.- An Enzyme Immunoassay for the Detection of Tenuazonic Acid, a Toxin from the Rice Fungal Pathogen Pyricularia oryzae.- Value and Limits of ELISA in Aspergillosiss Serological Diagnosis in Clinical Forms and Control of a High Risk Population.- A Rapid Double Antibody Biotin-Streptavidin ELISA for Aspergillus fumigatus Glycoprotein Antigen.- Peroxidase Anti-Peroxidase Staining for the Specific Identification of Fungal Antigens in Tissue Sections.- An Assessment of the Assay for Serum Antibodies to Aspergillus fumigatus Catalase in the Diagnostic Laboratory.- Detection of Aspergillus fumigatus Antigenic Components Recognizing IgG, IgM, IgA or IgE Specific Antibodies by ELIFA.- Antigens and Allergens of the Genus Chrysosporium(Emmonsia) Agent of Adiaspiromycosis.- Coccidioides and Histoplasma.- Wall-Associated Antigens of Coccidioides immitis.- Immunochemical Analysis of Histoplasmin Proteins and Polysaccharide.- Immune Responses to Subcellular Antigens of Histoplasma capsulatum.- Contributed Paper.- Recognition of Coccidiodes immitis Antigens with Monoclonal Antibodies.- Actinomycetes.- Antigens of Thermophilic Actinomycetes.